Abstract
1. 1. Effects of incubating snake and chicken kidney slices in media of varying potassium and sodium concentrations on the sodium-potassium-activated adenosine triphosphatase (Na-K-ATPase) activity of these slices were evaluated. 2. 2. Prolonged (2-hr) incubation of chicken kidney slices in potassium-free medium produced significant residual depression in Na-K-ATPase activity in heavy microsomes. Similar incubation of snake kidney slices in potassium-free medium had no residual effect on Na-K-ATPase activity. 3. 3. When potassium-depleted chicken kidney slices were incubated in the media of increasing potassium concentration, heavy microsomal specific Na-K-ATPase activity increased, but did not approach control value until medium potassium reached 40 mM. Recovery of Na-K-ATPase activity paralleled recovery of tissue potassium, suggesting the two may be related. 4. 4. Incubation of chicken slices in potassium-free medium had no residual effect on whole homogenate specific Na-K-ATPase activity, although all values were low. 5. 5. Incubation of chicken and snake kidney slices in low sodium medium had no residual effect on Na-K-ATPase activity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 79-88 |
| Number of pages | 10 |
| Journal | Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology |
| Volume | 41 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 15 1972 |
Fingerprint
Keywords
- chicken kidney slices
- Gallus domesticus
- incubation media and Na-K-ATPase
- low potassium and Na-K-ATPase
- Na-K-ATPase
- snake kidney slices
- Thamnophis sp
ASJC Scopus subject areas
- Medicine(all)
Cite this
Effects of incubations in low potassium and low sodium media on Na-K-ATPase activity in snake and chicken kidney slices. / Dantzler, William H.
In: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, Vol. 41, No. 1, 15.01.1972, p. 79-88.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of incubations in low potassium and low sodium media on Na-K-ATPase activity in snake and chicken kidney slices
AU - Dantzler, William H
PY - 1972/1/15
Y1 - 1972/1/15
N2 - 1. 1. Effects of incubating snake and chicken kidney slices in media of varying potassium and sodium concentrations on the sodium-potassium-activated adenosine triphosphatase (Na-K-ATPase) activity of these slices were evaluated. 2. 2. Prolonged (2-hr) incubation of chicken kidney slices in potassium-free medium produced significant residual depression in Na-K-ATPase activity in heavy microsomes. Similar incubation of snake kidney slices in potassium-free medium had no residual effect on Na-K-ATPase activity. 3. 3. When potassium-depleted chicken kidney slices were incubated in the media of increasing potassium concentration, heavy microsomal specific Na-K-ATPase activity increased, but did not approach control value until medium potassium reached 40 mM. Recovery of Na-K-ATPase activity paralleled recovery of tissue potassium, suggesting the two may be related. 4. 4. Incubation of chicken slices in potassium-free medium had no residual effect on whole homogenate specific Na-K-ATPase activity, although all values were low. 5. 5. Incubation of chicken and snake kidney slices in low sodium medium had no residual effect on Na-K-ATPase activity.
AB - 1. 1. Effects of incubating snake and chicken kidney slices in media of varying potassium and sodium concentrations on the sodium-potassium-activated adenosine triphosphatase (Na-K-ATPase) activity of these slices were evaluated. 2. 2. Prolonged (2-hr) incubation of chicken kidney slices in potassium-free medium produced significant residual depression in Na-K-ATPase activity in heavy microsomes. Similar incubation of snake kidney slices in potassium-free medium had no residual effect on Na-K-ATPase activity. 3. 3. When potassium-depleted chicken kidney slices were incubated in the media of increasing potassium concentration, heavy microsomal specific Na-K-ATPase activity increased, but did not approach control value until medium potassium reached 40 mM. Recovery of Na-K-ATPase activity paralleled recovery of tissue potassium, suggesting the two may be related. 4. 4. Incubation of chicken slices in potassium-free medium had no residual effect on whole homogenate specific Na-K-ATPase activity, although all values were low. 5. 5. Incubation of chicken and snake kidney slices in low sodium medium had no residual effect on Na-K-ATPase activity.
KW - chicken kidney slices
KW - Gallus domesticus
KW - incubation media and Na-K-ATPase
KW - low potassium and Na-K-ATPase
KW - Na-K-ATPase
KW - snake kidney slices
KW - Thamnophis sp
UR - http://www.scopus.com/inward/record.url?scp=0015506613&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0015506613&partnerID=8YFLogxK
U2 - 10.1016/0305-0491(72)90009-0
DO - 10.1016/0305-0491(72)90009-0
M3 - Article
C2 - 4259225
AN - SCOPUS:0015506613
VL - 41
SP - 79
EP - 88
JO - Comparative biochemistry and physiology. B, Comparative biochemistry
JF - Comparative biochemistry and physiology. B, Comparative biochemistry
SN - 1096-4959
IS - 1
ER -