TY - JOUR
T1 - Effects of incubations in low potassium and low sodium media on Na-K-ATPase activity in snake and chicken kidney slices
AU - Dantzler, William H.
N1 - Funding Information:
Acknowledgements--The authort hanksM rs. MargueritWe itteand Mrs. SherrilB entleyfo r their valuablete chnicaal ssistance. This investigatiown as supportedb y National ScienceFoundationResearchGrant GB-11788.
PY - 1972/1/15
Y1 - 1972/1/15
N2 - 1. 1. Effects of incubating snake and chicken kidney slices in media of varying potassium and sodium concentrations on the sodium-potassium-activated adenosine triphosphatase (Na-K-ATPase) activity of these slices were evaluated. 2. 2. Prolonged (2-hr) incubation of chicken kidney slices in potassium-free medium produced significant residual depression in Na-K-ATPase activity in heavy microsomes. Similar incubation of snake kidney slices in potassium-free medium had no residual effect on Na-K-ATPase activity. 3. 3. When potassium-depleted chicken kidney slices were incubated in the media of increasing potassium concentration, heavy microsomal specific Na-K-ATPase activity increased, but did not approach control value until medium potassium reached 40 mM. Recovery of Na-K-ATPase activity paralleled recovery of tissue potassium, suggesting the two may be related. 4. 4. Incubation of chicken slices in potassium-free medium had no residual effect on whole homogenate specific Na-K-ATPase activity, although all values were low. 5. 5. Incubation of chicken and snake kidney slices in low sodium medium had no residual effect on Na-K-ATPase activity.
AB - 1. 1. Effects of incubating snake and chicken kidney slices in media of varying potassium and sodium concentrations on the sodium-potassium-activated adenosine triphosphatase (Na-K-ATPase) activity of these slices were evaluated. 2. 2. Prolonged (2-hr) incubation of chicken kidney slices in potassium-free medium produced significant residual depression in Na-K-ATPase activity in heavy microsomes. Similar incubation of snake kidney slices in potassium-free medium had no residual effect on Na-K-ATPase activity. 3. 3. When potassium-depleted chicken kidney slices were incubated in the media of increasing potassium concentration, heavy microsomal specific Na-K-ATPase activity increased, but did not approach control value until medium potassium reached 40 mM. Recovery of Na-K-ATPase activity paralleled recovery of tissue potassium, suggesting the two may be related. 4. 4. Incubation of chicken slices in potassium-free medium had no residual effect on whole homogenate specific Na-K-ATPase activity, although all values were low. 5. 5. Incubation of chicken and snake kidney slices in low sodium medium had no residual effect on Na-K-ATPase activity.
KW - Gallus domesticus
KW - Na-K-ATPase
KW - Thamnophis sp
KW - chicken kidney slices
KW - incubation media and Na-K-ATPase
KW - low potassium and Na-K-ATPase
KW - snake kidney slices
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U2 - 10.1016/0305-0491(72)90009-0
DO - 10.1016/0305-0491(72)90009-0
M3 - Article
C2 - 4259225
AN - SCOPUS:0015506613
VL - 41
SP - 79
EP - 88
JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
SN - 1096-4959
IS - 1
ER -