Effects of ovariectomy on the neuroendocrine axes regulating reproduction and energy balance in young cynomolgus macaques

T. Sandoval-Guzmán, S. T. Stalcup, S. J. Krajewski, M. L. Voytko, Naomi E Rance

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Degeneration of the ovary in middle-aged women results in castrate levels of ovarian steroids and increased gonadotropin secretion from the anterior pituitary gland. Ageing in women is also accompanied by significant changes in energy homeostasis. We have observed alterations in hypothalamic morphology and gene expression in older women, including hypertrophy and increased gene expression of neurokinin B (NKB) neurones, elevated levels of gonadotropin releasing-hormone (GnRH) mRNA and decreased numbers of neurones expressing pro-opiomelanocortin (POMC) mRNA. To determine if loss of ovarian steroids could produce comparable changes in gene expression in young primates, we measured the effects of ovariectomy on NKB, GnRH and POMC gene expression in young cynomolgus monkeys. We also measured serum leptin and body weight to examine the consequences of ovariectomy on energy balance. NKB neurones in the infundibular nucleus of ovariectomized monkeys were larger, more numerous and displayed increased levels of NKB mRNA compared to those of intact controls. Moreover, ovariectomy increased the number of neurones expressing GnRH gene transcripts and elevated serum luteinizing hormone. By contrast, several parameters related to energy balance, including POMC gene expression, serum leptin and body weights, were unchanged by ovariectomy. Thus, the rise in NKB and GnRH gene expression in older women was simulated by ovariectomy in monkeys, but the changes in POMC gene expression and energy balance were not. This study provides strong support for the hypothesis that ovarian failure contributes to the increased NKB and GnRH gene expression observed in postmenopausal women.

Original languageEnglish (US)
Pages (from-to)146-153
Number of pages8
JournalJournal of Neuroendocrinology
Volume16
Issue number2
DOIs
StatePublished - Feb 2004

Fingerprint

Neurokinin B
Macaca
Ovariectomy
Reproduction
Gene Expression
Pro-Opiomelanocortin
Gonadotropin-Releasing Hormone
Neurons
Leptin
Messenger RNA
Haplorhini
Serum
Steroids
Body Weight
Arcuate Nucleus of Hypothalamus
Anterior Pituitary Gland
Macaca fascicularis
Luteinizing Hormone
Gonadotropins
Hypertrophy

Keywords

  • GnRH
  • Hypothalamus
  • Leptin
  • LH
  • Menopause
  • Neurokinin
  • Oestrogen
  • POMC

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)

Cite this

Effects of ovariectomy on the neuroendocrine axes regulating reproduction and energy balance in young cynomolgus macaques. / Sandoval-Guzmán, T.; Stalcup, S. T.; Krajewski, S. J.; Voytko, M. L.; Rance, Naomi E.

In: Journal of Neuroendocrinology, Vol. 16, No. 2, 02.2004, p. 146-153.

Research output: Contribution to journalArticle

Sandoval-Guzmán, T. ; Stalcup, S. T. ; Krajewski, S. J. ; Voytko, M. L. ; Rance, Naomi E. / Effects of ovariectomy on the neuroendocrine axes regulating reproduction and energy balance in young cynomolgus macaques. In: Journal of Neuroendocrinology. 2004 ; Vol. 16, No. 2. pp. 146-153.
@article{18fecb7972d6418886a1fdff8f25735b,
title = "Effects of ovariectomy on the neuroendocrine axes regulating reproduction and energy balance in young cynomolgus macaques",
abstract = "Degeneration of the ovary in middle-aged women results in castrate levels of ovarian steroids and increased gonadotropin secretion from the anterior pituitary gland. Ageing in women is also accompanied by significant changes in energy homeostasis. We have observed alterations in hypothalamic morphology and gene expression in older women, including hypertrophy and increased gene expression of neurokinin B (NKB) neurones, elevated levels of gonadotropin releasing-hormone (GnRH) mRNA and decreased numbers of neurones expressing pro-opiomelanocortin (POMC) mRNA. To determine if loss of ovarian steroids could produce comparable changes in gene expression in young primates, we measured the effects of ovariectomy on NKB, GnRH and POMC gene expression in young cynomolgus monkeys. We also measured serum leptin and body weight to examine the consequences of ovariectomy on energy balance. NKB neurones in the infundibular nucleus of ovariectomized monkeys were larger, more numerous and displayed increased levels of NKB mRNA compared to those of intact controls. Moreover, ovariectomy increased the number of neurones expressing GnRH gene transcripts and elevated serum luteinizing hormone. By contrast, several parameters related to energy balance, including POMC gene expression, serum leptin and body weights, were unchanged by ovariectomy. Thus, the rise in NKB and GnRH gene expression in older women was simulated by ovariectomy in monkeys, but the changes in POMC gene expression and energy balance were not. This study provides strong support for the hypothesis that ovarian failure contributes to the increased NKB and GnRH gene expression observed in postmenopausal women.",
keywords = "GnRH, Hypothalamus, Leptin, LH, Menopause, Neurokinin, Oestrogen, POMC",
author = "T. Sandoval-Guzm{\'a}n and Stalcup, {S. T.} and Krajewski, {S. J.} and Voytko, {M. L.} and Rance, {Naomi E}",
year = "2004",
month = "2",
doi = "10.1111/j.0953-8194.2004.01143.x",
language = "English (US)",
volume = "16",
pages = "146--153",
journal = "Journal of Neuroendocrinology",
issn = "0953-8194",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Effects of ovariectomy on the neuroendocrine axes regulating reproduction and energy balance in young cynomolgus macaques

