Effects of implementing PSI-light on hydrogen production via biophotolysis in Chlamydomonas reinhardtii mutant strains

Takanori Hoshino, Daniel J. Johnson, Matthew Scholz, Joel L. Cuello

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

A new strategy in hydrogen production via biophotolysis developed previously was implemented in mutant strains of Chlamydomonas reinhardtii. Implementing PSI-light successfully demonstrated improved hydrogen production in the wild type strain of C. reinhardtii in a previous study, however, the results also showed rapid attenuation of hydrogen production even under PSI-light due to inhibited hydrogenase activity caused by oxygen, which was simultaneously produced through the water splitting reactions of PSII under light. In order to further decrease oxygen generation under PSI-light during the hydrogen production phase, use of some mutant strains of C. reinhardtii, that are known to show limited oxygen generation, were investigated.Continuous supply of PSI-light after a short anaerobic adaptation under dark conditions achieved significantly prolonged hydrogen production up to ≈ 18h in a chlorophyll b deficient mutant (cbn 1-48) and a very high light tolerant mutant (VHLR-S4) yielding chlorophyll content based H2 production of 220 and 176dm3kg-1 (equivalent to dry cell weight based H2 production of 4.24 and 8.73dm3kg-1), respectively. In addition, by iterating light and dark every 1.5h with PSI-light, hydrogen production was successfully extended to 27h yielding chlorophyll content based H2 production of 366dm3kg-1 (equivalent to dry cell weight based H2 production of 8.81dm3kg-1) in cbn 1-48. Further, greater energy conversion efficiency from light to the formation of hydrogen molecules was achieved with the combination of PSI-light and some mutant strains compared to alternate methods of biophotolysis.

Original languageEnglish (US)
Pages (from-to)243-252
Number of pages10
JournalBiomass and Bioenergy
Volume59
DOIs
StatePublished - Dec 1 2013

Keywords

  • Algae
  • Biofuel
  • Chlamydomonas reinhardtii
  • Hydrogen
  • LEDs
  • Photosystem

ASJC Scopus subject areas

  • Forestry
  • Renewable Energy, Sustainability and the Environment
  • Agronomy and Crop Science
  • Waste Management and Disposal

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