Effects of lactated ringer's solutions on human leukocytes

Elena Koustova, Kathleen Stanton, Vadim Gushchin, Hasan B. Alam, Svetlana Stegalkina, Peter M Rhee

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Background: The standard lactated Ringer's (LR) solution contains racemic lactate, an equal mixture of D(-)- and L(+)-isomers. The aim of this study was to investigate whether racemic LR solution (containing both isomers, DL-LR) differs from LR containing L-isomer only (L-LR). Methods: Blood from 20 volunteers was incubated for 30 minutes with lactated Ringer's solutions containing the DL- or L-form of lactate, Hank's balanced salt solution, normal saline, and ketone Ringer's (lactate replaced with ketone bodies). Neutrophil "oxidative burst" was measured using flow cytometry. Gene expression of 23 genes associated with leukocyte function was determined with cDNA array technique. The arraying procedure was repeated four times to obtain four sets of data. Results: Compared with the L-LR and ketone Ringer's, DL-LR causes an increased production of reactive oxygen species by neutrophils and affects expression of leukocyte genes known to be involved in inflammation, cell migration, and apoptosis. Conclusion: Lactated Ringer's solution in commonly used formulation (racemic mixture, DL-LR) influences neutrophil function and leukocyte gene expression.

Original languageEnglish (US)
Pages (from-to)872-878
Number of pages7
JournalJournal of Trauma
Volume52
Issue number5
StatePublished - 2002
Externally publishedYes

Fingerprint

Leukocytes
Neutrophils
Ketones
Gene Expression
Lactic Acid
Ringer's lactate
Ringer's solution
L Forms
Ketone Bodies
Respiratory Burst
Oligonucleotide Array Sequence Analysis
Sodium Chloride
Cell Movement
Volunteers
Reactive Oxygen Species
Flow Cytometry
Salts
Apoptosis
Inflammation
Genes

Keywords

  • CDNA array
  • DL-lactate
  • Hemorrhagic shock
  • Ketone bodies
  • L-lactate
  • Lactated Ringer's
  • Leukocyte
  • Resuscitation
  • Resuscitation fluids
  • Transcriptional profiling

ASJC Scopus subject areas

  • Surgery

Cite this

Koustova, E., Stanton, K., Gushchin, V., Alam, H. B., Stegalkina, S., & Rhee, P. M. (2002). Effects of lactated ringer's solutions on human leukocytes. Journal of Trauma, 52(5), 872-878.

Effects of lactated ringer's solutions on human leukocytes. / Koustova, Elena; Stanton, Kathleen; Gushchin, Vadim; Alam, Hasan B.; Stegalkina, Svetlana; Rhee, Peter M.

In: Journal of Trauma, Vol. 52, No. 5, 2002, p. 872-878.

Research output: Contribution to journalArticle

Koustova, E, Stanton, K, Gushchin, V, Alam, HB, Stegalkina, S & Rhee, PM 2002, 'Effects of lactated ringer's solutions on human leukocytes', Journal of Trauma, vol. 52, no. 5, pp. 872-878.
Koustova E, Stanton K, Gushchin V, Alam HB, Stegalkina S, Rhee PM. Effects of lactated ringer's solutions on human leukocytes. Journal of Trauma. 2002;52(5):872-878.
Koustova, Elena ; Stanton, Kathleen ; Gushchin, Vadim ; Alam, Hasan B. ; Stegalkina, Svetlana ; Rhee, Peter M. / Effects of lactated ringer's solutions on human leukocytes. In: Journal of Trauma. 2002 ; Vol. 52, No. 5. pp. 872-878.
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