Effects of denervation on spectrin concentration in avian skeletal muscle

Carol Gregorio, M. S. Hudecki, C. M. Pollina, E. A. Repasky

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The effect of denervation on avian muscle α-spectrin was examined in fast and slow muscles. Using immunofluorescence, the surgically denervated fast-twitch posterior latissimus forsi (PLD) exhibited a significant increase in spectrin antigen associated with the sarcolemma and within the sarcoplasm compared with the contralateral innervated control muscle. Using gel electrophoresis followed by immunoblotting, we found a two- to three-fold increase in the levels of spectrin in the denervated PLD over that found in the innervated PLD. These levels were comparable to those found previously in slow and dystrophic muscle. The intrafiber distribution of spectrin is similar between the denervated PLD and the slow-tonic anterior latissimus dorsi (ALD). When spectrin was examined in dystrophic PLD muscle, denervation was found to have no effect. These results support our hypothesis that the concentration of spectrin within muscle fibers reflects the physiological state of those fibers. Changes in spectrin concentration may be a useful probe to study the various alterations in physical parameters found among fast, slow, dystrophic, and denervated fibers.

Original languageEnglish (US)
Pages (from-to)372-380
Number of pages9
JournalMuscle and Nerve
Volume11
Issue number4
StatePublished - 1988
Externally publishedYes

Fingerprint

Spectrin
Denervation
Skeletal Muscle
Muscles
Muscle Denervation
Sarcolemma
Superficial Back Muscles
Immunoblotting
Fluorescent Antibody Technique
Electrophoresis
Gels
1-dodecylpyridoxal
Antigens

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Gregorio, C., Hudecki, M. S., Pollina, C. M., & Repasky, E. A. (1988). Effects of denervation on spectrin concentration in avian skeletal muscle. Muscle and Nerve, 11(4), 372-380.

Effects of denervation on spectrin concentration in avian skeletal muscle. / Gregorio, Carol; Hudecki, M. S.; Pollina, C. M.; Repasky, E. A.

In: Muscle and Nerve, Vol. 11, No. 4, 1988, p. 372-380.

Research output: Contribution to journalArticle

Gregorio, C, Hudecki, MS, Pollina, CM & Repasky, EA 1988, 'Effects of denervation on spectrin concentration in avian skeletal muscle', Muscle and Nerve, vol. 11, no. 4, pp. 372-380.
Gregorio, Carol ; Hudecki, M. S. ; Pollina, C. M. ; Repasky, E. A. / Effects of denervation on spectrin concentration in avian skeletal muscle. In: Muscle and Nerve. 1988 ; Vol. 11, No. 4. pp. 372-380.
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