Effect of reproductive hormones on ovarian epithelial tumors: II. Effect on angiogenic activity

Chen Chen, Eric Petitclerc, Hong Zhou, Peter C. Brooks, Tong Sun, Mimi C. Yu, Wenxin - Zheng, Louis Dubeau

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Menstrual cycle activity predisposes to ovarian epithelial tumors based on numerous epidemiological studies. We showed that the hormones involved in menstrual cycle regulation modulate cell cycle activity in these tumors in an accompanying paper. We investigated whether such hormones could also influence angiogenesis, an important determinant of tumor progression, in the same tumors. Treatment with progesterone (P4) stimulated VEGF protein secretion in 4 of 5 ovarian carcinoma cell lines examined. Northern blot analyses performed in MCV50 cells showed that this effect was accompanied by increased VEGF mRNA levels. P4 also stimulated VEGF promoter activity in these cells. Estradiol (E2) showed a similar, but substantially smaller effect on VEGF secretion which was additive to that of P4. Conditioned medium from P4-treated cells strongly stimulated angiogenesis on chicken chorio-allantoic membranes. Incubating the conditioned medium with a neutralizing anti-VEGF antibody, but not with non-specific immunoglobulins abolished this effect. Angiogenic activity was not altered by treatment of the membranes with P4 directly. We conclude that P4 can stimulate angiogenic activity via induction of VEGF secretion in some ovarian epithelial tumors. Therapeutic use of progestins may be most effective when administered in combination with an anti-angiogenic agent, at least against a subset of ovarian carcinomas.

Original languageEnglish (US)
Pages (from-to)307-312
Number of pages6
JournalCancer Biology and Therapy
Volume1
Issue number3
StatePublished - May 2002
Externally publishedYes

Fingerprint

Vascular Endothelial Growth Factor A
Hormones
Neoplasms
Menstrual Cycle
Conditioned Culture Medium
Allantois
Carcinoma
Therapeutic Uses
Progestins
Neutralizing Antibodies
Northern Blotting
Progesterone
Immunoglobulins
Epidemiologic Studies
Anti-Idiotypic Antibodies
Chickens
Estradiol
Cell Cycle
Cell Line
Messenger RNA

Keywords

  • Angiogenesis
  • Hormones
  • Menstrual cycle
  • Ovarian neoplasia

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Molecular Medicine
  • Pharmacology

Cite this

Chen, C., Petitclerc, E., Zhou, H., Brooks, P. C., Sun, T., Yu, M. C., ... Dubeau, L. (2002). Effect of reproductive hormones on ovarian epithelial tumors: II. Effect on angiogenic activity. Cancer Biology and Therapy, 1(3), 307-312.

Effect of reproductive hormones on ovarian epithelial tumors : II. Effect on angiogenic activity. / Chen, Chen; Petitclerc, Eric; Zhou, Hong; Brooks, Peter C.; Sun, Tong; Yu, Mimi C.; Zheng, Wenxin -; Dubeau, Louis.

In: Cancer Biology and Therapy, Vol. 1, No. 3, 05.2002, p. 307-312.

Research output: Contribution to journalArticle

Chen, C, Petitclerc, E, Zhou, H, Brooks, PC, Sun, T, Yu, MC, Zheng, W & Dubeau, L 2002, 'Effect of reproductive hormones on ovarian epithelial tumors: II. Effect on angiogenic activity', Cancer Biology and Therapy, vol. 1, no. 3, pp. 307-312.
Chen C, Petitclerc E, Zhou H, Brooks PC, Sun T, Yu MC et al. Effect of reproductive hormones on ovarian epithelial tumors: II. Effect on angiogenic activity. Cancer Biology and Therapy. 2002 May;1(3):307-312.
Chen, Chen ; Petitclerc, Eric ; Zhou, Hong ; Brooks, Peter C. ; Sun, Tong ; Yu, Mimi C. ; Zheng, Wenxin - ; Dubeau, Louis. / Effect of reproductive hormones on ovarian epithelial tumors : II. Effect on angiogenic activity. In: Cancer Biology and Therapy. 2002 ; Vol. 1, No. 3. pp. 307-312.
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