TY - JOUR
T1 - Effects of transforming growth factor-beta (TGF-β1) on satellite cell activation and survival during oxidative stress
AU - Rathbone, Christopher R.
AU - Yamanouchi, Keitaro
AU - Chen, Xiaoyu K.
AU - Nevoret-Bell, Cedrine J.
AU - Rhoads, Robert P.
AU - Allen, Ronald E.
N1 - Funding Information:
Acknowledgments This work was supported by the National Institutes of Health (NIH) Grant AR053780 (CR); a post-doctoral fellowship from the Armed Forces Institute of Regenerative Medicine, administered through Wake Forest Institute of Regenerative Medicine, Winston-Salem, NC (XC); the Arizona Agriculture Experiment Station; and grants from the U.S. Department of Agriculture National Research Initiative Competitive Grant 2005-35206-15255 and Muscular Dystrophy Association Grant MDA3685 (RA). The opinions or assertions contained herein are the private views of the author and are not to be construed as official or as reflecting the views of the Department of the Army or the Department of Defense.
PY - 2011/9
Y1 - 2011/9
N2 - The regulation of adult skeletal muscle repair and regeneration is largely due to the contribution of resident adult myogenic precursor cells called satellite cells. The events preceding their participation in muscle repair include activation (exit from quiescence), proliferation, and differentiation. This study examined the effects of transforming growth factor-beta (TGF-β1) on satellite cell activation, determined whether TGF-β1 could maintain quiescence in the presence of hepatocyte growth factor (HGF), and whether the regulation of satellite cell activation with TGF-β1 improves the ability of satellite cells to withstand oxidative stress. The addition of TGF-β1 during early satellite cell activation (0-48 h) or during the proliferative phase (48-96 h) maintained and induced satellite cell quiescence, respectively, as determined by myogenic differentiation (MyoD) protein expression. TGF-β1 also attenuated satellite cell activation when used with HGF. Finally, the role of quiescence in protecting cells against oxidative stress was examined. TGF-β1 treatment and the low pH satellite cell preparation procedure, a technique that forestalls spontaneous activation in vitro, both enhanced survival of cultured satellite cells following hydrogen peroxide treatment. These findings indicate that TGF-β1 is capable of maintaining and inducing satellite cell quiescence and suggest methods to maintain satellite cell quiescence may improve their transplantation efficiency.
AB - The regulation of adult skeletal muscle repair and regeneration is largely due to the contribution of resident adult myogenic precursor cells called satellite cells. The events preceding their participation in muscle repair include activation (exit from quiescence), proliferation, and differentiation. This study examined the effects of transforming growth factor-beta (TGF-β1) on satellite cell activation, determined whether TGF-β1 could maintain quiescence in the presence of hepatocyte growth factor (HGF), and whether the regulation of satellite cell activation with TGF-β1 improves the ability of satellite cells to withstand oxidative stress. The addition of TGF-β1 during early satellite cell activation (0-48 h) or during the proliferative phase (48-96 h) maintained and induced satellite cell quiescence, respectively, as determined by myogenic differentiation (MyoD) protein expression. TGF-β1 also attenuated satellite cell activation when used with HGF. Finally, the role of quiescence in protecting cells against oxidative stress was examined. TGF-β1 treatment and the low pH satellite cell preparation procedure, a technique that forestalls spontaneous activation in vitro, both enhanced survival of cultured satellite cells following hydrogen peroxide treatment. These findings indicate that TGF-β1 is capable of maintaining and inducing satellite cell quiescence and suggest methods to maintain satellite cell quiescence may improve their transplantation efficiency.
KW - Oxidative stress
KW - Quiescence
KW - Satellite cell
KW - Skeletal muscle
KW - Transforming growth factor
UR - http://www.scopus.com/inward/record.url?scp=80054852603&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80054852603&partnerID=8YFLogxK
U2 - 10.1007/s10974-011-9255-8
DO - 10.1007/s10974-011-9255-8
M3 - Article
C2 - 21823037
AN - SCOPUS:80054852603
VL - 32
SP - 99
EP - 109
JO - Journal of Muscle Research and Cell Motility
JF - Journal of Muscle Research and Cell Motility
SN - 0142-4319
IS - 2
ER -