Effect of substrate mechanics on cardiomyocyte maturation and growth

Marwa Tallawi, Ranjana Rai, Aldo R. Boccaccini, Katerina E Aifantis

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Cardiac tissue engineering constructs are a promising therapeutic treatment for myocardial infarction, which is one of the leading causes of death. In order to further advance the development and regeneration of engineered cardiac tissues using biomaterial platforms, it is important to have a complete overview of the effects that substrates have on cardiomyocyte (CM) morphology and function. This article summarizes recent studies that investigate the effect of mechanical cues on the CM differentiation, maturation, and growth. In these studies, CMs derived from embryos, neonates, and mesenchymal stem cells were seeded on different substrates of various elastic modulus. Measuring the contractile function by force production, work output, and calcium handling, it was seen that cell behavior on substrates was optimized when the substrate stiffness mimicked that of the native tissue. The contractile function reflected changes in the sarcomeric protein confirmation and organization that promoted the contractile ability. The analysis of the literature also revealed that, in addition to matrix stiffness, mechanical stimulation, such as stretching the substrate during cell seeding, also played an important role during cell maturation and tissue development.

Original languageEnglish (US)
Pages (from-to)157-165
Number of pages9
JournalTissue Engineering - Part B: Reviews
Volume21
Issue number1
DOIs
StatePublished - Feb 1 2015

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Mechanics
Cardiac Myocytes
Substrates
Growth
Tissue
Aptitude
Elastic Modulus
Biocompatible Materials
Tissue Engineering
Mesenchymal Stromal Cells
Cues
Regeneration
Cause of Death
Embryonic Structures
Myocardial Infarction
Organizations
Calcium
Stiffness matrix
Stem cells
Tissue engineering

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Bioengineering
  • Biochemistry

Cite this

Effect of substrate mechanics on cardiomyocyte maturation and growth. / Tallawi, Marwa; Rai, Ranjana; Boccaccini, Aldo R.; Aifantis, Katerina E.

In: Tissue Engineering - Part B: Reviews, Vol. 21, No. 1, 01.02.2015, p. 157-165.

Research output: Contribution to journalArticle

Tallawi, Marwa ; Rai, Ranjana ; Boccaccini, Aldo R. ; Aifantis, Katerina E. / Effect of substrate mechanics on cardiomyocyte maturation and growth. In: Tissue Engineering - Part B: Reviews. 2015 ; Vol. 21, No. 1. pp. 157-165.
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