Effect of low-concentration rhamnolipid on transport of Pseudomonas aeruginosa ATCC 9027 in an ideal porous medium with hydrophilic or hydrophobic surfaces

Hua Zhong, Guansheng Liu, Yongbing Jiang, Mark L Brusseau, Zhifeng Liu, Yang Liu, Guangming Zeng

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The success of effective bioaugmentation processes for remediation of soil and groundwater contamination requires effective transport of the injected microorganisms in the subsurface environment. In this study, the effect of low concentrations of monorhamnolipid biosurfactant solutions on transport of Pseudomonas aeruginosa in an ideal porous medium (glass beads) with hydrophilic or hydrophobic surfaces was investigated by conducting miscible-displacement experiments. Transport behavior was examined for both glucose-grown and hexadecane-grown cells, with low and high surface hydrophobicity, respectively. A clean-bed colloid deposition model was used for determination of deposition rate coefficients. Results show that cells with high surface hydrophobicity exhibit greater retention than cells with low surface hydrophobicity. Rhamnolipid affects cell transport primarily by changing cell surface hydrophobicity, with an additional minor effect by increasing solution ionic strength. There is a good linear relation between k and rhamnolipid-regulated cell surface hydrophobicity presented as bacterial-adhesion-to-hydrocarbon (BATH) rate of cells (R2=0.71). The results of this study show the importance of hydrophobic interaction for transport of bacterial cells in silica-based porous media, and the potential of using low-concentration rhamnolipid solutions for facilitating bacterial transport in bioaugmentation efforts.

Original languageEnglish (US)
Pages (from-to)244-248
Number of pages5
JournalColloids and Surfaces B: Biointerfaces
Volume139
DOIs
StatePublished - Mar 1 2016

Keywords

  • Bacterial transport
  • Cell surface hydrophobicity
  • Deposition
  • Hydrophobic interaction
  • Rhamnolipid

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

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