Effects of heat stress and nutrition on lactating Holstein cows

II. Aspects of hepatic growth hormone responsiveness

M. L. Rhoads, J. W. Kim, Robert J Collier, B. A. Crooker, Y. R. Boisclair, L. H. Baumgard, R. P. Rhoads

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Heat stress (HS) is a multibillion-dollar problem for the global dairy industry, and reduced milk yield is the primary contributor to this annual economic loss. Feed intake declines precipitously during HS but accounts for only about 35% of the decreased milk synthesis, indicating that the physiological mechanisms responsible for decreased milk production during HS are only partly understood. Thus, our experimental objectives were to characterize the direct effects of HS on the somatotropic axis, a primary regulator of metabolism and milk yield. We recently reported no differences in mean growth hormone (GH) concentrations, GH pulsatility characteristics, or GH response to growth hormone releasing factor in HS versus pair-fed (PF) thermoneutral controls. Despite similarities in circulating GH characteristics, plasma insulin-like growth factor (IGF)-I concentrations were reduced during heat stress conditions but not in PF animals, suggesting that uncoupling of the hepatic GH-IGF axis may occur during HS. We investigated this possibility by measuring proximal indicators of hepatic GH signaling following a GH bolus. Heat stress but not PF decreased abundance of the GH receptor and GH-dependent signal transducer and activator of transcription (STAT)-5 phosphorylation. Consistent with reduced GH signaling through STAT-5, basal hepatic IGF-I mRNA abundance was lower in HS cows. Thus, the reduced hepatic GH responsiveness (in terms of IGF-I gene expression) observed during HS appears to involve mechanisms at least partially independent of reduced nutrient intake. The physiological significance of reduced hepatic GH receptor abundance during HS is unclear at this time. Aside from reducing IGF-I production, it may reduce other GH-sensitive bioenergetic processes such as gluconeogenesis.

Original languageEnglish (US)
Pages (from-to)170-179
Number of pages10
JournalJournal of Dairy Science
Volume93
Issue number1
DOIs
StatePublished - Jan 2010

Fingerprint

somatotropin
Growth Hormone
heat stress
Holstein
Hot Temperature
nutrition
cows
liver
Liver
insulin-like growth factor I
Insulin-Like Growth Factor I
Milk
STAT5 Transcription Factor
Somatotropin Receptors
milk yield
Dairying
transcription (genetics)
somatoliberin
milk synthesis
Growth Hormone-Releasing Hormone

Keywords

  • Heat stress
  • Hyperthermia
  • Somatotropin

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Food Science
  • Genetics

Cite this

Effects of heat stress and nutrition on lactating Holstein cows : II. Aspects of hepatic growth hormone responsiveness. / Rhoads, M. L.; Kim, J. W.; Collier, Robert J; Crooker, B. A.; Boisclair, Y. R.; Baumgard, L. H.; Rhoads, R. P.

In: Journal of Dairy Science, Vol. 93, No. 1, 01.2010, p. 170-179.

Research output: Contribution to journalArticle

Rhoads, M. L. ; Kim, J. W. ; Collier, Robert J ; Crooker, B. A. ; Boisclair, Y. R. ; Baumgard, L. H. ; Rhoads, R. P. / Effects of heat stress and nutrition on lactating Holstein cows : II. Aspects of hepatic growth hormone responsiveness. In: Journal of Dairy Science. 2010 ; Vol. 93, No. 1. pp. 170-179.
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