Effects of wheel running on glucose transporter (GLUT4) concentration in skeletal muscle of young adult and old rats

Eric A. Gulve, Kenneth J. Rodnick, Erik J Henriksen, John O. Holloszy

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

We examined the effects of voluntary exercise on glucose transporter concentration in skeletal muscle from young adult and old female Long-Evans rats. Rats had free access to voluntary running wheels beginning at 4 months of age or remained sedentary. Exercising rats ran - 7.5, 6.2, 5.6 and 5.3 km/day during their 6th, 8th, 9th and 10th month of age, respectively. During the 23rd, 24th and 25th month of age running distance averaged 3.0, 2.8 and 2.4 km/day, respectively. At 10 and 25 months of age, glucose transporter protein concentration was assessed in epitrochlearis and flexor digitorum brevis muscles with a polyclonal antibody directed against the GLUT4 transporter isoform.. GLUT4 protein concentration was not altered by the aging process (i.e., comparing 10- and 25-month-old rats) in either muscle type. Wheel running increased GLUT4 protein concentration by 45% in epitrochlearis muscles of 10-month-old rats relative to age-matched sedentary controls. The training-induced adaptation in GLUT4 protein was no longer present at age 25 months, probably because the running distance had declined by 50%. In the flexor digitorum brevis, exercise did not alter GLUT4 concentration at either 10 or 25 months, presumably due to insufficient recruitment of this muscle during wheel running as assessed by measurement of citrate synthase and hexokinase enzyme activities. Wheel running induced cardiac and soleus muscle hypertropy in 10- and 25-month-old rats. In summary, voluntary wheel running can induce an increase in skeletal muscle GLUT4 protein concentration in adult rats. Older rats that run less exhibit cardiac and soleus muscle hypertrophy, but do not maintain an elevated GLUT4 protein concentration in the epitrochlearic muscle. Aging does not alter GLUT4 protein concentration in the epitrochlearis or FDB muscles.

Original languageEnglish (US)
Pages (from-to)187-200
Number of pages14
JournalMechanisms of Ageing and Development
Volume67
Issue number1-2
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Glucose Transporter Type 4
Facilitative Glucose Transport Proteins
Running
Muscle
Rats
Young Adult
Wheels
Skeletal Muscle
Muscles
Myocardium
Exercise
Citrate (si)-Synthase
Long Evans Rats
Hexokinase
Muscle Proteins
Aging of materials
Hypertrophy
Protein Isoforms
Enzyme activity
Antibodies

ASJC Scopus subject areas

  • Aging
  • Biochemistry
  • Developmental Biology
  • Developmental Neuroscience

Cite this

Effects of wheel running on glucose transporter (GLUT4) concentration in skeletal muscle of young adult and old rats. / Gulve, Eric A.; Rodnick, Kenneth J.; Henriksen, Erik J; Holloszy, John O.

In: Mechanisms of Ageing and Development, Vol. 67, No. 1-2, 1993, p. 187-200.

Research output: Contribution to journalArticle

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abstract = "We examined the effects of voluntary exercise on glucose transporter concentration in skeletal muscle from young adult and old female Long-Evans rats. Rats had free access to voluntary running wheels beginning at 4 months of age or remained sedentary. Exercising rats ran - 7.5, 6.2, 5.6 and 5.3 km/day during their 6th, 8th, 9th and 10th month of age, respectively. During the 23rd, 24th and 25th month of age running distance averaged 3.0, 2.8 and 2.4 km/day, respectively. At 10 and 25 months of age, glucose transporter protein concentration was assessed in epitrochlearis and flexor digitorum brevis muscles with a polyclonal antibody directed against the GLUT4 transporter isoform.. GLUT4 protein concentration was not altered by the aging process (i.e., comparing 10- and 25-month-old rats) in either muscle type. Wheel running increased GLUT4 protein concentration by 45{\%} in epitrochlearis muscles of 10-month-old rats relative to age-matched sedentary controls. The training-induced adaptation in GLUT4 protein was no longer present at age 25 months, probably because the running distance had declined by 50{\%}. In the flexor digitorum brevis, exercise did not alter GLUT4 concentration at either 10 or 25 months, presumably due to insufficient recruitment of this muscle during wheel running as assessed by measurement of citrate synthase and hexokinase enzyme activities. Wheel running induced cardiac and soleus muscle hypertropy in 10- and 25-month-old rats. In summary, voluntary wheel running can induce an increase in skeletal muscle GLUT4 protein concentration in adult rats. Older rats that run less exhibit cardiac and soleus muscle hypertrophy, but do not maintain an elevated GLUT4 protein concentration in the epitrochlearic muscle. Aging does not alter GLUT4 protein concentration in the epitrochlearis or FDB muscles.",
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