Efficient purification of the biosurfactant viscosin from Pseudomonas libanensis strain M9-3 and its physicochemical and biological properties

Harvinder S. Saini, Blanca E. Barragán-Huerta, Ariel Lebrón-Paler, Jeanne E. Pemberton, Refugio R. Vázquez, Anna M. Burns, Marilyn T. Marron, Chistopher J. Seliga, A. A.Leslie Gunatilaka, Raina M. Maier

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Viscosin (1), an effective surface-active cyclic lipopeptide, was efficiently recovered from Pseudomonas libanensis M9-3 with a simple purification protocol. A major pigment also obtained during this process was identified as phenazine-1-carboxylic acid. The critical micelle concentration (cmc) of viscosin was determined to be 54 mg L-1, and the minimum surface tension between air and water at the cmc was 28 mN m-1. Viscosin forms stable emulsions even at low concentrations (7.5 mg L -1), and the conditional stability constant for a cadmium - viscosin complex was determined to be 5.87. The physicochemical properties measured for viscosin are similar to other well-studied biosurfactants such as rhamnolipid and surfactin. Viscosin inhibited migration of the metastatic prostate cancer cell line, PC-3M, without visible toxicity. These properties suggest the potential of viscosin in environmental and biomedical applications.

Original languageEnglish (US)
Pages (from-to)1011-1015
Number of pages5
JournalJournal Of Natural Products
Volume71
Issue number6
DOIs
StatePublished - Jun 2008

ASJC Scopus subject areas

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

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