Effects of anthocyanidin derivative (HK-008) on relaxation in rat perfused mesenterial bed

Katsuo Kamata, Ayako Makino, Noriyasu Kanie, Shu Ichi Oda, Takayuki Matsumoto, Tsuneo Kobayashi, Toyohiko Kikuchi, Masato Nishimura, Toshio Honda

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Anthocyanins, which are responsible for a variety of bright colors (including red, blue, and purple) in fruits, vegetables, and flowers, are consumed as dietary polyphenols. Anthocyanin-containing fruits are thought to decrease coronary heart disease and are used in anti-diabetic preparations. Diabetes is associated with a variety of cardiovascular complications that may be mediated by endothelial dysfunction, and so this study was designed mainly to characterize the influence of a synthesized anthocyanidin derivative (HK-008) over acetylcholine (ACh)-induced relaxation in mesenteric arterial beds isolated from rats. In a glucose-tolerance test in intact rats, HK-008 (30 mg/kg) reduced the glucose level as effectively as the same dose of glibenclamide. The aortic relaxation induced by pinacidil (an ATP-sensitive potassium channel opener) was greatly inhibited by glibenclamide (10 μM), and also significantly inhibited by HK-008 (10 μM). Interestingly, the ACh-induced relaxation in the perfused, preconstricted mesenteric arterial bed was significantly enhanced by HK-008 (10 μM), and this enhancement was significantly attenuated by indomethacin (10 μM). The ACh-induced mesenteric relaxation was impaired by an increase in oxidative stress, viz. superoxide-generating treatment [xanthine oxidase (XO; 0.1 U/ml) plus hypoxanthine (HX; 10 μM)]. However, this impairment was strongly suppressed by HK-008 (10 μM). These results suggest that HK-008 increases endothelium-induced relaxation by suppressing oxidative stress or modulating prostanoids signaling. This compound may therefore be useful against certain cardiovascular disorders.

Original languageEnglish (US)
Pages (from-to)75-88
Number of pages14
JournalJournal of Smooth Muscle Research
Volume42
Issue number2-3
DOIs
StatePublished - Oct 18 2006
Externally publishedYes

Keywords

  • Anthocyanidin
  • Endothelium-dependent relaxation
  • Glibenclamide
  • Mesenteric artery
  • Rat

ASJC Scopus subject areas

  • Physiology

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  • Cite this

    Kamata, K., Makino, A., Kanie, N., Oda, S. I., Matsumoto, T., Kobayashi, T., Kikuchi, T., Nishimura, M., & Honda, T. (2006). Effects of anthocyanidin derivative (HK-008) on relaxation in rat perfused mesenterial bed. Journal of Smooth Muscle Research, 42(2-3), 75-88. https://doi.org/10.1540/jsmr.42.75