Effects of pH on murine insulinoma βTC3 cells

Nicholas E. Simpson; Lindsey K. Bennett; Klearchos K. Papas; Athanassios Sambanis; Ioannis Constantinidis

doi:10.1006/bbrc.2000.3022

Effects of pH on murine insulinoma βTC3 cells

Nicholas E. Simpson, Lindsey K. Bennett, Klearchos K. Papas, Athanassios Sambanis, Ioannis Constantinidis

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Confluent monolayer cultures of βTC3 cells were exposed for 4 h to acidic, neutral, or alkaline pH media. Studies determined the impact of pH on viability, insulin secretion rate, glucose consumption rate, lactate production rate, and ATP content. Cell viability was not affected by exposure to media of different pH (> 95% for all groups). Insulin release from cells exposed to acidic media (pH of 6.4) was ~ 75% higher than that from cells exposed to either neutral (pH of 7.1) or alkaline (pH of 7.8) conditions. Conversely, ATP content was significantly reduced in cultures exposed to acidic conditions, although there was no statistical difference between neutral and alkaline conditions. Glucose consumption and lactate production rates increased linearly with increasing pH. (C) 2000 Academic Press.

Original language	English (US)
Pages (from-to)	937-941
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	273
Issue number	3
DOIs	https://doi.org/10.1006/bbrc.2000.3022
State	Published - Jul 14 2000
Externally published	Yes

Keywords

Bicarbonate
Insulin secretion
Monolayer
Transformed insulinoma
pH

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Effects of pH on murine insulinoma βTC3 cells",

abstract = "Confluent monolayer cultures of βTC3 cells were exposed for 4 h to acidic, neutral, or alkaline pH media. Studies determined the impact of pH on viability, insulin secretion rate, glucose consumption rate, lactate production rate, and ATP content. Cell viability was not affected by exposure to media of different pH (> 95% for all groups). Insulin release from cells exposed to acidic media (pH of 6.4) was ~ 75% higher than that from cells exposed to either neutral (pH of 7.1) or alkaline (pH of 7.8) conditions. Conversely, ATP content was significantly reduced in cultures exposed to acidic conditions, although there was no statistical difference between neutral and alkaline conditions. Glucose consumption and lactate production rates increased linearly with increasing pH. (C) 2000 Academic Press.",

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AU - Bennett, Lindsey K.

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AU - Sambanis, Athanassios

AU - Constantinidis, Ioannis

PY - 2000/7/14

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AB - Confluent monolayer cultures of βTC3 cells were exposed for 4 h to acidic, neutral, or alkaline pH media. Studies determined the impact of pH on viability, insulin secretion rate, glucose consumption rate, lactate production rate, and ATP content. Cell viability was not affected by exposure to media of different pH (> 95% for all groups). Insulin release from cells exposed to acidic media (pH of 6.4) was ~ 75% higher than that from cells exposed to either neutral (pH of 7.1) or alkaline (pH of 7.8) conditions. Conversely, ATP content was significantly reduced in cultures exposed to acidic conditions, although there was no statistical difference between neutral and alkaline conditions. Glucose consumption and lactate production rates increased linearly with increasing pH. (C) 2000 Academic Press.

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KW - Insulin secretion

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KW - Transformed insulinoma

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Effects of pH on murine insulinoma βTC3 cells

Abstract

Keywords

ASJC Scopus subject areas

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