Effects of H2O2 on insulin signaling the glucose transport system in mammalian skeletal muscle

Research output: Chapter in Book/Report/Conference proceedingChapter

12 Citations (Scopus)

Abstract

Hydrogen peroxide (H2O2) is an important regulator of cellular events leading to glucose transport activation in mammalian skeletal muscle. In the absence of insulin, H2O2 in the low micromolar range engages the canonical IRS-1/PI3K/Akt-dependent insulin signaling pathway, as well as other signaling elements (AMPK and p38 MAPK), to increase basal glucose transport activity. In contrast, in the presence of insulin, H2O2 antagonizes insulin signaling by recruitment of various deleterious serine/threonine kinases, producing a state of insulin resistance. Here, we describe the H2O2 enzymatic-generating system, utilizing glucose oxidase, that has been used to investigate the impact of H2O2 on cellular signaling mechanisms related to glucose transport activity in isolated rat skeletal muscle preparations, such as the soleus. By varying the glucose oxidase concentration in the medium, target ranges of steady-state H2O2 concentrations (30-90 μM) can be attained for up to 6 h, with subsequent assessment of cellular signaling and glucose transport activity.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
Pages269-278
Number of pages10
Volume528
DOIs
StatePublished - 2013

Publication series

NameMethods in Enzymology
Volume528
ISSN (Print)00766879
ISSN (Electronic)15577988

Fingerprint

Muscle
Skeletal Muscle
Insulin
Glucose
Glucose Oxidase
Cell signaling
AMP-Activated Protein Kinases
Protein-Serine-Threonine Kinases
p38 Mitogen-Activated Protein Kinases
Phosphatidylinositol 3-Kinases
Hydrogen Peroxide
Insulin Resistance
Rats
Chemical activation

Keywords

  • HO enzymatic-generating system
  • In vitro
  • Insulin resistance
  • Oxidant stress
  • Soleus muscle

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Medicine(all)

Cite this

Effects of H2O2 on insulin signaling the glucose transport system in mammalian skeletal muscle. / Henriksen, Erik J.

Methods in Enzymology. Vol. 528 2013. p. 269-278 (Methods in Enzymology; Vol. 528).

Research output: Chapter in Book/Report/Conference proceedingChapter

Henriksen, Erik J. / Effects of H2O2 on insulin signaling the glucose transport system in mammalian skeletal muscle. Methods in Enzymology. Vol. 528 2013. pp. 269-278 (Methods in Enzymology).
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