Effects of exercise training and ACE inhibition on insulin action in rat skeletal muscle

Kara R. Foianini, Michelle S. Steen, Tyson R. Kinnick, Melanie B. Schmit, Erik B. Youngblood, Erik J Henriksen

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Our laboratory has demonstrated (Steen MS, Foianini KR, Youngblood EB, Kinnick TR, Jacob S, and Henriksen EJ, J Appl Physiol 86: 2044-2051, 1999) that exercise training and treatment with the angiotensin-converting enzyme (ACE) inhibitor trandolapril interact to improve insulin action in insulin-resistant obese Zucker rats. The present study was undertaken to determine whether a similar interactive effect of these interventions is manifest in an animal model of normal insulin sensitivity. Lean Zucker (Fa/-) rats were assigned to either a sedentary, trandolapril-treated (1 mg · kg-1 · day-1 for 6 wk), exercise-trained (treadmill running for 6 wk), or combined trandolapril-treated and exercise-trained group. Exercise training alone or in combination with trandolapril significantly (P < 0.05) increased peak oxygen consumption by 26-32%. Compared with sedentary controls, exercise training alone or in combination with ACE inhibitor caused smaller areas under the curve for glucose (27-37%) and insulin (41-44%) responses during an oral glucose tolerance test. Exercise training alone or in combination with trandolapril also improved insulin-stimulated glucose transport in isolated epitrochlearis (33-50%) and soleus (58-66%) muscles. The increases due to exercise training alone or in combination with trandolapril were associated with enhanced muscle GLUT-4 protein levels and total hexokinase activities. However, there was no interactive effect of exercise training and ACE inhibition observed on insulin action. These results indicate that, in rats with normal insulin sensitivity, exercise training improves oral glucose tolerance and insulin-stimulated muscle glucose transport, whereas ACE inhibition has no effect. Moreover, the beneficial interactive effects of exercise training and ACE inhibition on these parameters are not apparent in lean Zucker rats and, therefore, are restricted to conditions of insulin resistance.

Original languageEnglish (US)
Pages (from-to)687-694
Number of pages8
JournalJournal of Applied Physiology
Volume89
Issue number2
StatePublished - 2000

Fingerprint

trandolapril
Peptidyl-Dipeptidase A
Skeletal Muscle
Exercise
Insulin
Zucker Rats
Insulin Resistance
Glucose Tolerance Test
Angiotensin-Converting Enzyme Inhibitors
Glucose
Glucose Transporter Type 4
Muscles
Hexokinase
Muscle Proteins
Oxygen Consumption
Running
Area Under Curve
Animal Models

Keywords

  • Angiotensin-converting enzyme
  • Glucose transport
  • GLUT-4 protein
  • Lean Zucker rat
  • Treadmill running

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Foianini, K. R., Steen, M. S., Kinnick, T. R., Schmit, M. B., Youngblood, E. B., & Henriksen, E. J. (2000). Effects of exercise training and ACE inhibition on insulin action in rat skeletal muscle. Journal of Applied Physiology, 89(2), 687-694.

Effects of exercise training and ACE inhibition on insulin action in rat skeletal muscle. / Foianini, Kara R.; Steen, Michelle S.; Kinnick, Tyson R.; Schmit, Melanie B.; Youngblood, Erik B.; Henriksen, Erik J.

In: Journal of Applied Physiology, Vol. 89, No. 2, 2000, p. 687-694.

Research output: Contribution to journalArticle

Foianini, KR, Steen, MS, Kinnick, TR, Schmit, MB, Youngblood, EB & Henriksen, EJ 2000, 'Effects of exercise training and ACE inhibition on insulin action in rat skeletal muscle', Journal of Applied Physiology, vol. 89, no. 2, pp. 687-694.
Foianini, Kara R. ; Steen, Michelle S. ; Kinnick, Tyson R. ; Schmit, Melanie B. ; Youngblood, Erik B. ; Henriksen, Erik J. / Effects of exercise training and ACE inhibition on insulin action in rat skeletal muscle. In: Journal of Applied Physiology. 2000 ; Vol. 89, No. 2. pp. 687-694.
@article{8c40df9b94324311b0d3b432cb57a340,
title = "Effects of exercise training and ACE inhibition on insulin action in rat skeletal muscle",
abstract = "Our laboratory has demonstrated (Steen MS, Foianini KR, Youngblood EB, Kinnick TR, Jacob S, and Henriksen EJ, J Appl Physiol 86: 2044-2051, 1999) that exercise training and treatment with the angiotensin-converting enzyme (ACE) inhibitor trandolapril interact to improve insulin action in insulin-resistant obese Zucker rats. The present study was undertaken to determine whether a similar interactive effect of these interventions is manifest in an animal model of normal insulin sensitivity. Lean Zucker (Fa/-) rats were assigned to either a sedentary, trandolapril-treated (1 mg · kg-1 · day-1 for 6 wk), exercise-trained (treadmill running for 6 wk), or combined trandolapril-treated and exercise-trained group. Exercise training alone or in combination with trandolapril significantly (P < 0.05) increased peak oxygen consumption by 26-32{\%}. Compared with sedentary controls, exercise training alone or in combination with ACE inhibitor caused smaller areas under the curve for glucose (27-37{\%}) and insulin (41-44{\%}) responses during an oral glucose tolerance test. Exercise training alone or in combination with trandolapril also improved insulin-stimulated glucose transport in isolated epitrochlearis (33-50{\%}) and soleus (58-66{\%}) muscles. The increases due to exercise training alone or in combination with trandolapril were associated with enhanced muscle GLUT-4 protein levels and total hexokinase activities. However, there was no interactive effect of exercise training and ACE inhibition observed on insulin action. These results indicate that, in rats with normal insulin sensitivity, exercise training improves oral glucose tolerance and insulin-stimulated muscle glucose transport, whereas ACE inhibition has no effect. Moreover, the beneficial interactive effects of exercise training and ACE inhibition on these parameters are not apparent in lean Zucker rats and, therefore, are restricted to conditions of insulin resistance.",
keywords = "Angiotensin-converting enzyme, Glucose transport, GLUT-4 protein, Lean Zucker rat, Treadmill running",
author = "Foianini, {Kara R.} and Steen, {Michelle S.} and Kinnick, {Tyson R.} and Schmit, {Melanie B.} and Youngblood, {Erik B.} and Henriksen, {Erik J}",
year = "2000",
language = "English (US)",
volume = "89",
pages = "687--694",
journal = "Journal of Applied Physiology",
issn = "8750-7587",
publisher = "American Physiological Society",
number = "2",

