Efficacy of genetically modified Bt toxins against insects with different genetic mechanisms of resistance

Bruce E Tabashnik, Fangneng Huang, Mukti N. Ghimire, B. Rogers Leonard, Blair D. Siegfried, Murugesan Rangasamy, Yajun Yang, Yidong Wu, Linda J. Gahan, David G. Heckel, Alejandra Bravo, Mario Soberón

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Abstract

Transgenic crops that produce Bacillus thuringiensis (Bt) toxins are grown widely for pest control, but insect adaptation can reduce their efficacy. The genetically modified Bt toxins Cry1AbMod and Cry1AcMod were designed to counter insect resistance to native Bt toxins Cry1Ab and Cry1Ac. Previous results suggested that the modified toxins would be effective only if resistance was linked with mutations in genes encoding toxin-binding cadherin proteins. Here we report evidence from five major crop pests refuting this hypothesis. Relative to native toxins, the potency of modified toxins was >350-fold higher against resistant strains of Plutella xylostella and Ostrinia nubilalis in which resistance was not linked with cadherin mutations. Conversely, the modified toxins provided little or no advantage against some resistant strains of three other pests with altered cadherin. Independent of the presence of cadherin mutations, the relative potency of the modified toxins was generally higher against the most resistant strains.

Original languageEnglish (US)
Pages (from-to)1128-1131
Number of pages4
JournalNature Biotechnology
Volume29
Issue number12
DOIs
StatePublished - Dec 2011

Fingerprint

Bacillus thuringiensis
Bacilli
Cadherins
Insects
Crops
Pest control
Mutation
Gene encoding
Pest Control
Carrier Proteins
Genes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biotechnology
  • Molecular Medicine
  • Bioengineering
  • Biomedical Engineering

Cite this

Tabashnik, B. E., Huang, F., Ghimire, M. N., Leonard, B. R., Siegfried, B. D., Rangasamy, M., ... Soberón, M. (2011). Efficacy of genetically modified Bt toxins against insects with different genetic mechanisms of resistance. Nature Biotechnology, 29(12), 1128-1131. https://doi.org/10.1038/nbt.1988

Efficacy of genetically modified Bt toxins against insects with different genetic mechanisms of resistance. / Tabashnik, Bruce E; Huang, Fangneng; Ghimire, Mukti N.; Leonard, B. Rogers; Siegfried, Blair D.; Rangasamy, Murugesan; Yang, Yajun; Wu, Yidong; Gahan, Linda J.; Heckel, David G.; Bravo, Alejandra; Soberón, Mario.

In: Nature Biotechnology, Vol. 29, No. 12, 12.2011, p. 1128-1131.

Research output: Contribution to journalArticle

Tabashnik, BE, Huang, F, Ghimire, MN, Leonard, BR, Siegfried, BD, Rangasamy, M, Yang, Y, Wu, Y, Gahan, LJ, Heckel, DG, Bravo, A & Soberón, M 2011, 'Efficacy of genetically modified Bt toxins against insects with different genetic mechanisms of resistance', Nature Biotechnology, vol. 29, no. 12, pp. 1128-1131. https://doi.org/10.1038/nbt.1988
Tabashnik, Bruce E ; Huang, Fangneng ; Ghimire, Mukti N. ; Leonard, B. Rogers ; Siegfried, Blair D. ; Rangasamy, Murugesan ; Yang, Yajun ; Wu, Yidong ; Gahan, Linda J. ; Heckel, David G. ; Bravo, Alejandra ; Soberón, Mario. / Efficacy of genetically modified Bt toxins against insects with different genetic mechanisms of resistance. In: Nature Biotechnology. 2011 ; Vol. 29, No. 12. pp. 1128-1131.
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