Effects of gender on severity and pulmonary artery vascular reactivity in chronic hypoxic pulmonary hypertension in mice

Jun Wan, Jason X.J. Yuan, Ran Miao, Zhenguo Zhai, Mingyuan Zhang, Yuanhua Yang, Jun Wang, Chen Wang

Research output: Contribution to journalArticle

Abstract

Objective: To explore the effects of gender on pathology and pulmonary vascular reactivity in chronic hypoxic pulmonary hypertension (HPH) in mice. Methods: HPH was induced by a 4-week exposure of male and female mice (8 weeks old C57BL/6, n=15 each) to chronic hypoxia (10%O2). Normoxic controls were established both in male and female groups (n=15 each). Right ventricular systolic pressure (RVSP) and Fulton index were detected. Isolated pulmonary artery ring contraction induced by high potassium (K+) and U-46619 was recorded and compared between two groups. Results: Chronic hypoxia induced pulmonary hypertension after 4 weeks. RVSP significantly increased both in hypoxic female and male groups (normoxic vs hypoxic: (23.40 ± 0.16) vs (31.46 ± 2.33) and (24.71 ± 0.44) vs (33.66 ± 1.38) mmHg (1 mmHg=0.133 kPa), P=0.029 and P < 0.001). However, there were no inter-group differences (P=0.447). The Fulton index of hypoxic female and male mice was higher than normoxic counterparts (P=0.001, P < 0.001). But there were no gender differences (P=0.471). Contraction of high K+ and U-46619 was calcium ion (Ca2+)-dependent and decreased markedly without calcium (P < 0.001, P=0.007). High K+ and U-46619-induced contraction of pulmonary artery was concentration dependent and greater in chronic hypoxic female (P=0.002, P < 0.001) and male mice (both P < 0.001). But no differences existed between male and female mice in high K+ (P =0.657) or U-46619 (P=0.751) -induced contraction. Conclusion: No significant correlation exists between gender and occurrence and severity of pulmonary hypertension or pulmonary artery vascular reactivity in HPH mice.

Original languageEnglish (US)
Pages (from-to)1692-1695
Number of pages4
JournalNational Medical Journal of China
Volume94
Issue number22
DOIs
StatePublished - Jun 10 2014
Externally publishedYes

Keywords

  • Anoxia
  • Gender identity
  • Hypertension, pulmonary
  • Mice
  • Vascular reactivity

ASJC Scopus subject areas

  • Medicine(all)

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