Effect of pectin methylesterase gene expression on pea root development

Fushi Wen, Yanmin Zhu, Martha C Hawes

Research output: Contribution to journalArticle

181 Citations (Scopus)

Abstract

Expression of an inducible gene with sequences common to genes encoding pectin methylesterase (PME) was found to be tightly correlated, both spatially and temporally, with border cell separation in pea root caps. Partial inhibition of the gene's expression by antisense mRNA in transgenic pea hairy roots prevented the normal separation of root border cells from the root tip into the external environment. This phenotype was correlated with an increase in extracellular pH, reduced root elongation, and altered cellular morphology. The translation product of the gene exhibited PME activity in vitro. These results are consistent with the long-standing hypothesis that the demethylation of pectin by PME plays a key role in cell wall metabolism.

Original languageEnglish (US)
Pages (from-to)1129-1140
Number of pages12
JournalPlant Cell
Volume11
Issue number6
DOIs
StatePublished - Jun 1999

Fingerprint

pectinesterase
Peas
Gene expression
peas
Gene Expression
gene expression
Genes
root cap
Gene encoding
genes
Meristem
Cell Separation
root tips
Metabolism
pectins
Cell Wall
translation (genetics)
Elongation
root growth
Cells

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Effect of pectin methylesterase gene expression on pea root development. / Wen, Fushi; Zhu, Yanmin; Hawes, Martha C.

In: Plant Cell, Vol. 11, No. 6, 06.1999, p. 1129-1140.

Research output: Contribution to journalArticle

Wen, Fushi ; Zhu, Yanmin ; Hawes, Martha C. / Effect of pectin methylesterase gene expression on pea root development. In: Plant Cell. 1999 ; Vol. 11, No. 6. pp. 1129-1140.
@article{3523c6af97784058bcf1270d5d09d2a6,
title = "Effect of pectin methylesterase gene expression on pea root development",
abstract = "Expression of an inducible gene with sequences common to genes encoding pectin methylesterase (PME) was found to be tightly correlated, both spatially and temporally, with border cell separation in pea root caps. Partial inhibition of the gene's expression by antisense mRNA in transgenic pea hairy roots prevented the normal separation of root border cells from the root tip into the external environment. This phenotype was correlated with an increase in extracellular pH, reduced root elongation, and altered cellular morphology. The translation product of the gene exhibited PME activity in vitro. These results are consistent with the long-standing hypothesis that the demethylation of pectin by PME plays a key role in cell wall metabolism.",
author = "Fushi Wen and Yanmin Zhu and Hawes, {Martha C}",
year = "1999",
month = "6",
doi = "10.1105/tpc.11.6.1129",
language = "English (US)",
volume = "11",
pages = "1129--1140",
journal = "Plant Cell",
issn = "1040-4651",
publisher = "American Society of Plant Biologists",
number = "6",

}

TY - JOUR

T1 - Effect of pectin methylesterase gene expression on pea root development

AU - Wen, Fushi

AU - Zhu, Yanmin

AU - Hawes, Martha C

PY - 1999/6

Y1 - 1999/6

N2 - Expression of an inducible gene with sequences common to genes encoding pectin methylesterase (PME) was found to be tightly correlated, both spatially and temporally, with border cell separation in pea root caps. Partial inhibition of the gene's expression by antisense mRNA in transgenic pea hairy roots prevented the normal separation of root border cells from the root tip into the external environment. This phenotype was correlated with an increase in extracellular pH, reduced root elongation, and altered cellular morphology. The translation product of the gene exhibited PME activity in vitro. These results are consistent with the long-standing hypothesis that the demethylation of pectin by PME plays a key role in cell wall metabolism.

AB - Expression of an inducible gene with sequences common to genes encoding pectin methylesterase (PME) was found to be tightly correlated, both spatially and temporally, with border cell separation in pea root caps. Partial inhibition of the gene's expression by antisense mRNA in transgenic pea hairy roots prevented the normal separation of root border cells from the root tip into the external environment. This phenotype was correlated with an increase in extracellular pH, reduced root elongation, and altered cellular morphology. The translation product of the gene exhibited PME activity in vitro. These results are consistent with the long-standing hypothesis that the demethylation of pectin by PME plays a key role in cell wall metabolism.

UR - http://www.scopus.com/inward/record.url?scp=0033149816&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033149816&partnerID=8YFLogxK

U2 - 10.1105/tpc.11.6.1129

DO - 10.1105/tpc.11.6.1129

M3 - Article

C2 - 10368183

AN - SCOPUS:0033149816

VL - 11

SP - 1129

EP - 1140

JO - Plant Cell

JF - Plant Cell

SN - 1040-4651

IS - 6

ER -