Efficient purification of the biosurfactant viscosin from Pseudomonas libanensis strain M9-3 and its physicochemical and biological properties

Harvinder S. Saini, Blanca E. Barragán-Huerta, Ariel Lebrón-Paler, Jeanne E Pemberton, Refugio R. Vázquez, Anna M. Burns, Marilyn T. Marron, Chistopher J. Seliga, Leslie Gunatilaka, Raina Margaret Maier

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Viscosin (1), an effective surface-active cyclic lipopeptide, was efficiently recovered from Pseudomonas libanensis M9-3 with a simple purification protocol. A major pigment also obtained during this process was identified as phenazine-1-carboxylic acid. The critical micelle concentration (cmc) of viscosin was determined to be 54 mg L-1, and the minimum surface tension between air and water at the cmc was 28 mN m-1. Viscosin forms stable emulsions even at low concentrations (7.5 mg L -1), and the conditional stability constant for a cadmium - viscosin complex was determined to be 5.87. The physicochemical properties measured for viscosin are similar to other well-studied biosurfactants such as rhamnolipid and surfactin. Viscosin inhibited migration of the metastatic prostate cancer cell line, PC-3M, without visible toxicity. These properties suggest the potential of viscosin in environmental and biomedical applications.

Original languageEnglish (US)
Pages (from-to)1011-1015
Number of pages5
JournalJournal of Natural Products
Volume71
Issue number6
DOIs
StatePublished - Jun 2008

Fingerprint

biosurfactants
micelles
Pseudomonas
Purification
surfactin
phenazines
lipopeptides
surface tension
prostatic neoplasms
carboxylic acids
emulsions
cadmium
physicochemical properties
pigments
cell lines
toxicity
Critical micelle concentration
air
Micelles
Lipopeptides

ASJC Scopus subject areas

  • Plant Science
  • Chemistry (miscellaneous)
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this

Efficient purification of the biosurfactant viscosin from Pseudomonas libanensis strain M9-3 and its physicochemical and biological properties. / Saini, Harvinder S.; Barragán-Huerta, Blanca E.; Lebrón-Paler, Ariel; Pemberton, Jeanne E; Vázquez, Refugio R.; Burns, Anna M.; Marron, Marilyn T.; Seliga, Chistopher J.; Gunatilaka, Leslie; Maier, Raina Margaret.

In: Journal of Natural Products, Vol. 71, No. 6, 06.2008, p. 1011-1015.

Research output: Contribution to journalArticle

Saini, Harvinder S. ; Barragán-Huerta, Blanca E. ; Lebrón-Paler, Ariel ; Pemberton, Jeanne E ; Vázquez, Refugio R. ; Burns, Anna M. ; Marron, Marilyn T. ; Seliga, Chistopher J. ; Gunatilaka, Leslie ; Maier, Raina Margaret. / Efficient purification of the biosurfactant viscosin from Pseudomonas libanensis strain M9-3 and its physicochemical and biological properties. In: Journal of Natural Products. 2008 ; Vol. 71, No. 6. pp. 1011-1015.
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