Effect of Mosquito Salivary Gland Treatment on Vesicular Stomatitis New Jersey Virus Replication and Interferon α/β Expression In Vitro

Kirsten Limesand, S. Higgs, L. D. Pearson, B. J. Beaty

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The sensitivity of vesicular stomatitis (VS) viruses to Interferon (IFN)-mediated anti-viral effects has been well documented. Previous studies in our laboratory have shown the ability of mosquito saliva to enhance vesicular stomatitis New Jersey (VSNJ) virus infection in mice. To investigate the effect of mosquito saliva on virus replication and IFN α/β expression, virus titers were analyzed at various time points after infection in cells that were treated with mosquito salivary gland homogenate (SGH). Salivary gland treatment of mouse fibroblast cells (L929) resulted in a significant increase in virus growth kinetics compared with untreated controls. In contrast, Vero cells, which are deficient in the IFN α/β response, did not yield increased viral titers in the time points examined. Treatment of L929 cells with an IFN α/β neutralizing antibody also slightly increased virus yield. Ribonuclease protection assays revealed that induction of IFN α2 expression was reduced in L929 cells treated with SGH. Modulation of IFN α/β by mosquito saliva may be a critical determinant of the transmission and pathogenesis of VSNJ virus.

Original languageEnglish (US)
Pages (from-to)199-205
Number of pages7
JournalJournal of Medical Entomology
Volume40
Issue number2
StatePublished - Mar 2003
Externally publishedYes

Fingerprint

Vesicular stomatitis New Jersey virus
interferons
Virus Replication
virus replication
salivary glands
Salivary Glands
Culicidae
Interferons
saliva
Saliva
viral load
Viruses
cells
Vesiculovirus
viruses
Vesicular Stomatitis
Vero Cells
ribonucleases
mice
Virus Diseases

Keywords

  • Interferon α/β
  • Mosquito
  • Saliva
  • Vesicular stomatitis virus

ASJC Scopus subject areas

  • Insect Science
  • veterinary(all)

Cite this

Effect of Mosquito Salivary Gland Treatment on Vesicular Stomatitis New Jersey Virus Replication and Interferon α/β Expression In Vitro. / Limesand, Kirsten; Higgs, S.; Pearson, L. D.; Beaty, B. J.

In: Journal of Medical Entomology, Vol. 40, No. 2, 03.2003, p. 199-205.

Research output: Contribution to journalArticle

@article{2f8089e4b1d34841ba7ee89d7c1da5b7,
title = "Effect of Mosquito Salivary Gland Treatment on Vesicular Stomatitis New Jersey Virus Replication and Interferon α/β Expression In Vitro",
abstract = "The sensitivity of vesicular stomatitis (VS) viruses to Interferon (IFN)-mediated anti-viral effects has been well documented. Previous studies in our laboratory have shown the ability of mosquito saliva to enhance vesicular stomatitis New Jersey (VSNJ) virus infection in mice. To investigate the effect of mosquito saliva on virus replication and IFN α/β expression, virus titers were analyzed at various time points after infection in cells that were treated with mosquito salivary gland homogenate (SGH). Salivary gland treatment of mouse fibroblast cells (L929) resulted in a significant increase in virus growth kinetics compared with untreated controls. In contrast, Vero cells, which are deficient in the IFN α/β response, did not yield increased viral titers in the time points examined. Treatment of L929 cells with an IFN α/β neutralizing antibody also slightly increased virus yield. Ribonuclease protection assays revealed that induction of IFN α2 expression was reduced in L929 cells treated with SGH. Modulation of IFN α/β by mosquito saliva may be a critical determinant of the transmission and pathogenesis of VSNJ virus.",
keywords = "Interferon α/β, Mosquito, Saliva, Vesicular stomatitis virus",
author = "Kirsten Limesand and S. Higgs and Pearson, {L. D.} and Beaty, {B. J.}",
year = "2003",
month = "3",
language = "English (US)",
volume = "40",
pages = "199--205",
journal = "Journal of Medical Entomology",
issn = "0022-2585",
publisher = "Entomological Society of America",
number = "2",

}

TY - JOUR

T1 - Effect of Mosquito Salivary Gland Treatment on Vesicular Stomatitis New Jersey Virus Replication and Interferon α/β Expression In Vitro

AU - Limesand, Kirsten

AU - Higgs, S.

AU - Pearson, L. D.

AU - Beaty, B. J.

PY - 2003/3

Y1 - 2003/3

N2 - The sensitivity of vesicular stomatitis (VS) viruses to Interferon (IFN)-mediated anti-viral effects has been well documented. Previous studies in our laboratory have shown the ability of mosquito saliva to enhance vesicular stomatitis New Jersey (VSNJ) virus infection in mice. To investigate the effect of mosquito saliva on virus replication and IFN α/β expression, virus titers were analyzed at various time points after infection in cells that were treated with mosquito salivary gland homogenate (SGH). Salivary gland treatment of mouse fibroblast cells (L929) resulted in a significant increase in virus growth kinetics compared with untreated controls. In contrast, Vero cells, which are deficient in the IFN α/β response, did not yield increased viral titers in the time points examined. Treatment of L929 cells with an IFN α/β neutralizing antibody also slightly increased virus yield. Ribonuclease protection assays revealed that induction of IFN α2 expression was reduced in L929 cells treated with SGH. Modulation of IFN α/β by mosquito saliva may be a critical determinant of the transmission and pathogenesis of VSNJ virus.

AB - The sensitivity of vesicular stomatitis (VS) viruses to Interferon (IFN)-mediated anti-viral effects has been well documented. Previous studies in our laboratory have shown the ability of mosquito saliva to enhance vesicular stomatitis New Jersey (VSNJ) virus infection in mice. To investigate the effect of mosquito saliva on virus replication and IFN α/β expression, virus titers were analyzed at various time points after infection in cells that were treated with mosquito salivary gland homogenate (SGH). Salivary gland treatment of mouse fibroblast cells (L929) resulted in a significant increase in virus growth kinetics compared with untreated controls. In contrast, Vero cells, which are deficient in the IFN α/β response, did not yield increased viral titers in the time points examined. Treatment of L929 cells with an IFN α/β neutralizing antibody also slightly increased virus yield. Ribonuclease protection assays revealed that induction of IFN α2 expression was reduced in L929 cells treated with SGH. Modulation of IFN α/β by mosquito saliva may be a critical determinant of the transmission and pathogenesis of VSNJ virus.

KW - Interferon α/β

KW - Mosquito

KW - Saliva

KW - Vesicular stomatitis virus

UR - http://www.scopus.com/inward/record.url?scp=0038361348&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038361348&partnerID=8YFLogxK

M3 - Article

VL - 40

SP - 199

EP - 205

JO - Journal of Medical Entomology

JF - Journal of Medical Entomology

SN - 0022-2585

IS - 2

ER -