Efficient purification of the biosurfactant viscosin from Pseudomonas libanensis strain M9-3 and its physicochemical and biological properties

Harvinder S. Saini, Blanca E. Barragán-Huerta, Ariel Lebrón-Paler, Jeanne E Pemberton, Refugio R. Vázquez, Anna M. Burns, Marilyn T. Marron, Chistopher J. Seliga, Leslie Gunatilaka, Raina Margaret Maier

Research output: Contribution to journalArticle

33 Scopus citations


Viscosin (1), an effective surface-active cyclic lipopeptide, was efficiently recovered from Pseudomonas libanensis M9-3 with a simple purification protocol. A major pigment also obtained during this process was identified as phenazine-1-carboxylic acid. The critical micelle concentration (cmc) of viscosin was determined to be 54 mg L-1, and the minimum surface tension between air and water at the cmc was 28 mN m-1. Viscosin forms stable emulsions even at low concentrations (7.5 mg L -1), and the conditional stability constant for a cadmium - viscosin complex was determined to be 5.87. The physicochemical properties measured for viscosin are similar to other well-studied biosurfactants such as rhamnolipid and surfactin. Viscosin inhibited migration of the metastatic prostate cancer cell line, PC-3M, without visible toxicity. These properties suggest the potential of viscosin in environmental and biomedical applications.

Original languageEnglish (US)
Pages (from-to)1011-1015
Number of pages5
JournalJournal of Natural Products
Issue number6
Publication statusPublished - Jun 2008


ASJC Scopus subject areas

  • Plant Science
  • Chemistry (miscellaneous)
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this