TY - JOUR
T1 - Effects of pH on murine insulinoma βTC3 cells
AU - Simpson, Nicholas E.
AU - Bennett, Lindsey K.
AU - Papas, Klearchos K.
AU - Sambanis, Athanassios
AU - Constantinidis, Ioannis
N1 - Funding Information:
The authors are grateful for the technical assistance of Cherie Stabler, Frank Fusaro, and Dr. Robert Long. Financial support was in part provided by grants from the NIH (DK-47858 and F32 DK-09941), the NSF (BES-9410703 and EEC-9731643), and the Georgia Research Alliance.
PY - 2000/7/14
Y1 - 2000/7/14
N2 - Confluent monolayer cultures of βTC3 cells were exposed for 4 h to acidic, neutral, or alkaline pH media. Studies determined the impact of pH on viability, insulin secretion rate, glucose consumption rate, lactate production rate, and ATP content. Cell viability was not affected by exposure to media of different pH (> 95% for all groups). Insulin release from cells exposed to acidic media (pH of 6.4) was ~ 75% higher than that from cells exposed to either neutral (pH of 7.1) or alkaline (pH of 7.8) conditions. Conversely, ATP content was significantly reduced in cultures exposed to acidic conditions, although there was no statistical difference between neutral and alkaline conditions. Glucose consumption and lactate production rates increased linearly with increasing pH. (C) 2000 Academic Press.
AB - Confluent monolayer cultures of βTC3 cells were exposed for 4 h to acidic, neutral, or alkaline pH media. Studies determined the impact of pH on viability, insulin secretion rate, glucose consumption rate, lactate production rate, and ATP content. Cell viability was not affected by exposure to media of different pH (> 95% for all groups). Insulin release from cells exposed to acidic media (pH of 6.4) was ~ 75% higher than that from cells exposed to either neutral (pH of 7.1) or alkaline (pH of 7.8) conditions. Conversely, ATP content was significantly reduced in cultures exposed to acidic conditions, although there was no statistical difference between neutral and alkaline conditions. Glucose consumption and lactate production rates increased linearly with increasing pH. (C) 2000 Academic Press.
KW - Bicarbonate
KW - Insulin secretion
KW - Monolayer
KW - Transformed insulinoma
KW - pH
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U2 - 10.1006/bbrc.2000.3022
DO - 10.1006/bbrc.2000.3022
M3 - Article
C2 - 10891351
AN - SCOPUS:0034647931
VL - 273
SP - 937
EP - 941
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -