Effects of subminimum inhibitory concentrations of antibiotics on the Pasteurella multocida proteome

Bindu Nanduri, Mark L. Lawrence, Carolyn E. Boyle, Mahalingam Ramkumar, Shane C. Burgess

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Subminimum inhibitory concentrations (sub-MICs) of antibiotics can be therapeutically effective, but the underlying molecular mechanisms are not well-characterized. We analyzed the Pasteurella multocida proteome response to sub-MICs of amoxicillin, chlortetracycline, and enrofloxacin using isotope-coded affinity tags (ICAT). There were parallel effects on inhibition of growth kinetics and suppression of protein expression by clusters of orthologous groups (COG) categories. Potential compensatory mechanisms enabling antibiotic adaptation were identified, including increased RecA expression caused by enrofloxacin.

Original languageEnglish (US)
Pages (from-to)572-580
Number of pages9
JournalJournal of Proteome Research
Volume5
Issue number3
DOIs
StatePublished - Mar 1 2006
Externally publishedYes

Keywords

  • Amoxicillin
  • Antibiotic
  • Bovine respiratory disease
  • Chlortetracycline
  • Clusters of orthologous groups
  • Enrofloxacin
  • ICAT
  • Isotope-coded affinity tag
  • RecA
  • Subminimum inhibitory concentration

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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