Effects of protein malnutrition on liver cytochrome P450s

Ping Cheung Lee, Mark F. Struve, Jorge A. Bezerra, Burris R Duncan

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Microsomal cytochrome P450s (CYPs) are the key enzymes that control the rate of drag metabolism. In turn, hepatic drag biotransformation is influenced by dietary macronutrients. We examined the effects of protein malnutrition on hepatic CYP1A and 3A in rats. Weanling male rats were fed protein-restricted diets with either 0.5% or 1.0% lactalbumin for 13 weeks. Control rats were fed similar diets but with 18% lactalbumin. Severe protein malnutrition resulted in rats fed the 0.5% and 1.0% lactalbumin diets as evidenced by stunting of growth, lowering of hematocrit and plasma albumin concentrations. Protein malnourished rats had a reduction in their hepatic microsomal concentrations only when expressed as per gm tissue but not as per protein concentration. Western blot analyses showed that protein malnutrition had no effect on hepatic CYP1A1 and only effected CYP1A2 at the most severe conditions (ie. in rats fed the 0.5% lactalbumin diet). Protein malnutrition however, led to >50% reduction in hepatic microsomal CYP3A proteins. Liver microsomal steroid metabolizing enzyme activities were evaluated by quantitation of specific metabolites formed following incubation with 4- C14 progesterone and TLC separation. Protein malnutrition led to reductions in both 6β- and 16 α-progesterone hydroxylase, but did not affect either 17α-hydroxylase or 5α-reductase activity. Protein malnutrition, thus, modulates the various CYPs differently. Such changes in hepatic CYP concentrations might he the cause of altered drag metabolism accompanying protein malnutrition.

Original languageEnglish (US)
Pages (from-to)1577-1587
Number of pages11
JournalNutrition Research
Volume17
Issue number10
DOIs
StatePublished - Oct 1997

Fingerprint

Cytochromes
Malnutrition
Liver
Lactalbumin
Proteins
Diet
Growth Disorders
Cytochrome P-450 CYP3A
Cytochrome P-450 CYP1A2
Protein-Restricted Diet
Cytochrome P-450 CYP1A1
Enzymes
Biotransformation
Mixed Function Oxygenases
Hematocrit
Serum Albumin
Progesterone
Oxidoreductases
Western Blotting
Steroids

Keywords

  • Biotransformation
  • Cytochrome P450
  • Malnutrition

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Effects of protein malnutrition on liver cytochrome P450s. / Lee, Ping Cheung; Struve, Mark F.; Bezerra, Jorge A.; Duncan, Burris R.

In: Nutrition Research, Vol. 17, No. 10, 10.1997, p. 1577-1587.

Research output: Contribution to journalArticle

Lee, Ping Cheung ; Struve, Mark F. ; Bezerra, Jorge A. ; Duncan, Burris R. / Effects of protein malnutrition on liver cytochrome P450s. In: Nutrition Research. 1997 ; Vol. 17, No. 10. pp. 1577-1587.
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