Electrical molecular focusing for laser induced fluorescence based single DNA detection

Tza Huei Wang, Pak Kin Wong, Chih Ming Ho

Research output: Chapter in Book/Report/Conference proceedingConference contribution

11 Citations (Scopus)

Abstract

This paper details an electrokinetic focusing technique that significantly enhances the detection efficiency for laser induced fluorescence (LIF) based molecular sensing by concentrating fluorescence labeled molecules in a tiny probe region. 3-D electrodes were fabricated to generate wider and denser electric fields toward the probe region in the microchannel. The generated electric field is able to focus flowing DNA molecules to widths as narrow as 3 μm in a 120 μm wide channel. A single molecule detection technique is developed and the enhancement of detection efficiency by using the focusing method is demonstrated.

Original languageEnglish (US)
Title of host publicationProceedings of the IEEE Micro Electro Mechanical Systems (MEMS)
Pages15-18
Number of pages4
StatePublished - 2002
Externally publishedYes
Event15th IEEE International Conference on Micro Electro Mechanical Systems MEMS 2002 - Las Vegas, NV, United States
Duration: Jan 20 2002Jan 24 2002

Other

Other15th IEEE International Conference on Micro Electro Mechanical Systems MEMS 2002
CountryUnited States
CityLas Vegas, NV
Period1/20/021/24/02

Fingerprint

DNA
Fluorescence
Molecules
Lasers
Electric fields
Microchannels
Electrodes

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Electrical and Electronic Engineering
  • Mechanical Engineering

Cite this

Wang, T. H., Wong, P. K., & Ho, C. M. (2002). Electrical molecular focusing for laser induced fluorescence based single DNA detection. In Proceedings of the IEEE Micro Electro Mechanical Systems (MEMS) (pp. 15-18)

Electrical molecular focusing for laser induced fluorescence based single DNA detection. / Wang, Tza Huei; Wong, Pak Kin; Ho, Chih Ming.

Proceedings of the IEEE Micro Electro Mechanical Systems (MEMS). 2002. p. 15-18.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Wang, TH, Wong, PK & Ho, CM 2002, Electrical molecular focusing for laser induced fluorescence based single DNA detection. in Proceedings of the IEEE Micro Electro Mechanical Systems (MEMS). pp. 15-18, 15th IEEE International Conference on Micro Electro Mechanical Systems MEMS 2002, Las Vegas, NV, United States, 1/20/02.
Wang TH, Wong PK, Ho CM. Electrical molecular focusing for laser induced fluorescence based single DNA detection. In Proceedings of the IEEE Micro Electro Mechanical Systems (MEMS). 2002. p. 15-18
Wang, Tza Huei ; Wong, Pak Kin ; Ho, Chih Ming. / Electrical molecular focusing for laser induced fluorescence based single DNA detection. Proceedings of the IEEE Micro Electro Mechanical Systems (MEMS). 2002. pp. 15-18
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