Effects of coagulation factor deficiency on plasma coagulation kinetics determined via thrombelastography®: Critical roles of fibrinogen and factors II, VII, X and XII

Vance G Nielsen, B. M. Cohen, E. Cohen

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

Background: Thrombelastography (TEG®) is used to assess coagulopathy. However, a comprehensive characterization of the effects of specific coagulation factor deficiencies and mode of activation on TEG® data does not exist. Methods: Thrombelastography® was performed for 15min with control plasma and plasmas deficient (<1% activity) in Factors II, V, VII, VIII, IX, X, XI, XII, or XIII activated with celite (0.28 mg ml-1) or tissue factor (TF, 0.1%) (n = 6 per condition). Additional fibrinogen concentration activity (75-345 mg dl-1) and Factor II, VII, X and XII activity-response relationships (1%, 6.25%, 12.5%, 25%, 50% and 100% activity) were obtained (n = 8 per condition). Thrombelastography® parameters included reaction time (R), angle (α), and clot strength (A, amplitude; G, elastic modulus). Results: Celite activation of FXII-deficient plasma, TF activation of FVII-deficient and FX-deficient plasma, and celite or TF activation of FII-deficient plasma resulted in an almost undetectable clot. Compared to control values, celite activation of plasmas deficient in FXI, FIX and FVIII resulted in prolonged R and decreased α values, whereas TF activation resulted in decreased α values. Celite and TF activation of FV-deficient plasma resulted in prolonged R and decreased α values, whereas FXIII-deficient plasma had decreased α, A and G-values compared to control values. Conclusions: The fundamental finding of this study is that coagulation factor deficiencies affect TEG® parameters in both a factor-dependent and activation-dependent fashion. Utilizing both celite and TF activation improves the diagnostic power of TEG®. Based on such TEG® data, more targeted administration of blood products could potentially help improve perioperative hemostatic outcomes.

Original languageEnglish (US)
Pages (from-to)222-231
Number of pages10
JournalActa Anaesthesiologica Scandinavica
Volume49
Issue number2
DOIs
StatePublished - Feb 2005
Externally publishedYes

Fingerprint

Thrombelastography
Factor XII
Factor VII
Blood Coagulation Factors
Prothrombin
Diatomaceous Earth
Fibrinogen
Elastic Modulus
Thromboplastin
Hemostatics

Keywords

  • Celite
  • Coagulation factor deficiency
  • Thrombelastography
  • Tissue factor

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

@article{cd570e1922934d0d87c1e6525ea8ac31,
title = "Effects of coagulation factor deficiency on plasma coagulation kinetics determined via thrombelastography{\circledR}: Critical roles of fibrinogen and factors II, VII, X and XII",
abstract = "Background: Thrombelastography (TEG{\circledR}) is used to assess coagulopathy. However, a comprehensive characterization of the effects of specific coagulation factor deficiencies and mode of activation on TEG{\circledR} data does not exist. Methods: Thrombelastography{\circledR} was performed for 15min with control plasma and plasmas deficient (<1{\%} activity) in Factors II, V, VII, VIII, IX, X, XI, XII, or XIII activated with celite (0.28 mg ml-1) or tissue factor (TF, 0.1{\%}) (n = 6 per condition). Additional fibrinogen concentration activity (75-345 mg dl-1) and Factor II, VII, X and XII activity-response relationships (1{\%}, 6.25{\%}, 12.5{\%}, 25{\%}, 50{\%} and 100{\%} activity) were obtained (n = 8 per condition). Thrombelastography{\circledR} parameters included reaction time (R), angle (α), and clot strength (A, amplitude; G, elastic modulus). Results: Celite activation of FXII-deficient plasma, TF activation of FVII-deficient and FX-deficient plasma, and celite or TF activation of FII-deficient plasma resulted in an almost undetectable clot. Compared to control values, celite activation of plasmas deficient in FXI, FIX and FVIII resulted in prolonged R and decreased α values, whereas TF activation resulted in decreased α values. Celite and TF activation of FV-deficient plasma resulted in prolonged R and decreased α values, whereas FXIII-deficient plasma had decreased α, A and G-values compared to control values. Conclusions: The fundamental finding of this study is that coagulation factor deficiencies affect TEG{\circledR} parameters in both a factor-dependent and activation-dependent fashion. Utilizing both celite and TF activation improves the diagnostic power of TEG{\circledR}. Based on such TEG{\circledR} data, more targeted administration of blood products could potentially help improve perioperative hemostatic outcomes.",
keywords = "Celite, Coagulation factor deficiency, Thrombelastography, Tissue factor",
author = "Nielsen, {Vance G} and Cohen, {B. M.} and E. Cohen",
year = "2005",
month = "2",
doi = "10.1111/j.1399-6576.2005.00602.x",
language = "English (US)",
volume = "49",
pages = "222--231",
journal = "Acta Anaesthesiologica Scandinavica",
issn = "0001-5172",
publisher = "Blackwell Munksgaard",
number = "2",

