Effects of [1-Nα-trinitrophenylhistidine, 12-homoarginine]glucagon on cyclic AMP levels and free fatty acid release in isolated rat adipocytes

J. Adolfo García-Sáinz, Lourdes Sánchez-Sevilla, John T. Pelton, Dev Trivedi, Victor J. Hruby

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[1-Nα-Trinitrophenylhistidine, 12-homoarginine]glucagon (THG) stimulated, in a concentration-dependent fashion, lipolysis (2-fold) and cyclic AMP accumulation (50% over basal) in isolated rat adipocytes, but was much less effective than glucagon, which stimulated lipolysis 4-fold and cyclic AMP accumulation 10-15-fold. THG displaced to the right the concentration-response curves for glucagon and diminished in a concentration-dependent fashion the effects of a fixed concentration of glucagon. The data indicate that THG is a mixed agonist-antagonist (partial agonist) in isolated rat fat cells.

Original languageEnglish (US)
Pages (from-to)310-315
Number of pages6
JournalBBA - Molecular Cell Research
Issue number2
StatePublished - Apr 29 1986



  • (Rat adipocyte)
  • Chemical modification
  • Glucagon agonist
  • Glucagon analog
  • Glucagon antagonist
  • cyclic AMP accumulation

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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