Effects of a single intrastrand d(GpG) platinum adduct on the strand separating activity of the Escherichia coli proteins RecB and RecA

Giuseppe Villani, Christophe Cazaux, Marie Jeanne Pillaire, Paul E Boehmer

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

RecB and RecA proteins play key roles in the process ofDNA recombination in Escherichia coli and both possess DNA unwinding activities which can displace short regions of duplex DNA in an ATP-dependent manner in vitro. We have examined the effect of the most abundant DNA adduct caused by the chemotherapeutic agent cM-diamminedichloroplatinum(II) on those activities. For this purpose, we have constructed a partially duplex synthetic oligonucleotide containing the intrastrand d(GpG) crosslink positioned at a specific site. We report here that both the DNA strand separating and DNA-dependent ATPase activities of the RecB protein are inhibited by the d(GpG) cis-ddp adduct. In contrast, neither the unwinding nor the ATPase activities of RecA protein appear to be perturbed by this lesion.

Original languageEnglish (US)
Pages (from-to)89-95
Number of pages7
JournalFEBS Letters
Volume333
Issue number1-2
DOIs
StatePublished - Oct 25 1993
Externally publishedYes

Fingerprint

Escherichia coli Proteins
Platinum
Rec A Recombinases
Adenosine Triphosphatases
DNA
DNA Adducts
Oligonucleotides
Escherichia coli
Genetic Recombination
Adenosine Triphosphate
deoxyguanylyl-(3'-5')-guanosine
Proteins

Keywords

  • cis-Diamminedichloroplatinum(II) d(GpG) adduct
  • DNA helicase activity
  • Escherichia coli RecA and RecB proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Effects of a single intrastrand d(GpG) platinum adduct on the strand separating activity of the Escherichia coli proteins RecB and RecA. / Villani, Giuseppe; Cazaux, Christophe; Pillaire, Marie Jeanne; Boehmer, Paul E.

In: FEBS Letters, Vol. 333, No. 1-2, 25.10.1993, p. 89-95.

Research output: Contribution to journalArticle

Villani, Giuseppe ; Cazaux, Christophe ; Pillaire, Marie Jeanne ; Boehmer, Paul E. / Effects of a single intrastrand d(GpG) platinum adduct on the strand separating activity of the Escherichia coli proteins RecB and RecA. In: FEBS Letters. 1993 ; Vol. 333, No. 1-2. pp. 89-95.
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