Abstract
Tat protein of HIV-1 is a potent transactivator of transcription and essential for HIV-1 replication. In addition, Tat has been proposed to possess immunosuppressive functions, suggesting that Tat may play a direct role in the immune dysfunction associated with AIDS. Recently, it has been reported that Tat represses activity of a major histocompatibility complex (MHC) class I gene promoter. Because HIV infection downmodulates expression of class I molecules, this data strongly suggests that Tat downregulates class I expression and leads to loss of CTL activity. Here, we report effects of Tat on class I expression using a human cell line, T0, expressing Tat (T0- Tat). Northern blot analysis shows that levels of MHC class I transcripts are normal in T0-Tat. Flow cytometry analyses indicate that expression of HLA class I molecules is not substantially downregulated to any great extent by Tat in T0-Tat. Further, pulse-chase experiments followed by Endoglycosidase- H treatment show that the rate of maturation and processing of class I molecules in T0-Tat is indistinguishable from that in the original cell line, T0. Taken together, these data suggest that Tat expression does not necessarily result in downregulation of class I expression.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 233-240 |
| Number of pages | 8 |
| Journal | Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology |
| Volume | 11 |
| Issue number | 3 |
| State | Published - 1996 |
| Externally published | Yes |
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Keywords
- HIV-1
- Immunosuppression
- MHC class I molecule
- Tat
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Virology
Cite this
Effects of HIV-1 Tat on expression of HLA class I molecules. / Matsui, Masanori; Warburton, Robert J.; Cogswell, Patricia C.; Baldwin, Albert S.; Frelinger, Jeffrey A.
In: Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology, Vol. 11, No. 3, 1996, p. 233-240.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of HIV-1 Tat on expression of HLA class I molecules
AU - Matsui, Masanori
AU - Warburton, Robert J.
AU - Cogswell, Patricia C.
AU - Baldwin, Albert S.
AU - Frelinger, Jeffrey A
PY - 1996
Y1 - 1996
N2 - Tat protein of HIV-1 is a potent transactivator of transcription and essential for HIV-1 replication. In addition, Tat has been proposed to possess immunosuppressive functions, suggesting that Tat may play a direct role in the immune dysfunction associated with AIDS. Recently, it has been reported that Tat represses activity of a major histocompatibility complex (MHC) class I gene promoter. Because HIV infection downmodulates expression of class I molecules, this data strongly suggests that Tat downregulates class I expression and leads to loss of CTL activity. Here, we report effects of Tat on class I expression using a human cell line, T0, expressing Tat (T0- Tat). Northern blot analysis shows that levels of MHC class I transcripts are normal in T0-Tat. Flow cytometry analyses indicate that expression of HLA class I molecules is not substantially downregulated to any great extent by Tat in T0-Tat. Further, pulse-chase experiments followed by Endoglycosidase- H treatment show that the rate of maturation and processing of class I molecules in T0-Tat is indistinguishable from that in the original cell line, T0. Taken together, these data suggest that Tat expression does not necessarily result in downregulation of class I expression.
AB - Tat protein of HIV-1 is a potent transactivator of transcription and essential for HIV-1 replication. In addition, Tat has been proposed to possess immunosuppressive functions, suggesting that Tat may play a direct role in the immune dysfunction associated with AIDS. Recently, it has been reported that Tat represses activity of a major histocompatibility complex (MHC) class I gene promoter. Because HIV infection downmodulates expression of class I molecules, this data strongly suggests that Tat downregulates class I expression and leads to loss of CTL activity. Here, we report effects of Tat on class I expression using a human cell line, T0, expressing Tat (T0- Tat). Northern blot analysis shows that levels of MHC class I transcripts are normal in T0-Tat. Flow cytometry analyses indicate that expression of HLA class I molecules is not substantially downregulated to any great extent by Tat in T0-Tat. Further, pulse-chase experiments followed by Endoglycosidase- H treatment show that the rate of maturation and processing of class I molecules in T0-Tat is indistinguishable from that in the original cell line, T0. Taken together, these data suggest that Tat expression does not necessarily result in downregulation of class I expression.
KW - HIV-1
KW - Immunosuppression
KW - MHC class I molecule
KW - Tat
UR - http://www.scopus.com/inward/record.url?scp=0029963033&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029963033&partnerID=8YFLogxK
M3 - Article
C2 - 8603259
AN - SCOPUS:0029963033
VL - 11
SP - 233
EP - 240
JO - Journal of Acquired Immune Deficiency Syndromes
JF - Journal of Acquired Immune Deficiency Syndromes
SN - 1525-4135
IS - 3
ER -