Effects of cocaine and morphine on IgG production by human peripheral blood lymphocytes in vitro

Francisca Martinez, Ronald R Watson

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Elevated serum levels of IgG are amongst the immunological abnormalities exhibited by intravenous drug addicts. We therefore addressed the hypothesis that cocaine and morphine (the major metabolite of heroin) exert a direct effect on human B cell function in vitro. Human peripheral blood mononuclear cells from normal individuals were incubated for 7 days with the T cell-dependent B cell activator pokeweed mitogen (PWM) and serial dilutions of either cocaine or morphine. At the end of this time total IgG was measured by use of a sandwich ELISA incorporating a biotin-labelled affinity-purified anti-IgG and streptavidin peroxidase. At concentrations relevant to those found in plasma, morphine and cocaine did not affect PWM-stimulated IgG synthesis in vitro. We suggest that these drugs of abuse do not directly influence human B cells, but in vivo exert immune modulatory effects via indirect mechanisms.

Original languageEnglish (US)
JournalLife Sciences
Volume47
Issue number15
DOIs
StatePublished - 1990

Fingerprint

Lymphocytes
Cocaine
Morphine
Pokeweed Mitogens
Blood
B-Lymphocytes
Immunoglobulin G
Cells
Streptavidin
T-cells
Heroin
Street Drugs
Biotin
Metabolites
Drug Users
Peroxidase
Dilution
Blood Cells
Enzyme-Linked Immunosorbent Assay
T-Lymphocytes

ASJC Scopus subject areas

  • Pharmacology

Cite this

Effects of cocaine and morphine on IgG production by human peripheral blood lymphocytes in vitro. / Martinez, Francisca; Watson, Ronald R.

In: Life Sciences, Vol. 47, No. 15, 1990.

Research output: Contribution to journalArticle

@article{176e59acbed54f48bec7be7fbd9976cd,
title = "Effects of cocaine and morphine on IgG production by human peripheral blood lymphocytes in vitro",
abstract = "Elevated serum levels of IgG are amongst the immunological abnormalities exhibited by intravenous drug addicts. We therefore addressed the hypothesis that cocaine and morphine (the major metabolite of heroin) exert a direct effect on human B cell function in vitro. Human peripheral blood mononuclear cells from normal individuals were incubated for 7 days with the T cell-dependent B cell activator pokeweed mitogen (PWM) and serial dilutions of either cocaine or morphine. At the end of this time total IgG was measured by use of a sandwich ELISA incorporating a biotin-labelled affinity-purified anti-IgG and streptavidin peroxidase. At concentrations relevant to those found in plasma, morphine and cocaine did not affect PWM-stimulated IgG synthesis in vitro. We suggest that these drugs of abuse do not directly influence human B cells, but in vivo exert immune modulatory effects via indirect mechanisms.",
author = "Francisca Martinez and Watson, {Ronald R}",
year = "1990",
doi = "10.1016/0024-3205(90)90205-6",
language = "English (US)",
volume = "47",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "15",

}

TY - JOUR

T1 - Effects of cocaine and morphine on IgG production by human peripheral blood lymphocytes in vitro

AU - Martinez, Francisca

AU - Watson, Ronald R

PY - 1990

Y1 - 1990

N2 - Elevated serum levels of IgG are amongst the immunological abnormalities exhibited by intravenous drug addicts. We therefore addressed the hypothesis that cocaine and morphine (the major metabolite of heroin) exert a direct effect on human B cell function in vitro. Human peripheral blood mononuclear cells from normal individuals were incubated for 7 days with the T cell-dependent B cell activator pokeweed mitogen (PWM) and serial dilutions of either cocaine or morphine. At the end of this time total IgG was measured by use of a sandwich ELISA incorporating a biotin-labelled affinity-purified anti-IgG and streptavidin peroxidase. At concentrations relevant to those found in plasma, morphine and cocaine did not affect PWM-stimulated IgG synthesis in vitro. We suggest that these drugs of abuse do not directly influence human B cells, but in vivo exert immune modulatory effects via indirect mechanisms.

AB - Elevated serum levels of IgG are amongst the immunological abnormalities exhibited by intravenous drug addicts. We therefore addressed the hypothesis that cocaine and morphine (the major metabolite of heroin) exert a direct effect on human B cell function in vitro. Human peripheral blood mononuclear cells from normal individuals were incubated for 7 days with the T cell-dependent B cell activator pokeweed mitogen (PWM) and serial dilutions of either cocaine or morphine. At the end of this time total IgG was measured by use of a sandwich ELISA incorporating a biotin-labelled affinity-purified anti-IgG and streptavidin peroxidase. At concentrations relevant to those found in plasma, morphine and cocaine did not affect PWM-stimulated IgG synthesis in vitro. We suggest that these drugs of abuse do not directly influence human B cells, but in vivo exert immune modulatory effects via indirect mechanisms.

UR - http://www.scopus.com/inward/record.url?scp=0025052462&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025052462&partnerID=8YFLogxK

U2 - 10.1016/0024-3205(90)90205-6

DO - 10.1016/0024-3205(90)90205-6

M3 - Article

C2 - 2233132

AN - SCOPUS:0025052462

VL - 47

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

IS - 15

ER -