Effects of nicotine and cotinine on bovine theca intema and granulosa cells

Sara R. Sanders, S Peder Cuneo, Adele M. Turzillo

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

The purpose of this study was to determine if nicotine or cotinine inhibits steroidogenesis in the ovarian follicle. Theca interna and granulosa cells were isolated from bovine follicles, cultured with nicotine or cotinine for 24 h, and culture media were assayed for androstenedione or estradiol. Treatment of theca interna with 6, 60, and 600 μM nicotine decreased (P ≤ 0.002) production of androstenedione to 55, 53, and 24% of control levels, respectively. Levels of androstenedione in theca interna treated with cotinine were not different from control values. In granulosa cells, nicotine inhibited production of estradiol at the highest dose tested. Treatment with 600 μM nicotine decreased (P ≤ 0.001) estradiol concentration to 12% of control values, attributable to a general cytotoxic effect. Cotinine had no effect on estradiol production by granulosa cells. These results provide novel evidence for inhibitory effects of nicotine on androgen production by theca interna.

Original languageEnglish (US)
Pages (from-to)795-800
Number of pages6
JournalReproductive Toxicology
Volume16
Issue number6
DOIs
Publication statusPublished - Nov 2002

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Keywords

  • Androstenedione
  • Cotinine
  • Estradiol
  • Granulosa cells
  • Nicotine
  • Ovary
  • Steroidogenesis
  • Theca cells

ASJC Scopus subject areas

  • Toxicology

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