Efficacy of roselle (Hibiscus sabdariffa) calyx formulations against Escherichia coli O157: H7 during flume-washing of organic leafy greens

D. Jaroni, R. Kakani, Sadhana Ravishankar, R. Jadeja

Research output: Contribution to journalArticle

Abstract

Foodborne outbreaks due to contaminated fresh produce, including organic leafy greens, have increased in the last twenty years. Several of these outbreaks have been associated with Escherichia coli O157:H7 infections. The limited availability of approved antimicrobial washes for organic leafy greens warrants the need to seek alternative approaches by the organic produce industry. The present study evaluated the use of roselle (Hibiscus sabdariffa) calyx formulations as flume-tank wash treatments for four types of organic leafy greens (iceberg and romaine lettuce, and mature and baby spinach). These formulations were prepared as either 10, 20, or 30% concentrations (v/v) of roselle aqueous extract or as 100% concentration (v/v) of roselle tea. Leafy greens were inoculated with a cocktail of 3 E. coli O157:H7 strains and washed with appropriate antimicrobial treatments. Treated leafy greens were tested for surviving E. coli O157:H7 populations on 0, 1, and 3 days. Results from the study indicated a 1.4-2.5 log cfu (colony forming units)/g reduction overall for the 20% concentration of roselle extract by day 3, on all leafy greens. Baby spinach treated with 30% roselle extract showed a 3.7 log cfu/g reduction in pathogen population by day 3. Treatment with roselle tea resulted in 2.0-3.4 log cfu/g reductions of E. coli O157:H7 for all leafy greens by the third day. This study demonstrates the potential of roselle calyx formulations for reduction of E. coli O157:H7 populations during flume-washing of organic leafy greens.

Original languageEnglish (US)
Pages (from-to)115-121
Number of pages7
JournalQuality Assurance and Safety of Crops and Foods
Volume9
Issue number1
DOIs
StatePublished - 2017

Fingerprint

Hibiscus
Hibiscus sabdariffa
Escherichia coli O157
green leafy vegetables
calyx
washing
Spinacia oleracea
Stem Cells
Tea
spinach
tea
Disease Outbreaks
extracts
anti-infective agents
Organic Food
romaine lettuce
Population
Ice Cover
Lactuca sativa var. capitata
Lettuce

Keywords

  • Antimicrobials
  • Foodborne pathogens
  • Fresh produce
  • Inhibition
  • Plant extracts
  • Wash-water

ASJC Scopus subject areas

  • Food Science
  • Agronomy and Crop Science

Cite this

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title = "Efficacy of roselle (Hibiscus sabdariffa) calyx formulations against Escherichia coli O157: H7 during flume-washing of organic leafy greens",
abstract = "Foodborne outbreaks due to contaminated fresh produce, including organic leafy greens, have increased in the last twenty years. Several of these outbreaks have been associated with Escherichia coli O157:H7 infections. The limited availability of approved antimicrobial washes for organic leafy greens warrants the need to seek alternative approaches by the organic produce industry. The present study evaluated the use of roselle (Hibiscus sabdariffa) calyx formulations as flume-tank wash treatments for four types of organic leafy greens (iceberg and romaine lettuce, and mature and baby spinach). These formulations were prepared as either 10, 20, or 30{\%} concentrations (v/v) of roselle aqueous extract or as 100{\%} concentration (v/v) of roselle tea. Leafy greens were inoculated with a cocktail of 3 E. coli O157:H7 strains and washed with appropriate antimicrobial treatments. Treated leafy greens were tested for surviving E. coli O157:H7 populations on 0, 1, and 3 days. Results from the study indicated a 1.4-2.5 log cfu (colony forming units)/g reduction overall for the 20{\%} concentration of roselle extract by day 3, on all leafy greens. Baby spinach treated with 30{\%} roselle extract showed a 3.7 log cfu/g reduction in pathogen population by day 3. Treatment with roselle tea resulted in 2.0-3.4 log cfu/g reductions of E. coli O157:H7 for all leafy greens by the third day. This study demonstrates the potential of roselle calyx formulations for reduction of E. coli O157:H7 populations during flume-washing of organic leafy greens.",
keywords = "Antimicrobials, Foodborne pathogens, Fresh produce, Inhibition, Plant extracts, Wash-water",
author = "D. Jaroni and R. Kakani and Sadhana Ravishankar and R. Jadeja",
year = "2017",
doi = "10.3920/QAS2015.0679",
language = "English (US)",
volume = "9",
pages = "115--121",
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T1 - Efficacy of roselle (Hibiscus sabdariffa) calyx formulations against Escherichia coli O157

T2 - H7 during flume-washing of organic leafy greens

AU - Jaroni, D.

AU - Kakani, R.

AU - Ravishankar, Sadhana

AU - Jadeja, R.

PY - 2017

Y1 - 2017

N2 - Foodborne outbreaks due to contaminated fresh produce, including organic leafy greens, have increased in the last twenty years. Several of these outbreaks have been associated with Escherichia coli O157:H7 infections. The limited availability of approved antimicrobial washes for organic leafy greens warrants the need to seek alternative approaches by the organic produce industry. The present study evaluated the use of roselle (Hibiscus sabdariffa) calyx formulations as flume-tank wash treatments for four types of organic leafy greens (iceberg and romaine lettuce, and mature and baby spinach). These formulations were prepared as either 10, 20, or 30% concentrations (v/v) of roselle aqueous extract or as 100% concentration (v/v) of roselle tea. Leafy greens were inoculated with a cocktail of 3 E. coli O157:H7 strains and washed with appropriate antimicrobial treatments. Treated leafy greens were tested for surviving E. coli O157:H7 populations on 0, 1, and 3 days. Results from the study indicated a 1.4-2.5 log cfu (colony forming units)/g reduction overall for the 20% concentration of roselle extract by day 3, on all leafy greens. Baby spinach treated with 30% roselle extract showed a 3.7 log cfu/g reduction in pathogen population by day 3. Treatment with roselle tea resulted in 2.0-3.4 log cfu/g reductions of E. coli O157:H7 for all leafy greens by the third day. This study demonstrates the potential of roselle calyx formulations for reduction of E. coli O157:H7 populations during flume-washing of organic leafy greens.

AB - Foodborne outbreaks due to contaminated fresh produce, including organic leafy greens, have increased in the last twenty years. Several of these outbreaks have been associated with Escherichia coli O157:H7 infections. The limited availability of approved antimicrobial washes for organic leafy greens warrants the need to seek alternative approaches by the organic produce industry. The present study evaluated the use of roselle (Hibiscus sabdariffa) calyx formulations as flume-tank wash treatments for four types of organic leafy greens (iceberg and romaine lettuce, and mature and baby spinach). These formulations were prepared as either 10, 20, or 30% concentrations (v/v) of roselle aqueous extract or as 100% concentration (v/v) of roselle tea. Leafy greens were inoculated with a cocktail of 3 E. coli O157:H7 strains and washed with appropriate antimicrobial treatments. Treated leafy greens were tested for surviving E. coli O157:H7 populations on 0, 1, and 3 days. Results from the study indicated a 1.4-2.5 log cfu (colony forming units)/g reduction overall for the 20% concentration of roselle extract by day 3, on all leafy greens. Baby spinach treated with 30% roselle extract showed a 3.7 log cfu/g reduction in pathogen population by day 3. Treatment with roselle tea resulted in 2.0-3.4 log cfu/g reductions of E. coli O157:H7 for all leafy greens by the third day. This study demonstrates the potential of roselle calyx formulations for reduction of E. coli O157:H7 populations during flume-washing of organic leafy greens.

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