Effects of pertussis toxin on vasodilation and cyclic GMP in bovine mesenteric arteries and demonstration of a 40 kD soluble protein ribosylation substrate for pertussis toxin

M. Ekstam Ljusegren, K. L. Axelsson, J. Ahlner, J. O G Karlsson, R. G G Andersson, B. R. Magnusson, Richard L Friedman

Research output: Contribution to journalArticle

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Abstract

The inhibitory nucleotide-regulatory protein (Gl) has been shown to lose its adenylate cyclase inhibitory effect upon treatment with pertussis toxin. To find out whether a pertussis sensitive mechanism is involved in the regulation of the cGMP-system, bovine mesenteric arteries were incubated in buffer containing pertussis toxin, and the relaxation and intracellular cGMP accumulation induced by different groups of vasodilating agents were studied. The present results show a pertussis toxin induced decrease in relaxation as well as a decrease in the cGMP-elevation induced by the endothelium dependent vasodilators acetylcholine and calcium ionophore A 23187. Arteries treated with artrial natriuretic peptide showed no alterations in relaxation of cGMP content after incubation with pertussis toxin. A 40 kD soluble ribosylation substrate for pertussis toxin was identified in bovine mesenteric artery. These results suggest that a pertussis toxin sensitive mechanism is involved in the vasodilating mechanism of acetylcholine and calcium ionophore A 23187, while no evidence for such a mechanism could be found regarding the vasodilatory action of atrial natriuretic peptide.

Original languageEnglish (US)
Pages (from-to)543-552
Number of pages10
JournalLife Sciences
Volume46
Issue number8
DOIs
StatePublished - 1990

Fingerprint

Mesenteric Arteries
Cyclic GMP
Pertussis Toxin
Vasodilation
Demonstrations
Substrates
Proteins
Calcium Ionophores
Calcimycin
Acetylcholine
Endothelium-Dependent Relaxing Factors
Natriuretic Peptides
Whooping Cough
Atrial Natriuretic Factor
Adenylyl Cyclases
Buffers
Nucleotides
Arteries

ASJC Scopus subject areas

  • Pharmacology

Cite this

Effects of pertussis toxin on vasodilation and cyclic GMP in bovine mesenteric arteries and demonstration of a 40 kD soluble protein ribosylation substrate for pertussis toxin. / Ljusegren, M. Ekstam; Axelsson, K. L.; Ahlner, J.; Karlsson, J. O G; Andersson, R. G G; Magnusson, B. R.; Friedman, Richard L.

In: Life Sciences, Vol. 46, No. 8, 1990, p. 543-552.

Research output: Contribution to journalArticle

Ljusegren, M. Ekstam ; Axelsson, K. L. ; Ahlner, J. ; Karlsson, J. O G ; Andersson, R. G G ; Magnusson, B. R. ; Friedman, Richard L. / Effects of pertussis toxin on vasodilation and cyclic GMP in bovine mesenteric arteries and demonstration of a 40 kD soluble protein ribosylation substrate for pertussis toxin. In: Life Sciences. 1990 ; Vol. 46, No. 8. pp. 543-552.
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abstract = "The inhibitory nucleotide-regulatory protein (Gl) has been shown to lose its adenylate cyclase inhibitory effect upon treatment with pertussis toxin. To find out whether a pertussis sensitive mechanism is involved in the regulation of the cGMP-system, bovine mesenteric arteries were incubated in buffer containing pertussis toxin, and the relaxation and intracellular cGMP accumulation induced by different groups of vasodilating agents were studied. The present results show a pertussis toxin induced decrease in relaxation as well as a decrease in the cGMP-elevation induced by the endothelium dependent vasodilators acetylcholine and calcium ionophore A 23187. Arteries treated with artrial natriuretic peptide showed no alterations in relaxation of cGMP content after incubation with pertussis toxin. A 40 kD soluble ribosylation substrate for pertussis toxin was identified in bovine mesenteric artery. These results suggest that a pertussis toxin sensitive mechanism is involved in the vasodilating mechanism of acetylcholine and calcium ionophore A 23187, while no evidence for such a mechanism could be found regarding the vasodilatory action of atrial natriuretic peptide.",
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AU - Axelsson, K. L.

AU - Ahlner, J.

AU - Karlsson, J. O G

AU - Andersson, R. G G

AU - Magnusson, B. R.

AU - Friedman, Richard L

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AB - The inhibitory nucleotide-regulatory protein (Gl) has been shown to lose its adenylate cyclase inhibitory effect upon treatment with pertussis toxin. To find out whether a pertussis sensitive mechanism is involved in the regulation of the cGMP-system, bovine mesenteric arteries were incubated in buffer containing pertussis toxin, and the relaxation and intracellular cGMP accumulation induced by different groups of vasodilating agents were studied. The present results show a pertussis toxin induced decrease in relaxation as well as a decrease in the cGMP-elevation induced by the endothelium dependent vasodilators acetylcholine and calcium ionophore A 23187. Arteries treated with artrial natriuretic peptide showed no alterations in relaxation of cGMP content after incubation with pertussis toxin. A 40 kD soluble ribosylation substrate for pertussis toxin was identified in bovine mesenteric artery. These results suggest that a pertussis toxin sensitive mechanism is involved in the vasodilating mechanism of acetylcholine and calcium ionophore A 23187, while no evidence for such a mechanism could be found regarding the vasodilatory action of atrial natriuretic peptide.

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