Effects of furosemide on renal calcium handling

Chien Te Lee, Hung Chun Chen, Li-Wen Lai, Kim Chong Yong, Yeong Hau H Lien

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Furosemide is a loop diuretic agent that has been used to treat hypercalcemia because it increases renal calcium excretion. The effect of furosemide on calcium transport molecules in distal tubules has yet to be investigated. We conducted studies to examine the effects of furosemide on renal calcium excretion and expression of calcium transport molecules in mice. Mice were administered with a single dose of furosemide (15 mg/kg) and examined 4 h later or were given twice-daily furosemide injections for 3 days. To evaluate the effects of volume depletion, drinking water was supplemented with salt. Our results showed that, in acute experiments, furosemide enhanced urinary calcium excretion, which was associated with a significant increase in mRNA levels of TRPV5, TRPV6, and calbindin-D28k but not calbindin-D9k as measured by real-time PCR (TRPV5 and TRPV6 are transient receptor potential vanilloid 5 and 6). Chronic furosemide administration induced three-to fourfold increases in urinary calcium excretion and elevated mRNA levels of TRPV5, TRPV6, calbindin-D28k, and calbindin-D9k without or with salt supplement. Similar upregulation of calcium transport molecules was observed in mice with gentamicin-induced hypercalciuria. Coadministration of chlorothiazide decreased furosemide-induced calciuria, either acutely or chronically, although still accompanied by upregulation of these transport molecules. Immunofluorescent staining studies revealed comparably increased protein abundance in TRPV5 and calbindin-D28k. We conclude that furosemide treatment enhances urinary calcium excretion. Increased abundance of calcium transport molecules in the distal convoluted tubule represents a solute load-dependent effect in response to increased calcium delivery and serves as a compensatory adaptation in the downstream segment.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume293
Issue number4
DOIs
StatePublished - Oct 2007

Fingerprint

Furosemide
Calcium
Kidney
Calbindin 1
Calbindins
Up-Regulation
Salts
Chlorothiazide
Sodium Potassium Chloride Symporter Inhibitors
TRPV Cation Channels
Hypercalciuria
Messenger RNA
Hypercalcemia
Gentamicins
Drinking Water
Real-Time Polymerase Chain Reaction
Staining and Labeling
Injections

Keywords

  • Calbindin-D28k
  • Calbindin-D9k
  • Thiazides
  • TRPV5
  • TRPV6

ASJC Scopus subject areas

  • Physiology

Cite this

Effects of furosemide on renal calcium handling. / Lee, Chien Te; Chen, Hung Chun; Lai, Li-Wen; Yong, Kim Chong; Lien, Yeong Hau H.

In: American Journal of Physiology - Renal Physiology, Vol. 293, No. 4, 10.2007.

Research output: Contribution to journalArticle

Lee, Chien Te ; Chen, Hung Chun ; Lai, Li-Wen ; Yong, Kim Chong ; Lien, Yeong Hau H. / Effects of furosemide on renal calcium handling. In: American Journal of Physiology - Renal Physiology. 2007 ; Vol. 293, No. 4.
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