Effect of sub-micron pillar array on DNA kinetics in a free-solution capillary electrophoresis microsystem

Yick Chuen Chan, Yi Kuen Lee, Man Wong, Yitshak Zohar

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This paper presents an experimental and numerical study of the effect of a sub-micron pillar array, embedded in the separation channel, on DNA kinetics in a free-solution capillary electrophoresis microsystem. DNA plugs have been observed to migrate with different velocity depending on the cross-sectional shape of the pillars. This velocity variation can be partly explained by the electric field re-distribution due to the pillar array. Numerical simulations have been carried out to verify the effect of the sub-micron pillar geometry on the electric field distribution, and the computations of DNA plug velocity are qualitatively consistent with the measurements.

Original languageEnglish (US)
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages337-339
Number of pages3
Volume1
ISBN (Print)0974361119, 9780974361116
StatePublished - 2005
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: Oct 9 2005Oct 13 2005

Other

Other9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
CountryUnited States
CityBoston, MA
Period10/9/0510/13/05

Fingerprint

Capillary electrophoresis
Microsystems
DNA
Kinetics
Electric fields
Geometry
Computer simulation

Keywords

  • DNA kinetics
  • Electrophoresis
  • Free-solution
  • Sub-micron pillars

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Chan, Y. C., Lee, Y. K., Wong, M., & Zohar, Y. (2005). Effect of sub-micron pillar array on DNA kinetics in a free-solution capillary electrophoresis microsystem. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences (Vol. 1, pp. 337-339). Transducer Research Foundation.

Effect of sub-micron pillar array on DNA kinetics in a free-solution capillary electrophoresis microsystem. / Chan, Yick Chuen; Lee, Yi Kuen; Wong, Man; Zohar, Yitshak.

Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Vol. 1 Transducer Research Foundation, 2005. p. 337-339.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Chan, YC, Lee, YK, Wong, M & Zohar, Y 2005, Effect of sub-micron pillar array on DNA kinetics in a free-solution capillary electrophoresis microsystem. in Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. vol. 1, Transducer Research Foundation, pp. 337-339, 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005, Boston, MA, United States, 10/9/05.
Chan YC, Lee YK, Wong M, Zohar Y. Effect of sub-micron pillar array on DNA kinetics in a free-solution capillary electrophoresis microsystem. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Vol. 1. Transducer Research Foundation. 2005. p. 337-339
Chan, Yick Chuen ; Lee, Yi Kuen ; Wong, Man ; Zohar, Yitshak. / Effect of sub-micron pillar array on DNA kinetics in a free-solution capillary electrophoresis microsystem. Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Vol. 1 Transducer Research Foundation, 2005. pp. 337-339
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AB - This paper presents an experimental and numerical study of the effect of a sub-micron pillar array, embedded in the separation channel, on DNA kinetics in a free-solution capillary electrophoresis microsystem. DNA plugs have been observed to migrate with different velocity depending on the cross-sectional shape of the pillars. This velocity variation can be partly explained by the electric field re-distribution due to the pillar array. Numerical simulations have been carried out to verify the effect of the sub-micron pillar geometry on the electric field distribution, and the computations of DNA plug velocity are qualitatively consistent with the measurements.

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