Effects of oxygen on metabolic and secretory activities of βTC3 cells

Klearchos K Papas, R. C. Long, I. Constantinidis, A. Sambanis

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

We have investigated the rates of glucose consumption, lactate production and insulin secretion by mouse insulinoma βTC3 cells exposed to high glucose and oxygen concentrations in the range of 132 mmHg (normoxia) to 0 mmHg (anoxia). The rates of glucose consumption and lactate production, and the yield of lactate on glucose were 6.4 ± 0.2 nmol/h - 105 cells, 7.7 ± 0.5 nmol/h - 105 cells, and 1.2 ± 0.1 respectively, at oxygen concentrations between 132-25 mmHg. These values increased gradually as the oxygen concentration was reduced below 25 mmHg, reaching a maximum value of 12.8 ± 0.4, 23.8 ± 1.1, 1.9 ± 0.1 respectively, at complete anoxia. Insulin secretion remained constant at 360 ± 24 pmol/h - 108 cells at oxygen concentrations between 132-7 mmHg, but was inhibited at lower oxygen concentrations, dropping to 96 ± 24 pmol/h - 108 cells at 0 mmHg. The rate of insulin secretion in the presence of high glucose under anoxia was significantly higher than the rate of basal secretion (28.2 ± 3.0 pmol/h - 108 cells) at normoxia. The secretory properties of βTC3 cells at low oxygen concentrations may have implications in the development of a diffusion-based bioartificial tissue constructs for the long-term treatment of Insulin Dependent Diabetes Mellitus.

Original languageEnglish (US)
Pages (from-to)163-166
Number of pages4
JournalBBA - General Subjects
Volume1291
Issue number2
DOIs
StatePublished - Oct 24 1996
Externally publishedYes

Fingerprint

Oxygen
Glucose
Insulin
Lactic Acid
Insulinoma
Medical problems
Type 1 Diabetes Mellitus
Tissue
Hypoxia

Keywords

  • βTC3
  • Anoxia
  • Bioartificial pancreas
  • Hypoxia
  • Insulin secretion

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Effects of oxygen on metabolic and secretory activities of βTC3 cells. / Papas, Klearchos K; Long, R. C.; Constantinidis, I.; Sambanis, A.

In: BBA - General Subjects, Vol. 1291, No. 2, 24.10.1996, p. 163-166.

Research output: Contribution to journalArticle

Papas, Klearchos K ; Long, R. C. ; Constantinidis, I. ; Sambanis, A. / Effects of oxygen on metabolic and secretory activities of βTC3 cells. In: BBA - General Subjects. 1996 ; Vol. 1291, No. 2. pp. 163-166.
@article{e332b8c7ccb74e4f8307047c3e6255d7,
title = "Effects of oxygen on metabolic and secretory activities of βTC3 cells",
abstract = "We have investigated the rates of glucose consumption, lactate production and insulin secretion by mouse insulinoma βTC3 cells exposed to high glucose and oxygen concentrations in the range of 132 mmHg (normoxia) to 0 mmHg (anoxia). The rates of glucose consumption and lactate production, and the yield of lactate on glucose were 6.4 ± 0.2 nmol/h - 105 cells, 7.7 ± 0.5 nmol/h - 105 cells, and 1.2 ± 0.1 respectively, at oxygen concentrations between 132-25 mmHg. These values increased gradually as the oxygen concentration was reduced below 25 mmHg, reaching a maximum value of 12.8 ± 0.4, 23.8 ± 1.1, 1.9 ± 0.1 respectively, at complete anoxia. Insulin secretion remained constant at 360 ± 24 pmol/h - 108 cells at oxygen concentrations between 132-7 mmHg, but was inhibited at lower oxygen concentrations, dropping to 96 ± 24 pmol/h - 108 cells at 0 mmHg. The rate of insulin secretion in the presence of high glucose under anoxia was significantly higher than the rate of basal secretion (28.2 ± 3.0 pmol/h - 108 cells) at normoxia. The secretory properties of βTC3 cells at low oxygen concentrations may have implications in the development of a diffusion-based bioartificial tissue constructs for the long-term treatment of Insulin Dependent Diabetes Mellitus.",
keywords = "βTC3, Anoxia, Bioartificial pancreas, Hypoxia, Insulin secretion",
author = "Papas, {Klearchos K} and Long, {R. C.} and I. Constantinidis and A. Sambanis",
year = "1996",
month = "10",
day = "24",
doi = "10.1016/0304-4165(96)00062-1",
language = "English (US)",
volume = "1291",
pages = "163--166",
journal = "Biochimica et Biophysica Acta - General Subjects",
issn = "0304-4165",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Effects of oxygen on metabolic and secretory activities of βTC3 cells

