Effects of retinoic acid (RA) on the growth and phenotypic expression of several human neuroblastoma cell lines

Neil Sidell, Adrienne Altman, Mark R Haussler, Robert C. Seeger

Research output: Contribution to journalArticle

261 Citations (Scopus)

Abstract

It has been shown that retinoic acid (RA) can promote morphologic differentiation and inhibit the growth of a human neuroblastoma cell line, LA-N-1. The present study tests the histological generality of these phenomena by determining the effects of RA on seven other human neuroblastoma cell lines. Results show that RA strongly inhibited anchorage-dependent growth and induced morphologic alterations in six of seven of the cell lines. These alterations included morphologic differentiation as evidenced by formation of neurite extensions in four of the lines, cellular enlargement and vacuolization in one culture, and formation of large, flattened epithelial or fibroblastic-like cells in another culture. Although one cell line was relatively insensitive to the effects of RA in monolayer culture, all seven were strongly inhibited by RA in soft agar assays. Cellular RA-binding proteins were detected in 2/2 lines tested. These findings suggest that, as a histological group, human neuroblastoma cells are extremely sensitive to RA-induced growth inhibition and morphological alterations generally associated with reduced expression of the malignant phenotype of this type of cancer.

Original languageEnglish (US)
Pages (from-to)21-30
Number of pages10
JournalExperimental Cell Research
Volume148
Issue number1
DOIs
StatePublished - Oct 1 1983

Fingerprint

Tretinoin
Neuroblastoma
Cell Line
Growth
Retinoic Acid Receptors
Neurites
Agar
Phenotype
Neoplasms

ASJC Scopus subject areas

  • Cell Biology

Cite this

Effects of retinoic acid (RA) on the growth and phenotypic expression of several human neuroblastoma cell lines. / Sidell, Neil; Altman, Adrienne; Haussler, Mark R; Seeger, Robert C.

In: Experimental Cell Research, Vol. 148, No. 1, 01.10.1983, p. 21-30.

Research output: Contribution to journalArticle

@article{13c3dd7475974e0ea175cedecf3058bf,
title = "Effects of retinoic acid (RA) on the growth and phenotypic expression of several human neuroblastoma cell lines",
abstract = "It has been shown that retinoic acid (RA) can promote morphologic differentiation and inhibit the growth of a human neuroblastoma cell line, LA-N-1. The present study tests the histological generality of these phenomena by determining the effects of RA on seven other human neuroblastoma cell lines. Results show that RA strongly inhibited anchorage-dependent growth and induced morphologic alterations in six of seven of the cell lines. These alterations included morphologic differentiation as evidenced by formation of neurite extensions in four of the lines, cellular enlargement and vacuolization in one culture, and formation of large, flattened epithelial or fibroblastic-like cells in another culture. Although one cell line was relatively insensitive to the effects of RA in monolayer culture, all seven were strongly inhibited by RA in soft agar assays. Cellular RA-binding proteins were detected in 2/2 lines tested. These findings suggest that, as a histological group, human neuroblastoma cells are extremely sensitive to RA-induced growth inhibition and morphological alterations generally associated with reduced expression of the malignant phenotype of this type of cancer.",
author = "Neil Sidell and Adrienne Altman and Haussler, {Mark R} and Seeger, {Robert C.}",
year = "1983",
month = "10",
day = "1",
doi = "10.1016/0014-4827(83)90184-2",
language = "English (US)",
volume = "148",
pages = "21--30",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Effects of retinoic acid (RA) on the growth and phenotypic expression of several human neuroblastoma cell lines

AU - Sidell, Neil

AU - Altman, Adrienne

AU - Haussler, Mark R

AU - Seeger, Robert C.

PY - 1983/10/1

Y1 - 1983/10/1

N2 - It has been shown that retinoic acid (RA) can promote morphologic differentiation and inhibit the growth of a human neuroblastoma cell line, LA-N-1. The present study tests the histological generality of these phenomena by determining the effects of RA on seven other human neuroblastoma cell lines. Results show that RA strongly inhibited anchorage-dependent growth and induced morphologic alterations in six of seven of the cell lines. These alterations included morphologic differentiation as evidenced by formation of neurite extensions in four of the lines, cellular enlargement and vacuolization in one culture, and formation of large, flattened epithelial or fibroblastic-like cells in another culture. Although one cell line was relatively insensitive to the effects of RA in monolayer culture, all seven were strongly inhibited by RA in soft agar assays. Cellular RA-binding proteins were detected in 2/2 lines tested. These findings suggest that, as a histological group, human neuroblastoma cells are extremely sensitive to RA-induced growth inhibition and morphological alterations generally associated with reduced expression of the malignant phenotype of this type of cancer.

AB - It has been shown that retinoic acid (RA) can promote morphologic differentiation and inhibit the growth of a human neuroblastoma cell line, LA-N-1. The present study tests the histological generality of these phenomena by determining the effects of RA on seven other human neuroblastoma cell lines. Results show that RA strongly inhibited anchorage-dependent growth and induced morphologic alterations in six of seven of the cell lines. These alterations included morphologic differentiation as evidenced by formation of neurite extensions in four of the lines, cellular enlargement and vacuolization in one culture, and formation of large, flattened epithelial or fibroblastic-like cells in another culture. Although one cell line was relatively insensitive to the effects of RA in monolayer culture, all seven were strongly inhibited by RA in soft agar assays. Cellular RA-binding proteins were detected in 2/2 lines tested. These findings suggest that, as a histological group, human neuroblastoma cells are extremely sensitive to RA-induced growth inhibition and morphological alterations generally associated with reduced expression of the malignant phenotype of this type of cancer.

UR - http://www.scopus.com/inward/record.url?scp=0021056873&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021056873&partnerID=8YFLogxK

U2 - 10.1016/0014-4827(83)90184-2

DO - 10.1016/0014-4827(83)90184-2

M3 - Article

C2 - 6313408

AN - SCOPUS:0021056873

VL - 148

SP - 21

EP - 30

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 1

ER -