Effects of dicyclohexylamine sulfate, a spermidine synthase inhibitor, in 9L rat brain tumor cells

Burt G.F. Feuerstein, D. F. Deen, L. J. Marton

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Abstract

Growth characteristics, polyamine levels, and distribution of cells in the cell cycle were determined for 9L rat brain tumor cells treated for various periods with 1 mM dicyclohexylamine sulfate (DCHA). Continuous treatment of cells with DCHA caused growth inhibition at 2 days of treatment. After 2 days of treatment the growth rate of cells increased to approximately the same rate as control cells, even though treatment was continuous. Levels of spermidine were depleted to less than 10% of control levels, spermine levels were essentially unchanged, and putrescine levels were elevated to more than 350% of control levels after 9L cells were treated with DCHA for 2 days. In contrast to results found for the polyamine biosynthesis inhibitor α-difluoromethylornithine, treatment of 9L cells with DCHA did not potentiate the cytotoxicity of 1,3-bis(2-chloroethyl)-1-nitrosourea. To mimic the effects on polyamine levels caused by treatment with DCHA, 9L cells were treated with 5 mM putrescine alone or with 5 mM putrescine and 1 mM DCHA after treatment with 1 mM α-difluoromethylornithine. Results of these experiments suggest that treatment with DCHA alone does not potentiate the cytotoxicity of 1,3-bis(2-chloroethyl)-1-nitrosourea because elevated levels of putrescine caused by treatment counteract the effects of decreased spermidine levels.

Original languageEnglish (US)
Pages (from-to)4950-4954
Number of pages5
JournalCancer Research
Volume45
Issue number10
StatePublished - 1985
Externally publishedYes

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Spermidine Synthase
Brain Neoplasms
Putrescine
Polyamines
Eflornithine
Carmustine
Spermidine
Growth
Spermine
dicyclohexylamine
Cell Cycle

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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Effects of dicyclohexylamine sulfate, a spermidine synthase inhibitor, in 9L rat brain tumor cells. / Feuerstein, Burt G.F.; Deen, D. F.; Marton, L. J.

In: Cancer Research, Vol. 45, No. 10, 1985, p. 4950-4954.

Research output: Contribution to journalArticle

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