Effects of incubations in low potassium and low sodium media on Na-K-ATPase activity in snake and chicken kidney slices

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Abstract

1. 1. Effects of incubating snake and chicken kidney slices in media of varying potassium and sodium concentrations on the sodium-potassium-activated adenosine triphosphatase (Na-K-ATPase) activity of these slices were evaluated. 2. 2. Prolonged (2-hr) incubation of chicken kidney slices in potassium-free medium produced significant residual depression in Na-K-ATPase activity in heavy microsomes. Similar incubation of snake kidney slices in potassium-free medium had no residual effect on Na-K-ATPase activity. 3. 3. When potassium-depleted chicken kidney slices were incubated in the media of increasing potassium concentration, heavy microsomal specific Na-K-ATPase activity increased, but did not approach control value until medium potassium reached 40 mM. Recovery of Na-K-ATPase activity paralleled recovery of tissue potassium, suggesting the two may be related. 4. 4. Incubation of chicken slices in potassium-free medium had no residual effect on whole homogenate specific Na-K-ATPase activity, although all values were low. 5. 5. Incubation of chicken and snake kidney slices in low sodium medium had no residual effect on Na-K-ATPase activity.

Original languageEnglish (US)
Pages (from-to)79-88
Number of pages10
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume41
Issue number1
DOIs
StatePublished - Jan 15 1972

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Snakes
Adenosine Triphosphatases
Chickens
Potassium
Sodium
Kidney
Sodium-Potassium-Exchanging ATPase
sodium-translocating ATPase
Recovery
Microsomes
Tissue

Keywords

  • chicken kidney slices
  • Gallus domesticus
  • incubation media and Na-K-ATPase
  • low potassium and Na-K-ATPase
  • Na-K-ATPase
  • snake kidney slices
  • Thamnophis sp

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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title = "Effects of incubations in low potassium and low sodium media on Na-K-ATPase activity in snake and chicken kidney slices",
abstract = "1. 1. Effects of incubating snake and chicken kidney slices in media of varying potassium and sodium concentrations on the sodium-potassium-activated adenosine triphosphatase (Na-K-ATPase) activity of these slices were evaluated. 2. 2. Prolonged (2-hr) incubation of chicken kidney slices in potassium-free medium produced significant residual depression in Na-K-ATPase activity in heavy microsomes. Similar incubation of snake kidney slices in potassium-free medium had no residual effect on Na-K-ATPase activity. 3. 3. When potassium-depleted chicken kidney slices were incubated in the media of increasing potassium concentration, heavy microsomal specific Na-K-ATPase activity increased, but did not approach control value until medium potassium reached 40 mM. Recovery of Na-K-ATPase activity paralleled recovery of tissue potassium, suggesting the two may be related. 4. 4. Incubation of chicken slices in potassium-free medium had no residual effect on whole homogenate specific Na-K-ATPase activity, although all values were low. 5. 5. Incubation of chicken and snake kidney slices in low sodium medium had no residual effect on Na-K-ATPase activity.",
keywords = "chicken kidney slices, Gallus domesticus, incubation media and Na-K-ATPase, low potassium and Na-K-ATPase, Na-K-ATPase, snake kidney slices, Thamnophis sp",
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year = "1972",
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N2 - 1. 1. Effects of incubating snake and chicken kidney slices in media of varying potassium and sodium concentrations on the sodium-potassium-activated adenosine triphosphatase (Na-K-ATPase) activity of these slices were evaluated. 2. 2. Prolonged (2-hr) incubation of chicken kidney slices in potassium-free medium produced significant residual depression in Na-K-ATPase activity in heavy microsomes. Similar incubation of snake kidney slices in potassium-free medium had no residual effect on Na-K-ATPase activity. 3. 3. When potassium-depleted chicken kidney slices were incubated in the media of increasing potassium concentration, heavy microsomal specific Na-K-ATPase activity increased, but did not approach control value until medium potassium reached 40 mM. Recovery of Na-K-ATPase activity paralleled recovery of tissue potassium, suggesting the two may be related. 4. 4. Incubation of chicken slices in potassium-free medium had no residual effect on whole homogenate specific Na-K-ATPase activity, although all values were low. 5. 5. Incubation of chicken and snake kidney slices in low sodium medium had no residual effect on Na-K-ATPase activity.

AB - 1. 1. Effects of incubating snake and chicken kidney slices in media of varying potassium and sodium concentrations on the sodium-potassium-activated adenosine triphosphatase (Na-K-ATPase) activity of these slices were evaluated. 2. 2. Prolonged (2-hr) incubation of chicken kidney slices in potassium-free medium produced significant residual depression in Na-K-ATPase activity in heavy microsomes. Similar incubation of snake kidney slices in potassium-free medium had no residual effect on Na-K-ATPase activity. 3. 3. When potassium-depleted chicken kidney slices were incubated in the media of increasing potassium concentration, heavy microsomal specific Na-K-ATPase activity increased, but did not approach control value until medium potassium reached 40 mM. Recovery of Na-K-ATPase activity paralleled recovery of tissue potassium, suggesting the two may be related. 4. 4. Incubation of chicken slices in potassium-free medium had no residual effect on whole homogenate specific Na-K-ATPase activity, although all values were low. 5. 5. Incubation of chicken and snake kidney slices in low sodium medium had no residual effect on Na-K-ATPase activity.

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