Effects of anthocyanidin derivative (HK-008) on relaxation in rat perfused mesenterial bed

Katsuo Kamata, Ayako Makino, Noriyasu Kanie, Shu Ichi Oda, Takayuki Matsumoto, Tsuneo Kobayashi, Toyohiko Kikuchi, Masato Nishimura, Toshio Honda

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Anthocyanins, which are responsible for a variety of bright colors (including red, blue, and purple) in fruits, vegetables, and flowers, are consumed as dietary polyphenols. Anthocyanin-containing fruits are thought to decrease coronary heart disease and are used in anti-diabetic preparations. Diabetes is associated with a variety of cardiovascular complications that may be mediated by endothelial dysfunction, and so this study was designed mainly to characterize the influence of a synthesized anthocyanidin derivative (HK-008) over acetylcholine (ACh)-induced relaxation in mesenteric arterial beds isolated from rats. In a glucose-tolerance test in intact rats, HK-008 (30 mg/kg) reduced the glucose level as effectively as the same dose of glibenclamide. The aortic relaxation induced by pinacidil (an ATP-sensitive potassium channel opener) was greatly inhibited by glibenclamide (10 μM), and also significantly inhibited by HK-008 (10 μM). Interestingly, the ACh-induced relaxation in the perfused, preconstricted mesenteric arterial bed was significantly enhanced by HK-008 (10 μM), and this enhancement was significantly attenuated by indomethacin (10 μM). The ACh-induced mesenteric relaxation was impaired by an increase in oxidative stress, viz. superoxide-generating treatment [xanthine oxidase (XO; 0.1 U/ml) plus hypoxanthine (HX; 10 μM)]. However, this impairment was strongly suppressed by HK-008 (10 μM). These results suggest that HK-008 increases endothelium-induced relaxation by suppressing oxidative stress or modulating prostanoids signaling. This compound may therefore be useful against certain cardiovascular disorders.

Original languageEnglish (US)
Pages (from-to)75-88
Number of pages14
JournalJournal of Smooth Muscle Research
Volume42
Issue number2-3
DOIs
StatePublished - 2006
Externally publishedYes

Fingerprint

Anthocyanins
Acetylcholine
Glyburide
Fruit
Oxidative Stress
Pinacidil
KATP Channels
Hypoxanthine
Xanthine Oxidase
Polyphenols
Glucose Tolerance Test
Superoxides
Indomethacin
Vegetables
Prostaglandins
Endothelium
Coronary Disease
Color
Glucose

Keywords

  • Anthocyanidin
  • Endothelium-dependent relaxation
  • Glibenclamide
  • Mesenteric artery
  • Rat

ASJC Scopus subject areas

  • Physiology

Cite this

Effects of anthocyanidin derivative (HK-008) on relaxation in rat perfused mesenterial bed. / Kamata, Katsuo; Makino, Ayako; Kanie, Noriyasu; Oda, Shu Ichi; Matsumoto, Takayuki; Kobayashi, Tsuneo; Kikuchi, Toyohiko; Nishimura, Masato; Honda, Toshio.

In: Journal of Smooth Muscle Research, Vol. 42, No. 2-3, 2006, p. 75-88.

Research output: Contribution to journalArticle

Kamata, K, Makino, A, Kanie, N, Oda, SI, Matsumoto, T, Kobayashi, T, Kikuchi, T, Nishimura, M & Honda, T 2006, 'Effects of anthocyanidin derivative (HK-008) on relaxation in rat perfused mesenterial bed', Journal of Smooth Muscle Research, vol. 42, no. 2-3, pp. 75-88. https://doi.org/10.1540/jsmr.42.75
Kamata, Katsuo ; Makino, Ayako ; Kanie, Noriyasu ; Oda, Shu Ichi ; Matsumoto, Takayuki ; Kobayashi, Tsuneo ; Kikuchi, Toyohiko ; Nishimura, Masato ; Honda, Toshio. / Effects of anthocyanidin derivative (HK-008) on relaxation in rat perfused mesenterial bed. In: Journal of Smooth Muscle Research. 2006 ; Vol. 42, No. 2-3. pp. 75-88.
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