Effect of resistance to Bacillus thuringiensis cotton on pink bollworm (Lepidoptera: Gelechiidae) response to sex pheromone

Yves Carrière, Megan E. Nyboer, Christa Ellers-Kirk, James Sollome, Nick Colletto, Larry Antilla, Timothy J. Dennehy, Robert T. Staten, Bruce E. Tabashnik

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Fitness costs associated with resistance to transgenic crops producing toxins from Bacillus thuringiensis (Bt) could reduce male response to pheromone traps. Such costs would cause underestimation of resistance frequency if monitoring was based on analysis of males caught in pheromone traps. To develop a DNA-based resistance monitoring program for pink bollworm, Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae), we compared the response to pheromone traps of males with and without cadherin alleles associated with resistance to Bt cotton (Gossypium hirsutum L.). When irradiated males from two hybrid laboratory strains with an inter-mediate frequency of resistance alleles were released in large field cages, the probability of capture in pheromone traps was not lower for males with resistance alleles than for males without resistance alleles. These results suggest that analysis of trapped males would not underestimate the frequency of resistance. As the time males spent in traps in the field increased from 3 to 15 d, the success of DNA amplification declined from 100 to 30%. Thus, the efficiency of a DNA-based resistance monitoring program would be improved by analyzing males remaining in traps for 3 d or less.

Original languageEnglish (US)
Pages (from-to)946-953
Number of pages8
JournalJournal of economic entomology
Volume99
Issue number3
StatePublished - Jun 1 2006
Externally publishedYes

Keywords

  • Bacillus thuringiensis crops
  • Cadherin
  • Molecular monitoring
  • Pectinophora gossypiella
  • Resistance detection

ASJC Scopus subject areas

  • Ecology
  • Insect Science

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