Abstract
Lowering extravesicular pH stimulated Na+-dependent citrate transport in renal brush border membrane vesicles: e.g., at pHout = 5.5, the initial rate of citrate uptake was increased 10-fold compared to parallel control experiments at pH 7.5. The same experimental conditions had little effect on succinate uptake. The influence of pH on citrate transport is a product of the extravesicular H+ concentration; pH gradients did not potentiate the effects nor were proton gradients capable of driving transport in the absence of Na+. The effect of pH is adequately explained if only the mono- and divalent species of citrate (Cit1-, Cit2-) are considered acceptable substrates for transport. The stimulatory influence of pH on transport correlated quite well with pH-related increases in the concentrations of Cit1- and Cit2-, and over the same pH range [Cit3-] was inversely related to citrate uptake. A model of the Na+-dependent dicarboxylate transport system is discussed in which three sodium ions are translocated per molecule of dicarboxylic acid.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 287-290 |
| Number of pages | 4 |
| Journal | BBA - Biomembranes |
| Volume | 684 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 22 1982 |
| Externally published | Yes |
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Keywords
- (Rabbit kidney)
- Brush border membrane
- Citrate transport
- pH effect
- Tricarboxylic acid cycle
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
- Medicine(all)
Cite this
Effect of pH on the transport of Krebs cycle intermediates in renal brush border membranes. / Wright, Stephen; Kippen, Ian; Wright, Ernest M.
In: BBA - Biomembranes, Vol. 684, No. 2, 22.01.1982, p. 287-290.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of pH on the transport of Krebs cycle intermediates in renal brush border membranes
AU - Wright, Stephen
AU - Kippen, Ian
AU - Wright, Ernest M.
PY - 1982/1/22
Y1 - 1982/1/22
N2 - Lowering extravesicular pH stimulated Na+-dependent citrate transport in renal brush border membrane vesicles: e.g., at pHout = 5.5, the initial rate of citrate uptake was increased 10-fold compared to parallel control experiments at pH 7.5. The same experimental conditions had little effect on succinate uptake. The influence of pH on citrate transport is a product of the extravesicular H+ concentration; pH gradients did not potentiate the effects nor were proton gradients capable of driving transport in the absence of Na+. The effect of pH is adequately explained if only the mono- and divalent species of citrate (Cit1-, Cit2-) are considered acceptable substrates for transport. The stimulatory influence of pH on transport correlated quite well with pH-related increases in the concentrations of Cit1- and Cit2-, and over the same pH range [Cit3-] was inversely related to citrate uptake. A model of the Na+-dependent dicarboxylate transport system is discussed in which three sodium ions are translocated per molecule of dicarboxylic acid.
AB - Lowering extravesicular pH stimulated Na+-dependent citrate transport in renal brush border membrane vesicles: e.g., at pHout = 5.5, the initial rate of citrate uptake was increased 10-fold compared to parallel control experiments at pH 7.5. The same experimental conditions had little effect on succinate uptake. The influence of pH on citrate transport is a product of the extravesicular H+ concentration; pH gradients did not potentiate the effects nor were proton gradients capable of driving transport in the absence of Na+. The effect of pH is adequately explained if only the mono- and divalent species of citrate (Cit1-, Cit2-) are considered acceptable substrates for transport. The stimulatory influence of pH on transport correlated quite well with pH-related increases in the concentrations of Cit1- and Cit2-, and over the same pH range [Cit3-] was inversely related to citrate uptake. A model of the Na+-dependent dicarboxylate transport system is discussed in which three sodium ions are translocated per molecule of dicarboxylic acid.
KW - (Rabbit kidney)
KW - Brush border membrane
KW - Citrate transport
KW - pH effect
KW - Tricarboxylic acid cycle
UR - http://www.scopus.com/inward/record.url?scp=0020054827&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020054827&partnerID=8YFLogxK
U2 - 10.1016/0005-2736(82)90019-0
DO - 10.1016/0005-2736(82)90019-0
M3 - Article
VL - 684
SP - 287
EP - 290
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
SN - 0005-2736
IS - 2
ER -