}

TY - JOUR

T1 - Effects of coagulation factor deficiency on plasma coagulation kinetics determined via thrombelastography®

T2 - Critical roles of fibrinogen and factors II, VII, X and XII

AU - Nielsen, Vance G

AU - Cohen, B. M.

AU - Cohen, E.

PY - 2005/2

Y1 - 2005/2

N2 - Background: Thrombelastography (TEG®) is used to assess coagulopathy. However, a comprehensive characterization of the effects of specific coagulation factor deficiencies and mode of activation on TEG® data does not exist. Methods: Thrombelastography® was performed for 15min with control plasma and plasmas deficient (<1% activity) in Factors II, V, VII, VIII, IX, X, XI, XII, or XIII activated with celite (0.28 mg ml-1) or tissue factor (TF, 0.1%) (n = 6 per condition). Additional fibrinogen concentration activity (75-345 mg dl-1) and Factor II, VII, X and XII activity-response relationships (1%, 6.25%, 12.5%, 25%, 50% and 100% activity) were obtained (n = 8 per condition). Thrombelastography® parameters included reaction time (R), angle (α), and clot strength (A, amplitude; G, elastic modulus). Results: Celite activation of FXII-deficient plasma, TF activation of FVII-deficient and FX-deficient plasma, and celite or TF activation of FII-deficient plasma resulted in an almost undetectable clot. Compared to control values, celite activation of plasmas deficient in FXI, FIX and FVIII resulted in prolonged R and decreased α values, whereas TF activation resulted in decreased α values. Celite and TF activation of FV-deficient plasma resulted in prolonged R and decreased α values, whereas FXIII-deficient plasma had decreased α, A and G-values compared to control values. Conclusions: The fundamental finding of this study is that coagulation factor deficiencies affect TEG® parameters in both a factor-dependent and activation-dependent fashion. Utilizing both celite and TF activation improves the diagnostic power of TEG®. Based on such TEG® data, more targeted administration of blood products could potentially help improve perioperative hemostatic outcomes.

AB - Background: Thrombelastography (TEG®) is used to assess coagulopathy. However, a comprehensive characterization of the effects of specific coagulation factor deficiencies and mode of activation on TEG® data does not exist. Methods: Thrombelastography® was performed for 15min with control plasma and plasmas deficient (<1% activity) in Factors II, V, VII, VIII, IX, X, XI, XII, or XIII activated with celite (0.28 mg ml-1) or tissue factor (TF, 0.1%) (n = 6 per condition). Additional fibrinogen concentration activity (75-345 mg dl-1) and Factor II, VII, X and XII activity-response relationships (1%, 6.25%, 12.5%, 25%, 50% and 100% activity) were obtained (n = 8 per condition). Thrombelastography® parameters included reaction time (R), angle (α), and clot strength (A, amplitude; G, elastic modulus). Results: Celite activation of FXII-deficient plasma, TF activation of FVII-deficient and FX-deficient plasma, and celite or TF activation of FII-deficient plasma resulted in an almost undetectable clot. Compared to control values, celite activation of plasmas deficient in FXI, FIX and FVIII resulted in prolonged R and decreased α values, whereas TF activation resulted in decreased α values. Celite and TF activation of FV-deficient plasma resulted in prolonged R and decreased α values, whereas FXIII-deficient plasma had decreased α, A and G-values compared to control values. Conclusions: The fundamental finding of this study is that coagulation factor deficiencies affect TEG® parameters in both a factor-dependent and activation-dependent fashion. Utilizing both celite and TF activation improves the diagnostic power of TEG®. Based on such TEG® data, more targeted administration of blood products could potentially help improve perioperative hemostatic outcomes.

KW - Celite

KW - Coagulation factor deficiency

KW - Thrombelastography

KW - Tissue factor

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U2 - 10.1111/j.1399-6576.2005.00602.x

DO - 10.1111/j.1399-6576.2005.00602.x

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JO - Acta Anaesthesiologica Scandinavica

JF - Acta Anaesthesiologica Scandinavica

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