Effects of halothane and other volatile anaesthetics on protein synthesis and secretion in guineapig liver slices

H. N. Ghantous, J. L. Fernando, R. L. Keith, A. J. Gandolfi, K. Brendel

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Summary: We have investigated the effect of volatile anaesthetics on protein synthesis and secretion in Hartley male guineapig liver slices. The slices (250-300 μm thick) were incubated in sealed roller vials containing Krebs-Henseleit buffer at 37°C under 95% oxygen. Volatile anaesthetics were vaporized in the vials to produce constant concentrations in the medium. Halothane 1-2.1 mmol litre-1 produced a concentration-related decrease in protein synthesis (3H-leucine incorporation) and secretion. Deuter-ated halothane (d-halothane), which is less bio-transformed, was less inhibiting than halothane: uptake of the 3H-leucine was not affected but its incorporation into the nascent peptide was inhibited. Enflurane 2.2 mmol litre-1, isoflurane 2.2 mmol litre-1 and sevoflurane 2.1 mmol litre-1 also inhibited protein synthesis, but to a lesser extent than halothane and d-halothane. We conclude that alterations in protein synthesis and secretion are an early and sensitive indicator of cellular injury by volatile anaesthetics in liver slices.

Original languageEnglish (US)
Pages (from-to)172-177
Number of pages6
JournalBritish Journal of Anaesthesia
Volume68
Issue number2
DOIs
StatePublished - Feb 1992

Keywords

  • Anaesthetics, volatile: enflurane, halothane, isoflurane, sevoflurane
  • Complications: hepatotoxicity
  • Liver: protein synthesis

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

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