Effects of subminimum inhibitory concentrations of antibiotics on the pasteurella multocida proteome: A systems approach

Bindu Nanduri, Mark L. Lawrence, Divya Swetha Peddinti, Shane C. Burgess

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

To identify key regulators of subminimum inhibitory concentration (sub-MIC) antibiotic response in the Pasteurella multocida proteome, we applied systems approaches. Using 2D-LC-ESI-MS2, we achieved 53% proteome coverage. To study the differential protein expression in response to sub-MIC antibiotics in the context of protein interaction networks, we inferred P. multocida Pm70 protein interaction network from orthologous proteins. We then overlaid the differential protein expression data onto the P. multocida protein interaction network to study the bacterial response. We identified proteins that could enhance antimicrobial activity. Overall compensatory response to antibiotics was characterized by altered expression of proteins involved in purine metabolism, stress response, and cell envelope permeability.

Original languageEnglish (US)
Article number254836
JournalComparative and Functional Genomics
Volume2008
DOIs
StatePublished - Jun 4 2008
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Genetics

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