Effect of VIP on the glycogen metabolism of human colon adenocarcinoma cells studied by 13C nuclear magnetic resonance spectroscopy

Jean-Philippe Galons, J. Fantini, J. Vion-Dury, P. J. Cozzone, P. Canioi

Research output: Contribution to journalArticle

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Abstract

Metabolic pathways of glucose utilization have been investigated in a human colon adenocarcinoma cell line (HT29) using carbon-13 Nuclear Magnetic Resonance spectroscopy. HT29 cells were adapted to grow on a polystyrene beaded microcarrier and were perfused when attached to the beads in a specifically designed NMR cell. Abnormalites in carbohydrate metabolism already observed in several cancer cell were studied in HT29 cells fed with (I-13C)-enriched glucose. The cells were first perfused with a glucose-free medium for 2 hr in order to deplete the intracellular store of glycogen, and they were subsequently perfused with a medium containing enriched glucose at an initial concentration of 5.5 mM. Sequential 13C-NMR spectra, recorded at 100.5 MHz (5 min accumulation), show that HT29 cells were able to utilize glucose through the glycolytic pathway while storing glucose as glycogen (glucose was utilized at a rate of 3.9 μmol/mg protein/hr). The glycolytic activity determined by the amount of lactic acid produced was 4.6 μM/mg protein/hr, corresponding to the formation of 1.2 lactic acid per glucose molecule. Glycogen accumulation corresponded to 16 μg/mg of protein. Treatment of HT29 with 10 nM vasoactive intestinal peptide (VIP) induced a transient decrease in the level of labelled glycogen to 50% of the initial value. Control level was recovered 12 min after VIP loading.

Original languageEnglish (US)
Pages (from-to)168-173
Number of pages6
JournalInternational Journal of Cancer
Volume45
Issue number1
DOIs
StatePublished - 1990
Externally publishedYes

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Vasoactive Intestinal Peptide
Glycogen
Colon
Adenocarcinoma
Magnetic Resonance Spectroscopy
Glucose
HT29 Cells
Lactic Acid
Proteins
Polystyrenes
Carbohydrate Metabolism
Metabolic Networks and Pathways
Cell Line

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Effect of VIP on the glycogen metabolism of human colon adenocarcinoma cells studied by 13C nuclear magnetic resonance spectroscopy. / Galons, Jean-Philippe; Fantini, J.; Vion-Dury, J.; Cozzone, P. J.; Canioi, P.

In: International Journal of Cancer, Vol. 45, No. 1, 1990, p. 168-173.

Research output: Contribution to journalArticle

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