Effects of oxygen on metabolic and secretory activities of βTC3 cells

K. K. Papas, R. C. Long, I. Constantinidis, A. Sambanis

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

We have investigated the rates of glucose consumption, lactate production and insulin secretion by mouse insulinoma βTC3 cells exposed to high glucose and oxygen concentrations in the range of 132 mmHg (normoxia) to 0 mmHg (anoxia). The rates of glucose consumption and lactate production, and the yield of lactate on glucose were 6.4 ± 0.2 nmol/h - 105 cells, 7.7 ± 0.5 nmol/h - 105 cells, and 1.2 ± 0.1 respectively, at oxygen concentrations between 132-25 mmHg. These values increased gradually as the oxygen concentration was reduced below 25 mmHg, reaching a maximum value of 12.8 ± 0.4, 23.8 ± 1.1, 1.9 ± 0.1 respectively, at complete anoxia. Insulin secretion remained constant at 360 ± 24 pmol/h - 108 cells at oxygen concentrations between 132-7 mmHg, but was inhibited at lower oxygen concentrations, dropping to 96 ± 24 pmol/h - 108 cells at 0 mmHg. The rate of insulin secretion in the presence of high glucose under anoxia was significantly higher than the rate of basal secretion (28.2 ± 3.0 pmol/h - 108 cells) at normoxia. The secretory properties of βTC3 cells at low oxygen concentrations may have implications in the development of a diffusion-based bioartificial tissue constructs for the long-term treatment of Insulin Dependent Diabetes Mellitus.

Original languageEnglish (US)
Pages (from-to)163-166
Number of pages4
JournalBiochimica et Biophysica Acta - General Subjects
Volume1291
Issue number2
DOIs
StatePublished - Oct 24 1996
Externally publishedYes

Keywords

  • Anoxia
  • Bioartificial pancreas
  • Hypoxia
  • Insulin secretion
  • βTC3

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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