Effects of the steroid hormone 20-hydroxyecdysone and prior sensory input on the survival and growth of moth central olfactory neurons in vitro

Lynne A Oland, J. H. Hayashi

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Neurons in the developing (antennal) olfactory lobe of the moth Manduca sexta undergo a period of extensive process outgrowth and branching that coincides temporally with both a rising titer of the steroid hormone 20- hydroxyecdysone and the ingrowth of sensory axons from receptors in the antenna. To evaluate the contribution of these two influences to the morphological development of antennal-lobe neurons, we placed the neurons in cell culture. Antennal-lobe neurons were dissociated from normal and chronically unafferented lobes at different stages of development and were exposed to different doses of hormone. Six neuronal cell types with distinctive and stable morphologies appeared in cultures from all stages of pupal development. Morphological changes in these neuronal types were examined quantitatively by comparison of the total length and number of branches. We found that 20-hydroxyecdysone had little direct effect on the morphological development of antennal-lobe neurons, but brief exposure to sensory axons in vivo prior to dissociation significantly enhanced subsequent outgrowth in culture.

Original languageEnglish (US)
Pages (from-to)1170-1186
Number of pages17
JournalJournal of Neurobiology
Volume24
Issue number9
StatePublished - 1993

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Ecdysterone
Moths
Steroids
Hormones
Neurons
Growth
Axons
Manduca
Sensory Receptor Cells
Cell Culture Techniques
In Vitro Techniques

Keywords

  • 20-hydroxyecdysone
  • antennal-lobe neurons
  • sensory axons
  • steroid hormones

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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