EGF induces the expression of matrilysin in the human prostate adenocarcinoma cell line, LNCAP

Padma Sundareshan, Raymond B Nagle, G. Tim Bowden

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

BACKGROUND. Matrix metalloproteinases (MMPs) are regulated both positively and negatively at the transcriptional level by a variety of growth factors, oncogenes, and tumor promoters. Induction of the MMP, matrilysin, by epidermal growth factor (EGF) was investigated in a human prostate cancer cell line. METHODS. Secreted protein and messenger RNA were detected using Western and Northern methods, respectively. EGF receptor antibodies were used for neutralization of the EGF receptor to determine the role of the EGF growth factor family (EGF, transforming growth factor α (TGFα), or amphiregulin) in the basal induction of matrilysin. RESULTS. EGF increased mRNA and secreted protein levels for the MMP matrilysin in LNCaP cells, in a concentration- and time-dependent manner. Transforming growth factor β1 (TGFβ1) had no inhibitory effect on the levels of mRNA or secreted protein induced by EGF in LNCaP cells. Decay of matrilysin mRNA after the addition of actinomycin D indicated that the half-life of matrilysin mRNA was not altered by EGF. Blocking with a neutralizing antibody to the EGF receptor did not alter the basal level of secreted matrilysin. CONCLUSIONS. Exogenously added EGF increased matrilysin mRNA, perhaps at a transcriptional level. Growth factors, other than the members of the EGF family which act through the EGF receptor, may be involved in the regulation of the basal level of secreted matrilysin in LNCaP cells. Our data with LNCaP cells suggest that paracrine regulation of matrilysin expression in human prostate carcinoma cells could be via the EGF receptor.

Original languageEnglish (US)
Pages (from-to)159-166
Number of pages8
JournalProstate
Volume40
Issue number3
DOIs
StatePublished - Aug 1 1999

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Matrix Metalloproteinase 7
Epidermal Growth Factor
Prostate
Adenocarcinoma
Cell Line
Epidermal Growth Factor Receptor
Messenger RNA
Matrix Metalloproteinases
Intercellular Signaling Peptides and Proteins
Transforming Growth Factors
Proteins
RNA Stability
Dactinomycin
Neutralizing Antibodies
Oncogenes
Carcinogens
Half-Life
Prostatic Neoplasms
Carcinoma

Keywords

  • EGF
  • LNCaP
  • Matrilysin
  • mRNA stability
  • Prostate cancer

ASJC Scopus subject areas

  • Urology

Cite this

EGF induces the expression of matrilysin in the human prostate adenocarcinoma cell line, LNCAP. / Sundareshan, Padma; Nagle, Raymond B; Bowden, G. Tim.

In: Prostate, Vol. 40, No. 3, 01.08.1999, p. 159-166.

Research output: Contribution to journalArticle

Sundareshan, Padma ; Nagle, Raymond B ; Bowden, G. Tim. / EGF induces the expression of matrilysin in the human prostate adenocarcinoma cell line, LNCAP. In: Prostate. 1999 ; Vol. 40, No. 3. pp. 159-166.
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abstract = "BACKGROUND. Matrix metalloproteinases (MMPs) are regulated both positively and negatively at the transcriptional level by a variety of growth factors, oncogenes, and tumor promoters. Induction of the MMP, matrilysin, by epidermal growth factor (EGF) was investigated in a human prostate cancer cell line. METHODS. Secreted protein and messenger RNA were detected using Western and Northern methods, respectively. EGF receptor antibodies were used for neutralization of the EGF receptor to determine the role of the EGF growth factor family (EGF, transforming growth factor α (TGFα), or amphiregulin) in the basal induction of matrilysin. RESULTS. EGF increased mRNA and secreted protein levels for the MMP matrilysin in LNCaP cells, in a concentration- and time-dependent manner. Transforming growth factor β1 (TGFβ1) had no inhibitory effect on the levels of mRNA or secreted protein induced by EGF in LNCaP cells. Decay of matrilysin mRNA after the addition of actinomycin D indicated that the half-life of matrilysin mRNA was not altered by EGF. Blocking with a neutralizing antibody to the EGF receptor did not alter the basal level of secreted matrilysin. CONCLUSIONS. Exogenously added EGF increased matrilysin mRNA, perhaps at a transcriptional level. Growth factors, other than the members of the EGF family which act through the EGF receptor, may be involved in the regulation of the basal level of secreted matrilysin in LNCaP cells. Our data with LNCaP cells suggest that paracrine regulation of matrilysin expression in human prostate carcinoma cells could be via the EGF receptor.",
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N2 - BACKGROUND. Matrix metalloproteinases (MMPs) are regulated both positively and negatively at the transcriptional level by a variety of growth factors, oncogenes, and tumor promoters. Induction of the MMP, matrilysin, by epidermal growth factor (EGF) was investigated in a human prostate cancer cell line. METHODS. Secreted protein and messenger RNA were detected using Western and Northern methods, respectively. EGF receptor antibodies were used for neutralization of the EGF receptor to determine the role of the EGF growth factor family (EGF, transforming growth factor α (TGFα), or amphiregulin) in the basal induction of matrilysin. RESULTS. EGF increased mRNA and secreted protein levels for the MMP matrilysin in LNCaP cells, in a concentration- and time-dependent manner. Transforming growth factor β1 (TGFβ1) had no inhibitory effect on the levels of mRNA or secreted protein induced by EGF in LNCaP cells. Decay of matrilysin mRNA after the addition of actinomycin D indicated that the half-life of matrilysin mRNA was not altered by EGF. Blocking with a neutralizing antibody to the EGF receptor did not alter the basal level of secreted matrilysin. CONCLUSIONS. Exogenously added EGF increased matrilysin mRNA, perhaps at a transcriptional level. Growth factors, other than the members of the EGF family which act through the EGF receptor, may be involved in the regulation of the basal level of secreted matrilysin in LNCaP cells. Our data with LNCaP cells suggest that paracrine regulation of matrilysin expression in human prostate carcinoma cells could be via the EGF receptor.

AB - BACKGROUND. Matrix metalloproteinases (MMPs) are regulated both positively and negatively at the transcriptional level by a variety of growth factors, oncogenes, and tumor promoters. Induction of the MMP, matrilysin, by epidermal growth factor (EGF) was investigated in a human prostate cancer cell line. METHODS. Secreted protein and messenger RNA were detected using Western and Northern methods, respectively. EGF receptor antibodies were used for neutralization of the EGF receptor to determine the role of the EGF growth factor family (EGF, transforming growth factor α (TGFα), or amphiregulin) in the basal induction of matrilysin. RESULTS. EGF increased mRNA and secreted protein levels for the MMP matrilysin in LNCaP cells, in a concentration- and time-dependent manner. Transforming growth factor β1 (TGFβ1) had no inhibitory effect on the levels of mRNA or secreted protein induced by EGF in LNCaP cells. Decay of matrilysin mRNA after the addition of actinomycin D indicated that the half-life of matrilysin mRNA was not altered by EGF. Blocking with a neutralizing antibody to the EGF receptor did not alter the basal level of secreted matrilysin. CONCLUSIONS. Exogenously added EGF increased matrilysin mRNA, perhaps at a transcriptional level. Growth factors, other than the members of the EGF family which act through the EGF receptor, may be involved in the regulation of the basal level of secreted matrilysin in LNCaP cells. Our data with LNCaP cells suggest that paracrine regulation of matrilysin expression in human prostate carcinoma cells could be via the EGF receptor.

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