Effects of pH on murine insulinoma βTC3 cells

Nicholas E. Simpson, Lindsey K. Bennett, Klearchos K Papas, Athanassios Sambanis, Ioannis Constantinidis

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Confluent monolayer cultures of βTC3 cells were exposed for 4 h to acidic, neutral, or alkaline pH media. Studies determined the impact of pH on viability, insulin secretion rate, glucose consumption rate, lactate production rate, and ATP content. Cell viability was not affected by exposure to media of different pH (> 95% for all groups). Insulin release from cells exposed to acidic media (pH of 6.4) was ~ 75% higher than that from cells exposed to either neutral (pH of 7.1) or alkaline (pH of 7.8) conditions. Conversely, ATP content was significantly reduced in cultures exposed to acidic conditions, although there was no statistical difference between neutral and alkaline conditions. Glucose consumption and lactate production rates increased linearly with increasing pH. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)937-941
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume273
Issue number3
DOIs
StatePublished - Jul 14 2000
Externally publishedYes

Fingerprint

Insulinoma
Lactic Acid
Adenosine Triphosphate
Insulin
Glucose
Monolayers
Cells
Cell Survival
Cell Culture Techniques

Keywords

  • Bicarbonate
  • Insulin secretion
  • Monolayer
  • pH
  • Transformed insulinoma

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Effects of pH on murine insulinoma βTC3 cells. / Simpson, Nicholas E.; Bennett, Lindsey K.; Papas, Klearchos K; Sambanis, Athanassios; Constantinidis, Ioannis.

In: Biochemical and Biophysical Research Communications, Vol. 273, No. 3, 14.07.2000, p. 937-941.

Research output: Contribution to journalArticle

Simpson, NE, Bennett, LK, Papas, KK, Sambanis, A & Constantinidis, I 2000, 'Effects of pH on murine insulinoma βTC3 cells', Biochemical and Biophysical Research Communications, vol. 273, no. 3, pp. 937-941. https://doi.org/10.1006/bbrc.2000.3022
Simpson, Nicholas E. ; Bennett, Lindsey K. ; Papas, Klearchos K ; Sambanis, Athanassios ; Constantinidis, Ioannis. / Effects of pH on murine insulinoma βTC3 cells. In: Biochemical and Biophysical Research Communications. 2000 ; Vol. 273, No. 3. pp. 937-941.
@article{6391a72457054112a94a91c2e305b9d1,
title = "Effects of pH on murine insulinoma βTC3 cells",
abstract = "Confluent monolayer cultures of βTC3 cells were exposed for 4 h to acidic, neutral, or alkaline pH media. Studies determined the impact of pH on viability, insulin secretion rate, glucose consumption rate, lactate production rate, and ATP content. Cell viability was not affected by exposure to media of different pH (> 95{\%} for all groups). Insulin release from cells exposed to acidic media (pH of 6.4) was ~ 75{\%} higher than that from cells exposed to either neutral (pH of 7.1) or alkaline (pH of 7.8) conditions. Conversely, ATP content was significantly reduced in cultures exposed to acidic conditions, although there was no statistical difference between neutral and alkaline conditions. Glucose consumption and lactate production rates increased linearly with increasing pH. (C) 2000 Academic Press.",
keywords = "Bicarbonate, Insulin secretion, Monolayer, pH, Transformed insulinoma",
author = "Simpson, {Nicholas E.} and Bennett, {Lindsey K.} and Papas, {Klearchos K} and Athanassios Sambanis and Ioannis Constantinidis",
year = "2000",
month = "7",
day = "14",
doi = "10.1006/bbrc.2000.3022",
language = "English (US)",
volume = "273",
pages = "937--941",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Effects of pH on murine insulinoma βTC3 cells

AU - Simpson, Nicholas E.

AU - Bennett, Lindsey K.

AU - Papas, Klearchos K

AU - Sambanis, Athanassios

AU - Constantinidis, Ioannis

PY - 2000/7/14

Y1 - 2000/7/14

N2 - Confluent monolayer cultures of βTC3 cells were exposed for 4 h to acidic, neutral, or alkaline pH media. Studies determined the impact of pH on viability, insulin secretion rate, glucose consumption rate, lactate production rate, and ATP content. Cell viability was not affected by exposure to media of different pH (> 95% for all groups). Insulin release from cells exposed to acidic media (pH of 6.4) was ~ 75% higher than that from cells exposed to either neutral (pH of 7.1) or alkaline (pH of 7.8) conditions. Conversely, ATP content was significantly reduced in cultures exposed to acidic conditions, although there was no statistical difference between neutral and alkaline conditions. Glucose consumption and lactate production rates increased linearly with increasing pH. (C) 2000 Academic Press.

AB - Confluent monolayer cultures of βTC3 cells were exposed for 4 h to acidic, neutral, or alkaline pH media. Studies determined the impact of pH on viability, insulin secretion rate, glucose consumption rate, lactate production rate, and ATP content. Cell viability was not affected by exposure to media of different pH (> 95% for all groups). Insulin release from cells exposed to acidic media (pH of 6.4) was ~ 75% higher than that from cells exposed to either neutral (pH of 7.1) or alkaline (pH of 7.8) conditions. Conversely, ATP content was significantly reduced in cultures exposed to acidic conditions, although there was no statistical difference between neutral and alkaline conditions. Glucose consumption and lactate production rates increased linearly with increasing pH. (C) 2000 Academic Press.

KW - Bicarbonate

KW - Insulin secretion

KW - Monolayer

KW - pH

KW - Transformed insulinoma

UR - http://www.scopus.com/inward/record.url?scp=0034647931&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034647931&partnerID=8YFLogxK

U2 - 10.1006/bbrc.2000.3022

DO - 10.1006/bbrc.2000.3022

M3 - Article

C2 - 10891351

AN - SCOPUS:0034647931

VL - 273

SP - 937

EP - 941

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -