Effects of chemical sympathectomy with 6-hydroxydopamine on α- and β-adrenoceptors and muscarinic cholinoceptors in rat kidney

Shizuo Yamada, Henry I. Yamamura, William R. Roeske

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

The autonomic receptors in the rat kidney were characterized using the radioligands [3H]prazosin, [3H]clonidine, [3H]dihydroalprenolol (DHA) and [3H]quinuclidinyl benzilate (QNB). The specific binding of [3H]prazosin, [3H]clonidine, [3H]DHA and [3H]QNB to rat kidney membranes was saturable and of high affinity, and showed a pharmacological specificity as well as stereospecificity which characterized renal α1-, α2- and β-adrenoceptors and muscarinic cholinoceptors, respectively. There was a relatively greater density of α-adrenoceptors than β-adrenoceptors or muscarinic cholinoceptors in the rat kidney. Chemical sympathectomy of rats with 6-hydroxydopamine. HBr (6-OHDA, 50 × 2 mg/kg i.v., 24 h interval) caused a significant increase (21-56%) in the Bmax values for renal [3H]prazosin, [3H]clonidine and [3H]DHA binding at 1 and 2 weeks following the treatment, without a change in the Kd values. 6-OHDA treatment had no significant effect on the Kd and Bmax values for [3H]QNB binding at 1-3 weeks after the treatment. The norepinephrine (NE) concentration was reduced (68-76%) in the 6-OHDA-treated rat kidney. In conclusion, the present study provides biochemical evidence for the possible localization of postsynaptic α1-, α2- and β-adrenoceptors and muscarinic cholinoceptors in the rat kidney and also for the regulation of these adrenoceptors by the sympathetic nervous system.

Original languageEnglish (US)
Pages (from-to)345-353
Number of pages9
JournalEuropean Journal of Pharmacology
Volume121
Issue number3
DOIs
StatePublished - Mar 4 1986
Externally publishedYes

Keywords

  • Chemical sympathectomy
  • Muscarinic cholinoceptors
  • Norepinephrine
  • Rat kidney
  • α-Adrenoceptors
  • α-Adrenoceptors
  • β-Adrenoceptors

ASJC Scopus subject areas

  • Pharmacology

Fingerprint Dive into the research topics of 'Effects of chemical sympathectomy with 6-hydroxydopamine on α- and β-adrenoceptors and muscarinic cholinoceptors in rat kidney'. Together they form a unique fingerprint.

  • Cite this