}

TY - JOUR

T1 - Effects of exercise training and ACE inhibition on insulin action in rat skeletal muscle

AU - Foianini, Kara R.

AU - Steen, Michelle S.

AU - Kinnick, Tyson R.

AU - Schmit, Melanie B.

AU - Youngblood, Erik B.

AU - Henriksen, Erik J

PY - 2000

Y1 - 2000

N2 - Our laboratory has demonstrated (Steen MS, Foianini KR, Youngblood EB, Kinnick TR, Jacob S, and Henriksen EJ, J Appl Physiol 86: 2044-2051, 1999) that exercise training and treatment with the angiotensin-converting enzyme (ACE) inhibitor trandolapril interact to improve insulin action in insulin-resistant obese Zucker rats. The present study was undertaken to determine whether a similar interactive effect of these interventions is manifest in an animal model of normal insulin sensitivity. Lean Zucker (Fa/-) rats were assigned to either a sedentary, trandolapril-treated (1 mg · kg-1 · day-1 for 6 wk), exercise-trained (treadmill running for 6 wk), or combined trandolapril-treated and exercise-trained group. Exercise training alone or in combination with trandolapril significantly (P < 0.05) increased peak oxygen consumption by 26-32%. Compared with sedentary controls, exercise training alone or in combination with ACE inhibitor caused smaller areas under the curve for glucose (27-37%) and insulin (41-44%) responses during an oral glucose tolerance test. Exercise training alone or in combination with trandolapril also improved insulin-stimulated glucose transport in isolated epitrochlearis (33-50%) and soleus (58-66%) muscles. The increases due to exercise training alone or in combination with trandolapril were associated with enhanced muscle GLUT-4 protein levels and total hexokinase activities. However, there was no interactive effect of exercise training and ACE inhibition observed on insulin action. These results indicate that, in rats with normal insulin sensitivity, exercise training improves oral glucose tolerance and insulin-stimulated muscle glucose transport, whereas ACE inhibition has no effect. Moreover, the beneficial interactive effects of exercise training and ACE inhibition on these parameters are not apparent in lean Zucker rats and, therefore, are restricted to conditions of insulin resistance.

AB - Our laboratory has demonstrated (Steen MS, Foianini KR, Youngblood EB, Kinnick TR, Jacob S, and Henriksen EJ, J Appl Physiol 86: 2044-2051, 1999) that exercise training and treatment with the angiotensin-converting enzyme (ACE) inhibitor trandolapril interact to improve insulin action in insulin-resistant obese Zucker rats. The present study was undertaken to determine whether a similar interactive effect of these interventions is manifest in an animal model of normal insulin sensitivity. Lean Zucker (Fa/-) rats were assigned to either a sedentary, trandolapril-treated (1 mg · kg-1 · day-1 for 6 wk), exercise-trained (treadmill running for 6 wk), or combined trandolapril-treated and exercise-trained group. Exercise training alone or in combination with trandolapril significantly (P < 0.05) increased peak oxygen consumption by 26-32%. Compared with sedentary controls, exercise training alone or in combination with ACE inhibitor caused smaller areas under the curve for glucose (27-37%) and insulin (41-44%) responses during an oral glucose tolerance test. Exercise training alone or in combination with trandolapril also improved insulin-stimulated glucose transport in isolated epitrochlearis (33-50%) and soleus (58-66%) muscles. The increases due to exercise training alone or in combination with trandolapril were associated with enhanced muscle GLUT-4 protein levels and total hexokinase activities. However, there was no interactive effect of exercise training and ACE inhibition observed on insulin action. These results indicate that, in rats with normal insulin sensitivity, exercise training improves oral glucose tolerance and insulin-stimulated muscle glucose transport, whereas ACE inhibition has no effect. Moreover, the beneficial interactive effects of exercise training and ACE inhibition on these parameters are not apparent in lean Zucker rats and, therefore, are restricted to conditions of insulin resistance.

KW - Angiotensin-converting enzyme

KW - Glucose transport

KW - GLUT-4 protein

KW - Lean Zucker rat

KW - Treadmill running

UR - http://www.scopus.com/inward/record.url?scp=0033871543&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033871543&partnerID=8YFLogxK

M3 - Article

C2 - 10926655

AN - SCOPUS:0033871543

VL - 89

SP - 687

EP - 694

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 8750-7587

IS - 2

ER -