AU - Sandoval-Guzmán, T.

AU - Stalcup, S. T.

AU - Krajewski, S. J.

AU - Voytko, M. L.

AU - Rance, Naomi E

PY - 2004/2

Y1 - 2004/2

N2 - Degeneration of the ovary in middle-aged women results in castrate levels of ovarian steroids and increased gonadotropin secretion from the anterior pituitary gland. Ageing in women is also accompanied by significant changes in energy homeostasis. We have observed alterations in hypothalamic morphology and gene expression in older women, including hypertrophy and increased gene expression of neurokinin B (NKB) neurones, elevated levels of gonadotropin releasing-hormone (GnRH) mRNA and decreased numbers of neurones expressing pro-opiomelanocortin (POMC) mRNA. To determine if loss of ovarian steroids could produce comparable changes in gene expression in young primates, we measured the effects of ovariectomy on NKB, GnRH and POMC gene expression in young cynomolgus monkeys. We also measured serum leptin and body weight to examine the consequences of ovariectomy on energy balance. NKB neurones in the infundibular nucleus of ovariectomized monkeys were larger, more numerous and displayed increased levels of NKB mRNA compared to those of intact controls. Moreover, ovariectomy increased the number of neurones expressing GnRH gene transcripts and elevated serum luteinizing hormone. By contrast, several parameters related to energy balance, including POMC gene expression, serum leptin and body weights, were unchanged by ovariectomy. Thus, the rise in NKB and GnRH gene expression in older women was simulated by ovariectomy in monkeys, but the changes in POMC gene expression and energy balance were not. This study provides strong support for the hypothesis that ovarian failure contributes to the increased NKB and GnRH gene expression observed in postmenopausal women.

AB - Degeneration of the ovary in middle-aged women results in castrate levels of ovarian steroids and increased gonadotropin secretion from the anterior pituitary gland. Ageing in women is also accompanied by significant changes in energy homeostasis. We have observed alterations in hypothalamic morphology and gene expression in older women, including hypertrophy and increased gene expression of neurokinin B (NKB) neurones, elevated levels of gonadotropin releasing-hormone (GnRH) mRNA and decreased numbers of neurones expressing pro-opiomelanocortin (POMC) mRNA. To determine if loss of ovarian steroids could produce comparable changes in gene expression in young primates, we measured the effects of ovariectomy on NKB, GnRH and POMC gene expression in young cynomolgus monkeys. We also measured serum leptin and body weight to examine the consequences of ovariectomy on energy balance. NKB neurones in the infundibular nucleus of ovariectomized monkeys were larger, more numerous and displayed increased levels of NKB mRNA compared to those of intact controls. Moreover, ovariectomy increased the number of neurones expressing GnRH gene transcripts and elevated serum luteinizing hormone. By contrast, several parameters related to energy balance, including POMC gene expression, serum leptin and body weights, were unchanged by ovariectomy. Thus, the rise in NKB and GnRH gene expression in older women was simulated by ovariectomy in monkeys, but the changes in POMC gene expression and energy balance were not. This study provides strong support for the hypothesis that ovarian failure contributes to the increased NKB and GnRH gene expression observed in postmenopausal women.

KW - GnRH

KW - Hypothalamus

KW - Leptin

KW - LH

KW - Menopause

KW - Neurokinin

KW - Oestrogen

KW - POMC

UR - http://www.scopus.com/inward/record.url?scp=1342290357&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1342290357&partnerID=8YFLogxK

U2 - 10.1111/j.0953-8194.2004.01143.x

DO - 10.1111/j.0953-8194.2004.01143.x

M3 - Article

C2 - 14764001

AN - SCOPUS:1342290357

VL - 16

SP - 146

EP - 153

JO - Journal of Neuroendocrinology

JF - Journal of Neuroendocrinology

SN - 0953-8194

IS - 2

ER -