Effects of continuous inhalation of dichloromethane in the mouse

Morphologic and functional observations

Ronald S Weinstein, Dale D. Boyd, Kenneth C. Back

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Continuous inhalation of 5000 ppm CH2Cl2 caused balloon degeneration, transient severe fatty change and partial inhibition of leucine incorporation into liver proteins of 20-32 g female mice of the ICR stain. The earliest lesion was identified at 12 hr of exposure and consisted of dissociation of polyribosomes and swelling of hepatocyte rough endoplasmic reticulum (so-called "balloon" degeneration). Balloon degeneration peaked in severity at 2 days of exposure and then partially reversed. Liver fatty change was also partially reversible. There was a 12-fold increase in liver triglycerides with 3 days of exposure, but at 1 wk of continuous exposure liver triglycerides were two to three times control values. Necrosis was observed in a few isolated hepatocytes. The reversibility of the liver changes suggests that partial tolerance develops in hepatocytes with continuous exposure to 5000 ppm dichloromethane. Liver lesions produced in the mouse liver by dichloromethane bear some resemblance to the lesions of carbon tetrachloride hepatotoxicity but appear to be less severe.

Original languageEnglish (US)
Pages (from-to)660-679
Number of pages20
JournalToxicology and Applied Pharmacology
Volume23
Issue number4
DOIs
StatePublished - 1972
Externally publishedYes

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Methylene Chloride
Liver
Inhalation
Balloons
Hepatocytes
Triglycerides
Inbred ICR Mouse
Polyribosomes
Carbon Tetrachloride
Rough Endoplasmic Reticulum
Fatty Liver
Leucine
Necrosis
Coloring Agents
Swelling
Proteins

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Effects of continuous inhalation of dichloromethane in the mouse : Morphologic and functional observations. / Weinstein, Ronald S; Boyd, Dale D.; Back, Kenneth C.

In: Toxicology and Applied Pharmacology, Vol. 23, No. 4, 1972, p. 660-679.

Research output: Contribution to journalArticle

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