Effects of 1,3-di-o-tolylguanidine (DTG), a sigma ligand, on local cerebral glucose utilization in rat brain

A. G. Hohmann, R. R. Matsumoto, M. K. Hemstreet, S. L. Patrick, J. E. Margulies, R. P. Hammer, J. M. Walker

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The 2-deoxy-d-[1-14C]glucose ([14C]DG) method was used to examine the effects of the relatively selective sigma ligand 1,3-di-o-tolylguanidine (DTG) on cerebral metabolism in freely moving rats. Each animal received an i.p. injection of DTG (0.2, 1, or 5 mg/kg) or normal saline 20 min prior to the infusion of [14C]DG. DTG induced dose-dependent changes in local cerebral glucose utilization (LCGU) in several motor and limbic structures. Most structures showed increases in LCGU, with a maximum effect at 1 mg/kg. The most profound increases in LCGU were observed in brain regions that are rich in sigma receptors. These included cerebellar and related nuclei (interpositus, lateral and medial cerebellar n., vestibular n., olivary n.), ambiguus n., superior colliculus (superior layers), hippocampus (CA2, CA3, DG), n, basalis of Meynert interpeduncular n., and the substantia nigra pars compacta and pars reticula. No significant decreases in glucose utilization were observed at any dose. Although the areas affected by DTG are similar to those previously reported for other sigma ligands13, future studies employing a range of doses for additional selective sigma ligands must be carried out in order to confirm whether these changes in LCGU were sigma-mediated.

Original languageEnglish (US)
Pages (from-to)265-273
Number of pages9
JournalBrain Research
Volume593
Issue number2
DOIs
StatePublished - Oct 16 1992
Externally publishedYes

Keywords

  • 1,3-Di-o-tolylguanidine
  • 2-Deoxy-Deoxy-d-glucose
  • Local cerebral glucose utilization
  • Sigma ligand

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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    Hohmann, A. G., Matsumoto, R. R., Hemstreet, M. K., Patrick, S. L., Margulies, J. E., Hammer, R. P., & Walker, J. M. (1992). Effects of 1,3-di-o-tolylguanidine (DTG), a sigma ligand, on local cerebral glucose utilization in rat brain. Brain Research, 593(2), 265-273. https://doi.org/10.1016/0006-8993(92)91317-8