Elastic deformation and area per lipid of membranes: Atomistic view from solid-state deuterium NMR spectroscopy

Jacob J. Kinnun, K. J. Mallikarjunaiah, Horia I. Petrache, Michael F Brown

Research output: Contribution to journalReview article

23 Citations (Scopus)

Abstract

This article reviews the application of solid-state 2H nuclear magnetic resonance (NMR) spectroscopy for investigating the deformation of lipid bilayers at the atomistic level. For liquid-crystalline membranes, the average structure is manifested by the segmental order parameters (SCD) of the lipids. Solid-state 2H NMR yields observables directly related to the stress field of the lipid bilayer. The extent to which lipid bilayers are deformed by osmotic pressure is integral to how lipid-protein interactions affect membrane functions. Calculations of the average area per lipid and related structural properties are pertinent to bilayer remodeling and molecular dynamics (MD) simulations of membranes. To establish structural quantities, such as area per lipid and volumetric bilayer thickness, a mean-torque analysis of 2H NMR order parameters is applied. Osmotic stress is introduced by adding polymer solutions or by gravimetric dehydration, which are thermodynamically equivalent. Solid-state NMR studies of lipids under osmotic stress probe membrane interactions involving collective bilayer undulations, order-director fluctuations, and lipid molecular protrusions. Removal of water yields a reduction of the mean area per lipid, with a corresponding increase in volumetric bilayer thickness, by up to 20% in the liquid-crystalline state. Hydrophobic mismatch can shift protein states involving mechanosensation, transport, and molecular recognition by G-protein-coupled receptors. Measurements of the order parameters versus osmotic pressure yield the elastic area compressibility modulus and the corresponding bilayer thickness at an atomistic level. Solid-state 2H NMR thus reveals how membrane deformation can affect protein conformational changes within the stress field of the lipid bilayer. This article is part of a Special Issue entitled: NMR Spectroscopy for Atomistic Views of Biomembranes and Cell Surfaces. Guest Editors: Lynette Cegelski and David P. Weliky.

Original languageEnglish (US)
Pages (from-to)246-259
Number of pages14
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1848
Issue number1
DOIs
StatePublished - 2015

Fingerprint

Deuterium
Elastic deformation
Membrane Lipids
Nuclear magnetic resonance spectroscopy
Lipid Bilayers
Magnetic Resonance Spectroscopy
Osmotic Pressure
Lipid bilayers
Lipids
Membranes
Nuclear magnetic resonance
Proteins
Crystalline materials
Torque
Molecular recognition
Molecular Dynamics Simulation
G-Protein-Coupled Receptors
Dehydration
Liquids
Polymer solutions

Keywords

  • Area per lipid
  • Lipid-protein interaction
  • Molecular dynamics
  • Order parameter
  • Osmotic pressure
  • Solid-state NMR

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

Elastic deformation and area per lipid of membranes : Atomistic view from solid-state deuterium NMR spectroscopy. / Kinnun, Jacob J.; Mallikarjunaiah, K. J.; Petrache, Horia I.; Brown, Michael F.

In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1848, No. 1, 2015, p. 246-259.

Research output: Contribution to journalReview article

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abstract = "This article reviews the application of solid-state 2H nuclear magnetic resonance (NMR) spectroscopy for investigating the deformation of lipid bilayers at the atomistic level. For liquid-crystalline membranes, the average structure is manifested by the segmental order parameters (SCD) of the lipids. Solid-state 2H NMR yields observables directly related to the stress field of the lipid bilayer. The extent to which lipid bilayers are deformed by osmotic pressure is integral to how lipid-protein interactions affect membrane functions. Calculations of the average area per lipid and related structural properties are pertinent to bilayer remodeling and molecular dynamics (MD) simulations of membranes. To establish structural quantities, such as area per lipid and volumetric bilayer thickness, a mean-torque analysis of 2H NMR order parameters is applied. Osmotic stress is introduced by adding polymer solutions or by gravimetric dehydration, which are thermodynamically equivalent. Solid-state NMR studies of lipids under osmotic stress probe membrane interactions involving collective bilayer undulations, order-director fluctuations, and lipid molecular protrusions. Removal of water yields a reduction of the mean area per lipid, with a corresponding increase in volumetric bilayer thickness, by up to 20{\%} in the liquid-crystalline state. Hydrophobic mismatch can shift protein states involving mechanosensation, transport, and molecular recognition by G-protein-coupled receptors. Measurements of the order parameters versus osmotic pressure yield the elastic area compressibility modulus and the corresponding bilayer thickness at an atomistic level. Solid-state 2H NMR thus reveals how membrane deformation can affect protein conformational changes within the stress field of the lipid bilayer. This article is part of a Special Issue entitled: NMR Spectroscopy for Atomistic Views of Biomembranes and Cell Surfaces. Guest Editors: Lynette Cegelski and David P. Weliky.",
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