Effect of the recreational agent Isobutyl nitrite on human peripheral blood leukocytes and on in vitro interferon production

Evan M. Hersh, James M. Reuben, Hal Bogerd, Michael Rosenblum, Marc Bielski, A. Adan, Gerald Sonnenfeld, Peter W.A. Mansell, Guy R. Newell

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Abstract

The effects of the isobutyl nitrite sold as incense and the chemically pure compound on various in vitro parameters of leukocyte function were studied. This was done because of the potential relationship of isobutyl nitrite use to the opportunistic infection and Kaposi's sarcoma seen in homosexual men. Various concentrations of isobutyl nitrite dissolved in ethyl alcohol were added to various leukocyte cultures. The final added concentrations were 0.001 to 1.0%. Because of the poor solubility of the agent, the fluid concentrations were quite low after addition. Thus, the measured concentrations in the medium after preparation of 1% (v/v) solution were 0.45 mM at 1 hr, 0.04 mM at 24 hr, and 0.04 mM at 48 hr of incubation at 37° in 5% CO2in air. At the 1% added concentration, the agent lysed leukocytes and reduced viability from 95% to 21% in 24 hr. At an added concentration of 0.5% or below, cell count and viability were unaffected, but the agent inhibited in vitro lymphocyte blastogenic responses to phytohemagglutinin, pokeweed mitogen, and concanavalin A. It also inhibited natural killer cell activity to the K562 cell line, lymphocyte-medi-ated antibody-dependent cellular cytotoxicity to the GEM cell line, monocyte-mediated antibody-dependent cellular cytotoxicity to human red blood cells, and in vitro adherence and transformation of monocytes to macrophages. Inhibitory effects were greater than 90% at the 0.5% concentration and were still detectable at 0.01%. Chemically pure isobutyl nitrite and the form sold as incense had identical effects. The agent volatilized from the tissue culture medium at 37° so that its effect on cell viability was reduced about one-third after 24 hr and was gone by 48 hr. The agent inhibited leucine, uridine, and thymidine incorporation approximately equally. Within 2 hr of exposure to isobutyl nitrite, uridine and leucine incorporation were markedly inhibited. After 24 hr of exposure to the agent, the effects on various lymphocyte function parameters were not reversible. The thymidine incorporation of myeloid and solid tumor cell lines was also inhibited by the same concentrations of isobutyl nitrite which inhibited leukocyte functions. Induction of α, β-interferon by polyriboinosinic-polyribocytidylic acid in mouse embryo fibroblasts was inhibited by pretreatment of the cells with isobutyl nitrite. These data suggest that isobutyl nitrite has nonspecific cytotoxic activity for various cells in vitro and could have immunosuppressive effects on tissues exposed in vivo during its recreational use. We speculate that these immunosuppressive effects, combined with the ability of nitrites to convert amines to nitrosamines, may be related to the development of opportunistic infections and Kaposi's sarcoma in homosexuals who use this agent.

Original languageEnglish (US)
Pages (from-to)1365-1371
Number of pages7
JournalCancer Research
Volume43
Issue number3
StatePublished - Mar 1 1983

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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