AU - Papas, Klearchos K

AU - Long, R. C.

AU - Constantinidis, I.

AU - Sambanis, A.

PY - 1996/10/24

Y1 - 1996/10/24

N2 - We have investigated the rates of glucose consumption, lactate production and insulin secretion by mouse insulinoma βTC3 cells exposed to high glucose and oxygen concentrations in the range of 132 mmHg (normoxia) to 0 mmHg (anoxia). The rates of glucose consumption and lactate production, and the yield of lactate on glucose were 6.4 ± 0.2 nmol/h - 105 cells, 7.7 ± 0.5 nmol/h - 105 cells, and 1.2 ± 0.1 respectively, at oxygen concentrations between 132-25 mmHg. These values increased gradually as the oxygen concentration was reduced below 25 mmHg, reaching a maximum value of 12.8 ± 0.4, 23.8 ± 1.1, 1.9 ± 0.1 respectively, at complete anoxia. Insulin secretion remained constant at 360 ± 24 pmol/h - 108 cells at oxygen concentrations between 132-7 mmHg, but was inhibited at lower oxygen concentrations, dropping to 96 ± 24 pmol/h - 108 cells at 0 mmHg. The rate of insulin secretion in the presence of high glucose under anoxia was significantly higher than the rate of basal secretion (28.2 ± 3.0 pmol/h - 108 cells) at normoxia. The secretory properties of βTC3 cells at low oxygen concentrations may have implications in the development of a diffusion-based bioartificial tissue constructs for the long-term treatment of Insulin Dependent Diabetes Mellitus.

AB - We have investigated the rates of glucose consumption, lactate production and insulin secretion by mouse insulinoma βTC3 cells exposed to high glucose and oxygen concentrations in the range of 132 mmHg (normoxia) to 0 mmHg (anoxia). The rates of glucose consumption and lactate production, and the yield of lactate on glucose were 6.4 ± 0.2 nmol/h - 105 cells, 7.7 ± 0.5 nmol/h - 105 cells, and 1.2 ± 0.1 respectively, at oxygen concentrations between 132-25 mmHg. These values increased gradually as the oxygen concentration was reduced below 25 mmHg, reaching a maximum value of 12.8 ± 0.4, 23.8 ± 1.1, 1.9 ± 0.1 respectively, at complete anoxia. Insulin secretion remained constant at 360 ± 24 pmol/h - 108 cells at oxygen concentrations between 132-7 mmHg, but was inhibited at lower oxygen concentrations, dropping to 96 ± 24 pmol/h - 108 cells at 0 mmHg. The rate of insulin secretion in the presence of high glucose under anoxia was significantly higher than the rate of basal secretion (28.2 ± 3.0 pmol/h - 108 cells) at normoxia. The secretory properties of βTC3 cells at low oxygen concentrations may have implications in the development of a diffusion-based bioartificial tissue constructs for the long-term treatment of Insulin Dependent Diabetes Mellitus.

KW - βTC3

KW - Anoxia

KW - Bioartificial pancreas

KW - Hypoxia

KW - Insulin secretion

UR - http://www.scopus.com/inward/record.url?scp=0030600502&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030600502&partnerID=8YFLogxK

U2 - 10.1016/0304-4165(96)00062-1

DO - 10.1016/0304-4165(96)00062-1

M3 - Article

C2 - 8898878

AN - SCOPUS:0030600502

VL - 1291

SP - 163

EP - 166

JO - Biochimica et Biophysica Acta - General Subjects

JF - Biochimica et Biophysica Acta - General Subjects

SN - 0304-4165

IS - 2

ER -