Effect of mild heat stress on the proliferative and differentiative ability of human mesenchymal stromal cells

Mahmood S. Choudhery, Michael Badowski, Angela Muise, David T. Harris

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Background aims: Mesenchymal stromal cells (MSCs) are an attractive candidate for autologous cell therapy, but regenerative potential can be compromised with extensive invitro cell passaging. Development of viable cell therapies must address the effect of invitro passaging to maintain overall functionality of expanded MSCs. Methods: We examined the effect of repeated mild heat shock on the proliferation and differentiation capability of human adipose-derived MSCs. Adipose tissue MSCs were characterized by means of fluorescence activated cell sorting analysis for expression of CD3, CD14, CD19, CD34, CD44, CD45, CD73, CD90 and CD105. Similarly, the expression of SIRT-1, p16INK4a and p21 was determined by means of polymerase chain reaction. Measurements of population doubling, doubling time and superoxide dismutase activity were also determined. Differentiation of expanded MSCs into bone and adipose were analyzed qualitatively and quantitatively. Results: The strategy led to an increase in expression of SIRT-1 concomitant with enhanced viability, proliferation and delayed senescence. The stressed MSCs showed better differentiation into osteoblasts and adipocytes. Conclusions: The results indicate that mild heat shock could be used to maintain MSC proliferative and differentiation potential.

Original languageEnglish (US)
Pages (from-to)359-368
Number of pages10
JournalCytotherapy
Volume17
Issue number4
DOIs
StatePublished - Apr 1 2015

Keywords

  • Heat shock
  • Mesenchymal stromal cells
  • Senescence

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Oncology
  • Genetics(clinical)
  • Cell Biology
  • Cancer Research
  • Transplantation

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