Efficacy of a Dehydrated Steinernematid Nematode Against Black Cutworm (Lepidoptera: Noctuidae) and Diamondback Moth (Lepidoptera: Plutellidae)

Matthew E. Baur, Harry K. Kaya, Bruce E Tabashnik

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We compared the ability of in vitro-produced, commercially formulated with in vivo-produced, nonformulated Steinernema carpocapsae (Weiser) Poinar. All strain to infect and kill larvae of black cutworm, Agrotis ipsilon (Hufnagel), and diamondback moth, Plutella xylostella (L.). In vitro-produced nematodes formulated in wettable dispersible granules, which were stored dry, were rehydrated in water for 0-72 h before application. Against black cutworms, the efficacy of nematodes was (from most to least effective): in vivo > in vitro rehydrated for 72 h > in vitro rehydrated for 48 h > in vitro rehydrated for 24 h > dehydrated (0 h). Nematodes rehydrated for 72 h in water or moist soil were equally effective against black cutworm larvae, and both were significantly more effective than nematodes without rehydration. These results indicated that nematodes in the wettable dispersible granule formulation required time to rehydrate in the soil before infecting black cutworm larvae. Nematode treatments described above were applied to radish plants held at 100 or 75% RH and tested against diamondback moth larvae. At 100% RH, nematode efficacy was (from most to least effective): in vitro rehydrated for 72 h > in vivo > in vitro rehydrated 48 h > in vitro rehydrated 24 h > dehydrated (0 h). The efficacy of all treatments was lower at 75% than at 100% RH, and the ranking of in vivo and in vitro nematodes rehydrated for 72 h was reversed. The nematodes in the wettable dispersible granule formulation were effective for foliar treatments when humidity was high and nematodes were rehydrated for at least 48 h before application. The data show that nematode infectivity was reduced unless nematodes were rehydrated.

Original languageEnglish (US)
Pages (from-to)1200-1206
Number of pages7
JournalJournal of Economic Entomology
Volume90
Issue number5
StatePublished - Oct 1997

Fingerprint

Agrotis ipsilon
Plutellidae
Plutella xylostella
Noctuidae
moth
nematode
Lepidoptera
Nematoda
larva
granules
larvae
Steinernema carpocapsae
rehydration
infectivity
radishes
ranking
insect larvae
soil

Keywords

  • Agrotis ipsilon
  • Entomopathogenic
  • Formulation
  • Plutella xylostella
  • Steinernema carpocapsae
  • Steinernematidae

ASJC Scopus subject areas

  • Insect Science

Cite this

Efficacy of a Dehydrated Steinernematid Nematode Against Black Cutworm (Lepidoptera : Noctuidae) and Diamondback Moth (Lepidoptera: Plutellidae). / Baur, Matthew E.; Kaya, Harry K.; Tabashnik, Bruce E.

In: Journal of Economic Entomology, Vol. 90, No. 5, 10.1997, p. 1200-1206.

Research output: Contribution to journalArticle

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abstract = "We compared the ability of in vitro-produced, commercially formulated with in vivo-produced, nonformulated Steinernema carpocapsae (Weiser) Poinar. All strain to infect and kill larvae of black cutworm, Agrotis ipsilon (Hufnagel), and diamondback moth, Plutella xylostella (L.). In vitro-produced nematodes formulated in wettable dispersible granules, which were stored dry, were rehydrated in water for 0-72 h before application. Against black cutworms, the efficacy of nematodes was (from most to least effective): in vivo > in vitro rehydrated for 72 h > in vitro rehydrated for 48 h > in vitro rehydrated for 24 h > dehydrated (0 h). Nematodes rehydrated for 72 h in water or moist soil were equally effective against black cutworm larvae, and both were significantly more effective than nematodes without rehydration. These results indicated that nematodes in the wettable dispersible granule formulation required time to rehydrate in the soil before infecting black cutworm larvae. Nematode treatments described above were applied to radish plants held at 100 or 75{\%} RH and tested against diamondback moth larvae. At 100{\%} RH, nematode efficacy was (from most to least effective): in vitro rehydrated for 72 h > in vivo > in vitro rehydrated 48 h > in vitro rehydrated 24 h > dehydrated (0 h). The efficacy of all treatments was lower at 75{\%} than at 100{\%} RH, and the ranking of in vivo and in vitro nematodes rehydrated for 72 h was reversed. The nematodes in the wettable dispersible granule formulation were effective for foliar treatments when humidity was high and nematodes were rehydrated for at least 48 h before application. The data show that nematode infectivity was reduced unless nematodes were rehydrated.",
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